Increment of growth factors in mouse skin treated with non-thermal plasma

Abstract

Non-thermal plasma (NTP) has several beneficial effects, and can be applied as a novel instrument for skin treatment. Recently, many types of NTP have been developed for potential medical or clinical applications, but their direct effects on skin activation remain unclear. In this study, the effect of NTP on the alteration of mouse skin tissue was analyzed. After NTP treatment, there were no signs of tissue damage in mouse skin, whereas significant increases in epidermal thickness and dermal collagen density were detected. Furthermore, treatment with NTP increased the expression of various growth factors, including TGF-α, TGF-β, VEGF, GM-CSF, and EGF, in skin tissue. Therefore, NTP treatment on skin induces the expression of growth factors without causing damage, a phenomenon that might be directly linked to epidermal expansion and dermal tissue remodeling.

Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis

“…The most important feature of NTAP that causes various physical and chemical changes in both inorganic and organic structures is the production of ultraviolet, free radicals, ozone, and reactive oxygen and nitrogen species with the application of NTAP. Through these products, NTAP may enhance biomodification, antimicrobial, and biostimulation activities; therefore, they can be used for different purposes in dentistry, medicine, and biomedical fields [11][12][13] Nevertheless, our understanding of the role of NTAP in periodontal disease and treatment as well as of the underlying mechanisms is still limited. The aim of this study was to investigate the protective effects of NTAP on alveolar bone loss histomorphometrically and histopathologically in experimental periodontitis in rats.…”

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

“…In addition, they found β-defensins, a class of host defense peptides characterized by their antimicrobial activity and capacity for immune stimulation, to be increased. Experiments using a different type of argon-driven DBD found, in addition, an enhanced expression of cytokines and chemokines, such as TGF-β, VEGF (vascular endothelial growth factor), GM-CSF (granulocyte/macrophage colony-stimulating factor), and EGF (epidermal growth factor), along with enhanced epidermal thickness [ 82 ]. A lack of side effects, inflammation, or collagen production was also found for another DBD that moreover led to increased NO (nitric oxide) deposition into the tissues [ 76 ].…”

Modifying the Tumour Microenvironment: Challenges and Future Perspectives for Anticancer Plasma Treatments

“…Major ECM component, supports cell movement through ECM [168] Oxidized amino acids, broke H-bonds [153], and loosen COL I structure [154]. Fibroblast activation to produce COL [169] ROS induce overproduction, deposition and remodelling of COL to generate stiffer tissue ECM in tumours [104,170] Laminin (LM) Glycoprotein, important in cell differentiation, migration, and adhesion [171]. Cell-cell and cell-ECM interaction…”

“…Induced FN formation in activated fibroblasts [169] ROS oxidize FN [166]. HOCl causes FN oxidation, which modulates cell adhesion, proliferation, and mRNA expression [177,178] Elastin Entropic elastic behaviour, its stretch is limited by its association with collagen [179] Fibroblast activation in mouse skin to produce elastin [169] ROS destroy elastin network integrity, influence production of ECM proteins [180]. ONOOH induces tyrosine nitration and crosslinking in topoelastin, alters its structure, function, and changes matrix assembly [181] Adhesion to ECM…”