Increment of fructan biosynthesis in rice by transformation of 1-sst and 1-fft genes isolated from jerusalem artichoke (Helianthus tuberosus L.)

Abstract

Fructan has been found to accumulate in various tissues during periods when light levels increased carbon fixation where low temperatures reduced growth rates while photosynthesis continued. In this study, we have cloned 1-sucrose:sucrose fructosyl transferase(1-sst) and 1-fructan: fructan fructosyl transferase (1-fft, a key enzyme for the synthesis of fuctan) from Jerusalem Artichoke (Helianthus tuberosus L.). The recombinant vector with 1-sst and 1-fft has been constructed under the control of 35S promoter of KJGV-B2 vector and transgenic plants obtained by Agrobacterium tumefaciens LBA4404. PCR analysis carried out on the putative transgenic plants for amplification of the coding region of specific gene (1-sst, 1-fft), and HPT genes. Transgenic lines carrying of 1-sst and 1-fft were confirmed for integration into the rice genome using Southern blot hybridization and RT-PCR. The transgenic plants in T 2 generation were selected and expression pattern analysis revealed that 1-sst and 1-fft were stable. This analysis confirmed the presence of low-molecular-weight fructan in the seedling of the transgenic rices. Therefore, cold tolerance and carbohydrate metabolism will be possible to develop resistant plants using the transgenic rice.

Inulin-producing Genes in Gembili (Dioscorea esculenta) and Future Applications for Food Industries in Indonesia

“…Genetic modification of non-inulin-accumulating plants to synthesize inulin is another promising strategy. Examples of plants that have been used are sugar beet (Sévenier et al, 1998), tobacco (Li et al, 2007), maize (Stoop et al, 2007), rice (Kang et al, 2010), and potatoes . Inulin-producing transgenics often showed low sucrose or starch content along with stunted growth compared to wild type, probably as compensation for the extra energy and substrates need for inulin biosynthesis (van Arkel et al, 2013).…”