Acquisition and analysis of hydrological series are essential activities for hydrological studies in watersheds. However, they require time and are usually complex and susceptible to human errors. In order to minimize these problems, computational tools are often used for hydrological analysis, although there are few of them in Brazil. This technical note aims to provide a structured document with information on the motivation, development, conception, main functionalities, and applications of the System of Hydrological Data Acquisition and Analysis (SYHDA). SYHDA is a software intended for acquisition and analysis of hydrological data (rainfall and streamflow) and was fully idealized to enable the user to deal with the country’s leading hydrology databases. It has several modules that include analysis by descriptive statistics and graphical tools, seasonality analysis of streamflows, non-parametric tests, and at-site and regional probabilistic modeling. SYHDA has been used in numerous scientific studies, which give grounds to affirm that it demonstrates a great potential to be used in both everyday and complex activities demanded by the scientific and technical community of hydrology and related areas.