Incubator Analyzer is a calibration tool used to measure temperature, mattress temperature, humidity, airflow, and sound level in baby incubators. The purpose of this research is to make a tool "Design of Incu Analyzer for IOT based Baby Incubator Calibration (Chamber Temperature)” with LCD display and Thingspeak. The design of this calibration tool consists of baby incubator chamber temperature parameters consisting of 5 points, namely T1, T2, T3 , T4, T5 using the DS18B20 sensor and using the ESP32 microcontroller to take advantage of the IOT system. Utilization of IOT on ESP32 is using Wi-Fi. The ESP32 microcontroller processes data from the DS18B20 temperature sensor which is then displayed on the LCD display and Thingspeak. Testing of this tool is done by comparing incubator analyzer module with a standard measuring instrument, INCU II. In this study, the temperature parameter obtained the smallest error value of -0,059293% at T5 with a setting temperature of 36°C and the largest error value of -0,0254188% at T2 with a setting temperature of 35°C. Overall the conclusions obtained after carrying out the literature study process, and planning, it can be concluded that the tool "Incu Analyzer Design for IOT based Baby Incubator Calibration" can be used according to the plan, namely Incubator Analyzer with IOT based.