Incredible affinity of Kattosh with PPAR‐γ receptors attenuates STZ‐induced pancreas and kidney lesions evidenced in chemicobiological interactions

Abstract

Since ancient times, plants have been used as green bioresources to ensure a healthier life by recovering from different diseases. Kattosh (Lasia spinosa L. Thwaites) is a local plant with various traditional uses, especially for arthritis, constipation and coughs. This research investigated the effect of Kattosh stem extract (LSES) on streptozotocin‐induced damage to the pancreas, kidney, and liver using in vitro, in vivo and in silico methods. In vitro phytochemical, antioxidative and anti‐inflammatory effects of LSES were accomplished by established methods followed by antidiabetic actions in in vivo randomized controlled intervention in STZ‐induced animal models for four weeks. In an in silico study, LSES phytocompounds interacted with antidiabetic receptors of peroxisome proliferator‐activated receptor‐gamma (PPAR, PDB ID: 3G9E), AMP‐activated protein kinase (AMPK, PDB ID: 4CFH) and α‐amylase enzyme (PDB ID: 1PPI) to verify the in vivo results. In addition, LSES showed promising in vitro antioxidative and anti‐inflammatory effects. In contrast, it showed a decrease in weekly blood glucose level, normalized lipid profile, ameliorated liver and cardiac markers, managed serum AST and ALT levels, and increased glucose tolerance ability in the animal model study. Restoration of pancreatic and kidney damage was reflected by improving histopathological images. In ligand–receptor interaction, ethyl α‐d‐glucopyranoside of Kattosh showed the highest affinity for the α‐amylase enzyme, PPAR, and AMPK receptors. Results demonstrate that the affinity of Kattosh phytocompounds potentially attenuates pancreatic and kidney lesions and could be approached as an alternative antidiabetic source with further clarification.

Pharmacological insights into Chukrasia velutina bark: Experimental and computer‐aided approaches

“…A lot of medicinal plants are used in traditional medicine all over the world to reduce pain and treat hyperthermia; they can also play a vital role in counteracting oxidation and cytotoxicity in cells. 21 The The formalin-induced nociception model provides a longlasting pain with peripheral inflammation and central sensitization. 4 This protocol involves a biphasic reaction consisting of an early (neurogenic) phase and a late (inflammatory) phase.…”

“…A lot of medicinal plants are used in traditional medicine all over the world to reduce pain and treat hyperthermia; they can also play a vital role in counteracting oxidation and cytotoxicity in cells. 21 The present study was designed to investigate the anti‐nociceptive, antipyretic, antioxidant and cytotoxic effects of a methanol extract of C. velutina bark on pain stimulation. Pain is currently a major global health issue evident as headaches, cancer, inflammation, arthritis, osteoporosis and fever.…”

Upregulation of Antioxidative Gene Expression by Lasia spinosa Organic Extract Improves the Predisposing Biomarkers and Tissue Architectures in Streptozotocin-Induced Diabetic Models of Long Evans Rats

“…Following administration, meals were withdrawn for 3–4 h. The mortality and motor behavior for morbidity of the treated groups were observed for 24 h. Different parameters were carefully observed such as skin and fur, eyes, tremor, diarrhea, convulsions, salivation, mucous membranes, lethargy, respiration, behavior pattern, allergic syndromes, etc. The median fatal dose (LD 50 > 2.0 g/kg) was employed, as an effective therapeutic dose [ 13 ].…”

“…Next, STZ (60 mg/kg b.w. ), dissolved in 0.1 M citrate buffer (pH 4.5), was injected intraperitoneally to all groups, but only citrate buffer was injected to the normal control group according to Mostafavinia [ 13 , 14 ]. STZ, being a potential β-cell toxin, induces partial β-cell dysfunction, and thus, induces type 2 diabetes.…”

Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPARγ

“…We previously demonstrated that isoflavone protects from kidney injury in obese animals via PPAR-γ [14], prevents bone loss in ovariectomized diabetic animals [15], and can attenuate the effects of vulvovaginal atrophy in women [16]. Taking into account the protective function of PPAR-γ in kidney injury [17][18][19][20], the objective of this work was to evaluate the effect of ovariectomy and the consequent gonadal failure on the development of DM1induced kidney injury, as well as the contribution of soy isoflavone via PPAR-γ in this experimental animal model. Our hypothesis is that gonadal failure potentiates renal injury induced by chronic hyperglycemia and that isoflavone treatment attenuates this condition by PPAR-γ induction.…”