Independent regulation of pyruvate kinase expression by cyclic AMP and prostaglandin F2α in mouse mastocytoma cells

Ngo, Julie-Lani; Ibsen, Kenneth H.

doi:10.1016/0003-9861(89)90128-8

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…The increase in activity is dependent upon the presence of PGFza, FGF and insulin (Table 11). PGFza increases the rate of pyruvate kinase-K synthesis and pyruvate kinase mRNA levels in mouse mastocytoma cells (Ngo and Ibsen, 1989), suggesting that this hormone may also regulate pyruvate kinase activity at the transcriptional level in astrocytes. Although no role for insulin or FGF has been associated with the regulation of levels, it is also conceivable that the insulin is acting on somatomedin receptors through the same mechanism as FGF.…”

Section: Effect Of Cdm On Specific Activitymentioning

confidence: 99%

Expression of pyruvate kinase in astrocytes induced to differentiate in vitro

Ngo

Ibsen

1989

J of Neuroscience Research

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Astrocytes maintained in a chemically defined media undergo differentiation and a parallel increase in pyruvate kinase specific activity. These changes are accompanied by a shift in the isoelectrofocusing pattern, but not by expression of pyruvate kinase M4, the characteristic adult rat brain isozyme. Thus, this chemically defined media lacks a substance required to induce pyruvate kinase M synthesis and this function can be uncoupled from other aspects of cellular differentiation. The uncoupling of pyruvate kinase maturation from cellular differentiation and the observation of only a single, 2.3 kilobase, pyruvate kinase mRNA molecule at different stages of the postnatal development of rat brain support the concept that the K- to M-isoform transformation is a post-transcriptional event. The effect of the individual components of this chemically defined medium on pyruvate kinase specific activity was studied by eliminating one component at a time. The increase in activity was found to be completely dependent upon fibroblastic growth factor and prostaglandin F2 alpha and was partially dependent on the simultaneous presence of insulin.

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Section: Effect Of Cdm On Specific Activitymentioning

confidence: 99%

Expression of pyruvate kinase in astrocytes induced to differentiate in vitro

Ngo

Ibsen

1989

J of Neuroscience Research

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Carbohydrate Metabolism and Neoplasia: New Perspectives for Diagnosis and Therapy

Eigenbrodt¹,

Gerbracht²,

Mazurek³

et al. 1994

Biochemical and Molecular Aspects of Selected Cancers

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Molecular approaches to the manipulation of carbon allocation in plants

Blakeley¹,

Dennis²

1993

Can. J. Bot.

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1993. Molecular approaches to the manipulation of carbon allocation in plants. Can. J. Bot. 71: 765-778.In plants, sucrose is the end product of photosynthesis and is converted to a wide variety of storage compounds in tissues such as seeds and tubers. The allocation of carbon from sucrose to the various metabolic pathways leading to these products will determine the quantity of each synthesized in the respective storage organs. If the level of the enzymes involved in the allocation of carbon could be changed by genetic manipulation, it is probable that the relative yields of the various storage products can also be altered. The initial breakdown of sucrose occurs in the cytosol of the cell. Many biosynthetic pathways, however, including those involved in the synthesis of storage products such as fatty acids, starch, and amino acids, occur in the plastid. The distribution of carbon substrates for these processes will be determined, to a large extent, by the flux of carbon through the glycolytic pathways found in both the cytosolic and plastid compartments. This article will discuss the importance and consequences of compartmentation, review the extent of our understanding of glycolysis and other enzymes and pathways regulating carbon allocation, and will speculate on the potential for the genetic manipulation of these pathways. BLAKELEY, S.D., et DENNIS, D.T. 1993. Molecular approaches to the manipulation of carbon allocation in plants. Can.J. Bot. 71 : 765-778. Chez les plantes, le sucrose est le dernier produit de la photosynthkse qui est par la suite transform6 en une gamme de substances de rtserve dans les tissus des graines et des tubercules, B titre d'exemple. La distribution du carbone sous forme de sucrose dans les diffkrents sentiers mitaboliques conduisant B ces produits, dCterminera la quantitC de ceux-~i synthCtisCs dans les organes de reserves respectifs. Si on peut changer, par manipulation gCnCtique, les teneurs des enzymes impliquees dans la distribution du carbone, il est probable qu'on modifiera tgalement les rendements relatifs de ces diverses substances de rtserve. L'hydrolyse du sucrose dCbute dans le cytosol de la cellule. Cependant, plusieurs sentiers de biosynthbse, y compris ceux qui sont impliquCs dans la synthbse des substances de reserve telles que les acides gras, l'amidon, et les acides aminks, sont localisCs dans les plastes. La distribution des substrats carbones nCcessaires pour ces diffkrents processus est dCterminCe, pour I'essentiel, par le flux de carbone dans les sentiers glycolytiques qui se trouvent dans les compartiments cytosoliques aussi bien que plastidiques. Dans leur article, les auteurs discutent l'importance et les consCquences de la compartimentation, ils revoient l'ensemble de notre comprChension de la glycolyse ainsi que des autres enzymes et sentiers impliquCs dans la regulation de la distribution du carbone et ils Cvoquent ce que la manipulation gCnCtique de ces sentiers pourrait apporter de nouveau.

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Independent regulation of pyruvate kinase expression by cyclic AMP and prostaglandin F2α in mouse mastocytoma cells

Cited by 3 publications

References 45 publications

Expression of pyruvate kinase in astrocytes induced to differentiate in vitro

Expression of pyruvate kinase in astrocytes induced to differentiate in vitro

Carbohydrate Metabolism and Neoplasia: New Perspectives for Diagnosis and Therapy

Molecular approaches to the manipulation of carbon allocation in plants

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