Incubation of Cyanobacteria under Dark, Anaerobic Conditions and Quantification of the Excreted Organic Acids by HPLC

Abstract

Background Euglena gracilis is a unicellular eukaryotic microalgae found in aquatic environments. It can adapt its morphology in response to various environmental stress factors such as changes in temperature, light, and ion concentrations. E. gracilis cells excrete succinate and amino acids under dark and anaerobic conditions; however, only a few studies on the effect of these conditions on the cell

Effect of pH on metabolite excretion and cell morphology of Euglena gracilis under dark, anaerobic conditions

“…Succinate was quantified by high-performance liquid chromatography as previously described with certain modifications [35]. After dark, anaerobic incubation, 1 ml of filtrates was dried with a vacuum dryer CC-105 (TOMY, Tokyo, Japan), and resuspended in 97 μL HClO 3 and 3 μL of trichloroacetic acid (Wako, Osaka, Japan).…”

Effect of pH on metabolite excretion and cell morphology of Euglena gracilis under dark, anaerobic conditions

“…Succinate was quantified by high-performance liquid chromatography as previously described with certain modifications [35]. After dark, anaerobic incubation, 1 ml of filtrates was dried with a vacuum dryer CC-105 (TOMY, Tokyo, Japan), and resuspended in 97 L HClO 3 and 3 L of trichloroacetic acid (Wako, Osaka, Japan).…”