Independent case-control study in KCNJ11 gene polymorphism with Type 2 diabetes Mellitus

Alqadri, Nada

doi:10.1016/j.sjbs.2022.01.008

Cited by 8 publications

(6 citation statements)

References 27 publications

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“…The GWAS study of human T2DM has accumulated hundreds of related gene loci, such as TCF7L2, PPARG, and KCNJ11. [14][15][16] In addition, various SNP analyses have been used in the genetic research of T2DM. [17][18][19] However, obvious ethnic and regional differences exist in the combinations of susceptibility genes due to the genetic heterogeneity of T2DM.…”

Section: Discussionmentioning

confidence: 99%

Whole‐genome sequencing study to identify candidate markers indicating susceptibility to type 2 diabetes in Bama miniature pigs

Niu

Zhao

Jia

et al. 2023

Anim Models and Exp Med

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BackgroundHundreds of single‐nucleotide polymorphism (SNP) sites have been found to be potential genetic markers of type 2 diabetes mellitus (T2DM). However, SNPs related to T2DM in minipigs have been less reported. This study aimed to screen the T2DM‐susceptible candidate SNP loci in Bama minipigs so as to improve the success rate of the minipig T2DM model.MethodsThe genomic DNAs of three Bama minipigs with T2DM, six sibling low‐susceptibility minipigs with T2DM, and three normal control minipigs were compared by whole‐genome sequencing. The T2DM Bama minipig‐specific loci were obtained, and their functions were annotated. Meanwhile, the Biomart software was used to perform homology alignment with T2DM‐related loci obtained from the human genome‐wide association study to screen candidate SNP markers for T2DM in Bama miniature pigs.ResultsWhole‐genome resequencing detected 6960 specific loci in the minipigs with T2DM, and 13 loci corresponding to 9 diabetes‐related genes were selected. Further, a set of 122 specific loci in 69 orthologous genes of human T2DM candidate genes were obtained in the pigs. Collectively, a batch of T2DM‐susceptible candidate SNP markers in Bama minipigs, covering 16 genes and 135 loci, was established.ConclusionsWhole‐genome sequencing and comparative genomics analysis of the orthologous genes in pigs that corresponded to the human T2DM‐related variant loci successfully screened out T2DM‐susceptible candidate markers in Bama miniature pigs. Using these loci to predict the susceptibility of the pigs before constructing an animal model of T2DM may help to establish an ideal animal model.

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Section: Discussionmentioning

confidence: 99%

Whole‐genome sequencing study to identify candidate markers indicating susceptibility to type 2 diabetes in Bama miniature pigs

Niu

Zhao

Jia

et al. 2023

Anim Models and Exp Med

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“…We also adopted a multiplicative sickness model and predicted disease allele frequencies of 0.25. This study needed to have at least 85% power to reject the null hypothesis with an odds ratio (OR) of 1.5 and 200 cases and 150 controls (Chehadeh et al, 2016;Alqadri, 2022). However, due to criteria selection in our study, we were only able to include 60 people with T2DM and 60 healthy controls.…”

Section: Sample Sizementioning

confidence: 99%

“…The 120 participants of both T2DM Cases and healthy controls were collected from various parts of the Countries. The inclusion and exclusion criteria of T2DM cases and controls were defined in our previous study (Alqadri, 2022). Normal glucose levels were used to select healthy controls (n=60), who had no history of diabetes are considered as inclusion criteria.…”

Section: T2dm Subjectsmentioning

confidence: 99%

“…Blood glucose levels were determined using the serum from plain tubes that had been collected and sent to a clinical chemistry laboratory for analysis. Glycated hemoglobin (Hb1Ac) levels were determined using an EDTA sample obtained from 120 subjects (Alqadri, 2022).…”

Section: Biochemical Analysismentioning

confidence: 99%

“…Both TCF7L2 (rs7903146) and KCNQ1 (rs2237892) gene polymorphisms were amplified using specific oligonucleotide sequence primers (Rattanatham et al, 2021). TCF7L2 (rs7903146) and KCNQ1 (rs2237892) gene polymorphisms were subjected to the following PCR protocol: 10 minutes of initial denaturation at 95⁰C, 40 cycles of the total reaction involving denaturation at 94⁰C for 1 minute, 1 minute of annealing temperature at 62⁰C, 1 minute of extension at 72⁰C, and a final 10 minutes of 72⁰C extension time (Alqadri, 2022). Prior to digestion, the KCNQ1 (rs2237892) and TCF7L2 (rs7903146) gene polymorphisms PCR products were amplified with 2µl of HpaII and RsaI restriction enzymes, respectively, from BioLabs in New England.…”

Section: Molecular Investigationmentioning

confidence: 99%

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Influence of KCNQ1 and TCF7L2 genes associated with the role of type 2 diabetes

Alqadri¹,

Abdelmutalab²,

Abdelhafeez³

et al. 2022

Int. j. adv. appl. sci.

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T2DM is a long-term metabolic disorder characterized by either the pancreas' inability to produce enough insulin or the body's inability to properly utilize the insulin it produces. The β-cell function and blood glucose homeostasis are two areas where TCF7L2 (Transcription factor 7 like 2) appears to be a significant candidate gene. KCNQ1 (potassium voltage-gated channel subfamily, member 1 has been discovered as a T2DM susceptibility gene in Asian populations by genome-wide association studies with rs2237892 polymorphism and an increased risk of developing T2DM. The aim of this study was to investigate the association between rs7903146 and rs2237892 SNP studies in T2DM patients. In this study, 60 T2DM cases and 60 controls were selected. Genotyping was performed for rs7903146 and rs2237892 SNPs using specific primers and restriction enzymes, then all PCR products were loaded on an agarose gel stained with ethidium bromide. The current study results confirmed rs7903146 SNP was strongly associated with genotype (OR-4.14; 95%CI:1.07-15.98; p=0.02) and allele frequencies (OR-4.60; 95%CI:1.66-12.70; p=0.001) whereas in rs2237892 SNP was not associated with any of the genotypes (OR-4.29; 95%CI:0.46-39.58; p=0.16; OR-3.21) or allele frequencies (OR-6.26; 95%CI:0.74-52.83; p=0.055). The current study results were found to be associated with global studies carried out in rs7903146 and rs2237892 SNP. The strength of this current study was to involve Saudi nationalities and we have screened rs7903146 and rs2237892 SNPs which plays a major role in T2DM. Involving 60 T2DM cases/60 controls was the major limitation of this study. Missing validation through Sanger sequencing analysis was one of the limitations of this study. In conclusion, the current study results confirmed rs7903146 SNP was strongly associated with T2DM and rs2237892 SNP was not associated with T2DM patients.

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Potassium inwardly-rectifying channel subfamily J member 11 (KCNJ11) gene polymorphism in Egyptian type 2 diabetic patients: a single-center study

Abdelazem,

Gaber,

Hussein

et al. 2024

Mol Biol Rep

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Independent case-control study in KCNJ11 gene polymorphism with Type 2 diabetes Mellitus

Cited by 8 publications

References 27 publications

Whole‐genome sequencing study to identify candidate markers indicating susceptibility to type 2 diabetes in Bama miniature pigs

Whole‐genome sequencing study to identify candidate markers indicating susceptibility to type 2 diabetes in Bama miniature pigs

Influence of KCNQ1 and TCF7L2 genes associated with the role of type 2 diabetes

Potassium inwardly-rectifying channel subfamily J member 11 (KCNJ11) gene polymorphism in Egyptian type 2 diabetic patients: a single-center study

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