India and Africa have experienced the rapid expansion of bilateral trade during the last two decades. The India–Africa trade is understudied in general and in the agriculture sector. Very few considerable efforts have been made to study the agro-trade restrictions between both the economies. Therefore, the purpose of this paper is to empirically identify the recent evidence of non-tariff measures (NTMs) imposed on the agro-products between India and Africa, which also work as non-tariff barriers. It is perceived that India being one of the frequent users of NTMs in the world poses many challenges for its trading partners. Based on the Revealed Trade Barrier (RTB) index, using 28 agro-products (HS-4 digit level), this paper assesses bilateral agro-trade barriers. The frequency index ( Fi) and coverage ratio ( Cr) were used to analyse the complex nature of NTMs. The study findings show that both trading partners imposed a wide range of NTMs on each other’s agro-products, which resulted in the discriminatory effects on trade. Comparatively, India has imposed the lesser number of NTMs on Africa’s agro-products. Despite the recent bilateral trade agreements between both the economies, trade barriers were frequently noticed. The paper suggests applying strategic trade policies and reduction of NTMs along with harmonisation of standards to flourish the bilateral agro-trade.