2011
DOI: 10.1111/j.1365-2958.2011.07757.x
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Independent evolutionary origins of functional polyamine biosynthetic enzyme fusions catalysingde novodiamine to triamine formation

Abstract: Summary We have identified gene fusions of polyamine biosynthetic enzymes S-adenosylmethionine decarboxylase (AdoMetDC, speD) and aminopropyltransferase (speE) orthologues in diverse bacterial phyla. Both domains are functionally active and we demonstrate the novel de novo synthesis of the triamine spermidine from the diamine putrescine by fusion enzymes from β-proteobacterium Delftia acidovorans and δ-proteobacterium Syntrophus aciditrophicus, in a ΔspeDE gene deletion strain of Salmonella enterica sv. Typhim… Show more

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Cited by 20 publications
(18 citation statements)
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“…In Pseudomonas aeruginosa , Campylobacter jejuni, and Agrobacterium tumefaciens , spermidine is essential for planktonic growth (1113). In contrast, polyamine auxotrophic strains of Escherichia coli grow for multiple subcultures under aerobic conditions, albeit with a 40% reduction in growth rate, and a similar reduction in growth rate was observed in polyamine auxotrophic strains of Yersinia pestis , Salmonella enterica serovar Typhimurium, and Vibrio cholerae (1416). Spermidine auxotrophic strains of the Gram-positive species Bacillus subtilis and Streptococcus pneumoniae exhibit normal planktonic growth in the absence of spermidine (1719).…”
Section: Introductionmentioning
confidence: 78%
“…In Pseudomonas aeruginosa , Campylobacter jejuni, and Agrobacterium tumefaciens , spermidine is essential for planktonic growth (1113). In contrast, polyamine auxotrophic strains of Escherichia coli grow for multiple subcultures under aerobic conditions, albeit with a 40% reduction in growth rate, and a similar reduction in growth rate was observed in polyamine auxotrophic strains of Yersinia pestis , Salmonella enterica serovar Typhimurium, and Vibrio cholerae (1416). Spermidine auxotrophic strains of the Gram-positive species Bacillus subtilis and Streptococcus pneumoniae exhibit normal planktonic growth in the absence of spermidine (1719).…”
Section: Introductionmentioning
confidence: 78%
“…1B and 1C). We have shown recently that some of these bacterial AdoMetDC-aminopropyltransferase fusions proteins are able to synthesize de novo spermidine from putrescine and sym-norspermidine from 1,3-diaminopropane [28]. This is a strong indication that the diatom AdoMetDC-aminopropyltransferase fusion proteins are likely to be functional polyamine biosynthetic modules, and being much bigger, the diatom fusion proteins may be capable of producing iteratively elongated LCPA within their active sites.…”
Section: Molecular Machines For Long Chain Polyamine Biosynthesismentioning
confidence: 99%
“…Spermidine depletion results in approximately 50% reduction in growth rate in γ‐proteobacteria Escherichia coli (Chattopadhyay et al, ), Yersinia pestis (Patel et al, ) and Salmonella enterica sv. Typhimurium (Green et al, ). Putrescine is essential for growth of β‐proteobacterium Ralstonia solanacearum (Lowe‐Power et al, ).…”
Section: Introductionmentioning
confidence: 99%
“…Not shown is the variant S-adenosylmethionine-dependent pathway of Thermus thermophilus and Thermococcus kodakarensis, which uses homologues of agmatinase and spermidine synthase to synthesize spermidine from agmatine via aminopropylagmatine (Ohnuma et al, 2005 (Chattopadhyay et al, 2009), Yersinia pestis (Patel et al, 2006) and Salmonella enterica sv. Typhimurium (Green et al, 2011). Putrescine is essential for growth of β-proteobacterium Ralstonia solanacearum (Lowe-Power et al, 2018).…”
Section: Introductionmentioning
confidence: 99%