Independent prognostic genes and mechanism investigation for colon cancer

Li, Chunsheng; Shen, Zhen; Zhou, Yangyang; Yu, Wei

doi:10.1186/s40659-018-0158-7

Cited by 25 publications

(23 citation statements)

References 45 publications

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“…However, even with this issue is worthy to study differences in NT and tumours samples. Recent works that used TCGA and GEO publicly available datasets also found relevant results despite the difference in sample sizes [26–28]. Taking together, these results indicate a possible role of TULP3 in the diagnosis of CRC, however, additional studies are needed to confirm these preliminary findings.…”

Section: Discussionmentioning

confidence: 66%

TULP3: A potential biomarker in colorectal cancer?

2019

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Colorectal cancer (CRC) is the second most common cancer in women and the third most common cancer in men globally. The identification of differentially expressed genes associated to patient’s clinical data may represent a useful approach to find important genes in CRC carcinogenesis. Previously, the TULP3 transcription factor was identified as a possible prognostic biomarker in pancreatic ductal adenocarcinoma. Considering that pancreatic and colorectal tissues have the same embryonic origin, we investigated the profile of TULP3 expression in CRC hypothesizing that it may have a role in its development. We comparatively analysed TULP3 gene expression in CRC and normal adjacent colonic tissue and assessed association of expression profiles with survival and clinicopathological information, using publicly available datasets. TULP3 expression levels were increased in CRC when compared to the adjacent non-tumoral tissue. In addition, higher TULP3 gene expression was associated to lymphatic and vascular invasion in colon adenocarcinoma (COAD) and rectum adenocarcinoma (READ), respectively. In summary, our results point to a possible role of TULP3 as a diagnostic and prognostic biomarker in CRC. Additional studies are necessary to confirm these preliminary findings.

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Section: Discussionmentioning

confidence: 66%

TULP3: A potential biomarker in colorectal cancer?

2019

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“…WBSCR28 had not been well studied in human tumor, but it was repressed by androgen receptor in prostate cancer [69]. SFTA2 was identified as a potential disease-free survival prognostic gene in colon cancer and one of the potential biomarkers for distinguishing between lung adenocarcinoma and squamous cell carcinoma [70,71]. MYH2 was confirmed significantly changed in hepatocellular carcinoma and highly expressed in the origin of squamous cell carcinoma in the lungs of patients with previous head and neck malignancies [72,73].…”

Section: Discussionmentioning

confidence: 99%

Identification of Hub Genes Related to Carcinogenesis and Prognosis in Colorectal Cancer Based on Integrated Bioinformatics

Gong

Kao

Zhang

et al. 2020

Mediators of Inflammation

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The high mortality of colorectal cancer (CRC) patients and the limitations of conventional tumor-node-metastasis (TNM) stage emphasized the necessity of exploring hub genes closely related to carcinogenesis and prognosis in CRC. The study is aimed at identifying hub genes associated with carcinogenesis and prognosis for CRC. We identified and validated 212 differentially expressed genes (DEGs) from six Gene Expression Omnibus (GEO) datasets and the Cancer Genome Atlas (TCGA) database. We investigated functional enrichment analysis for DEGs. The protein-protein interaction (PPI) network was constructed, and hub modules and genes in CRC carcinogenesis were extracted. A prognostic signature was developed and validated based on Cox proportional hazards regression analysis. The DEGs mainly regulated biological processes covering response to stimulus, metabolic process, and affected molecular functions containing protein binding and catalytic activity. The DEGs played important roles in CRC-related pathways involving in preneoplastic lesions, carcinogenesis, metastasis, and poor prognosis. Hub genes closely related to CRC carcinogenesis were extracted including six genes in model 1 (CXCL1, CXCL3, CXCL8, CXCL11, NMU, and PPBP) and two genes and Metallothioneins (MTs) in model 2 (SLC26A3 and SLC30A10). Among them, CXCL8 was also related to prognosis. An eight-gene signature was proposed comprising AMH, WBSCR28, SFTA2, MYH2, POU4F1, SIX4, PGPEP1L, and PAX5. The study identified hub genes in CRC carcinogenesis and proposed an eight-gene signature with good reproducibility and robustness at the molecular level for CRC, which might provide directive significance for treatment selection and survival prediction.

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“…The current treatments for CC include surgery, radiation therapy, chemotherapy, and targeted therapy (Li, Shen, Zhou, & Yu, 2018). People with early-stage CC may be cured via surgery, whereas CC patients at the advanced stages treated with the combined chemotherapy are usually not curable due to drug resistance (Ma et al, 2012;Yu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Forkhead box O3 promotes colon cancer proliferation and drug resistance by activating MDR1 expression

Gao

Liu

et al. 2019

Molec Gen & Gen Med

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Background Globally, colon cancer (CC) is the third reason of tumor‐related deaths. Previous reports indicate that Forkhead box O3 (FOXO3) is involved in the development of various tumors and may have different effects depending upon the types of tumors. Hence, this study was to examine the effects of FOXO3 on CC cells and uncover the possible mechanisms. Methods MTT and cell count assay were applied to analyze the viability of transfected CC cells. rVista, dual luciferase reporter assay, and chromatin immunoprecipitation assay were used to identify the downstream target of FOXO3 in HCT116 cells. The mRNA and protein abundance of FOXO3 and MDR1 were determined by quantitative PCR and Western blot, respectively. Results Forkhead box O3 stimulated the proliferation of both HCT116 and DLD1 cells. Moreover, FOXO3 overexpression inhibited doxorubicin sensitivity of HCT116 cells, while the knockout of FOXO3 by FOXO3 shRNA restored the doxorubicin sensitivity in doxorubicin‐resistant HCT116 DR cells. Next, we found that FOXO3 directly bound to the promoter of MDR1 and enhanced MDR1 expression in HCT116 cells. MDR1 overexpression enhanced the viability and doxorubicin resistance of CC cells. Besides, MDR1 overexpression plasmid significantly abrogated the decrease in cell proliferation and resistance of HCT116 cells to doxorubicin caused by FOXO3 knockout. Conclusion Forkhead box O3 exhibited promotive effects on the proliferation and doxorubicin resistance in CC cells via targeting MDR1.

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Independent prognostic genes and mechanism investigation for colon cancer

Cited by 25 publications

References 45 publications

TULP3: A potential biomarker in colorectal cancer?

TULP3: A potential biomarker in colorectal cancer?

Identification of Hub Genes Related to Carcinogenesis and Prognosis in Colorectal Cancer Based on Integrated Bioinformatics

Forkhead box O3 promotes colon cancer proliferation and drug resistance by activating MDR1 expression

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