Incubation under fluid dynamic conditions markedly improves the structural preservation in vitro of explanted skeletal muscles

Abstract

Explanted organs and tissues represent suitable experimental systems mimicking the functional and structural complexity of the living organism, with positive ethical and economic impact on research activities. However, their preservation in culture is generally limited, thus hindering their application as experimental models for biomedical research. In the present study, we investigated the potential of an innovative fluid dynamic culture system to improve the structural preservation in vitro of explanted mouse skeletal muscles (soleus). We used light and transmission electron microscopy to compare the morphological features of muscles maintained either in multiwell plates under conventional conditions or in a bioreactor mimicking the flow of physiological fluids. Our results demonstrate that fluid dynamic conditions markedly slowed the progressive structural deterioration of the muscle tissue occurring during the permanence in the culture medium, prolonging the preservation of some organelles such as mitochondria up to 48 h.

Integrated Microscopy and Metabolomics to Test an Innovative Fluid Dynamic System for Skin Explants In Vitro

“…To this aim, we have modified a bioreactor originally designed for cell culture that gave excellent results with different cellular models (Vinci et al, 2010, 2011, 2012; Iori et al, 2012; Giusti et al, 2014). The same bioreactor significantly improved the preservation of explanted skeletal muscles in comparison with conventional conditions, thus prolonging the suitability of the organ for in vitro tests (Carton et al, 2017).…”

Nanomedicine for Gene Delivery and Drug Repurposing in the Treatment of Muscular Dystrophies

“…Moreover, fluid dynamic systems may improve the organ preservation, overcoming the loss of vasculature function by mimicking the physiological flow for nutrient supply and catabolite withdrawal, thus supporting the metabolic activity of the explanted tissues [ 243 , 247 ]. Recently, Carton et al demonstrated the great benefit in terms of structural preservation obtained by maintaining explanted soleus murine muscle in a bioreactor under dynamic conditions [ 248 ]. The progressive structural deterioration of the muscle tissue was markedly slowed, prolonging its preservation up to two days.…”

Histochemistry as a versatile research toolkit in biological research, not only an applied discipline in pathology

“…[80][81][82][83][84][85] To expand the application potential of the histochemical approach and to increase the informational capacity of the histochemical evidence, novel techniques were set up [86][87][88][89][90][91][92][93][94][95] or original applications of established histochemical methods were described, using tissue and cell models from a variety of organisms (invertebrates, vertebrates or plants). [96][97][98][99][100][101][102][103][104]…”