Indigenous hydrocarbon-utilizing bacterioflora in oil-polluted habitats in Kuwait, two decades after the greatest man-made oil spill

Al-Awadhi, H.; Al-Mailem, Dina M.; Dashti, Narjes; Khanafer, Majida; Radwan, S. S.

doi:10.1007/s00203-012-0800-7

Cited by 53 publications

(46 citation statements)

References 31 publications

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“…Several of the genera recorded in Tables 1, 2 and Online Resources 4, 5 comprise mesophilic species cited in the literature as hydrocarbon utilizers. These include the genera Arthrobacter (Radwan et al 1996;Al-Awadhi et al 2012), Bacillus (Lei et al 2006;Feitkenhauer et al 2003), Fig. 4 Effect of Ca 2+ -concentration on growth (left graphs) and crude oil consumption (right) at 30 (broken lines) and 50 °C (solid) by selected isolates Brevibacillus (Hadad et al 2005), Geobacillus (Poli et al 2012), several haloarchaea (Al-Mailem et al 2010, 2014, Marinobacter (Shieh et al 2003), Mycobacterium and others.…”

Section: Bacterial Community Structures In the Freshly Collected Soilmentioning

confidence: 98%

Moderately thermophilic, hydrocarbonoclastic bacterial communities in Kuwaiti desert soil: enhanced activity via Ca2+ and dipicolinic acid amendment

2015

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Pristine and oil-contaminated desert soil samples from Kuwait harbored between 10 and 100 cells g(-1) of hydrocarbonoclastic bacteria capable of growth at 50 °C. Enrichment by incubation of moistened soils for 6 months at 50 °C raised those numbers to the magnitude of 10(3) cells g(-1). Most of these organisms were moderately thermophilic and belonged to the genus Bacillus; they grew at 40-50 °C better than at 30 °C. Species belonging to the genera Amycolatopsis, Chelativorans, Isoptericola, Nocardia, Aeribacillus, Aneurinibacillus, Brevibacillus, Geobacillus, Kocuria, Marinobacter and Paenibacillus were also found. This microbial diversity indicates a good potential for hydrocarbon removal in soil at high temperature. Analysis of the same desert soil samples by a culture-independent method (combined, DGGE and 16S rDNA sequencing) revealed dramatically different lists of microorganisms, many of which had been recorded as hydrocarbonoclastic. Many species were more frequent in the oil contaminated than in the pristine soil samples, which may reflect their hydrocarbonoclastic activity in situ. The growth and hydrocarbon consumption potential of all tested isolates were dramatically enhanced by amendment of the cultures with Ca(2+) (up to 2.5 M CaSO4). This enhanced effect was even amplified when in addition 8 % w/v dipicolinic acid was amended. These novel findings are useful in suggesting biotechnologies for waste hydrocarbon remediation at moderately high temperature.

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Section: Bacterial Community Structures In the Freshly Collected Soilmentioning

confidence: 98%

Moderately thermophilic, hydrocarbonoclastic bacterial communities in Kuwaiti desert soil: enhanced activity via Ca2+ and dipicolinic acid amendment

2015

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“…Also the four genera of phyllospheric bacteria analyzed by earlier researchers (Yutthammo et al 2010) using the cultureindependent approach, namely Acinetobacter (Lal and Khanna 1996;Al-Awadhi et al 2012b),…”

Section: Measurements By Earlier Investigators Showed That Plant Leavmentioning

confidence: 99%

Bioremediation of Atmospheric Hydrocarbons via Bacteria Naturally Associated with Leaves of Higher Plants

Ali

Al-Awadhi

Dashti

et al. 2015

International Journal of Phytoremediation

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Bacteria associated with leaves of sixteen cultivated and wild plant species from all over Kuwait were analyzed by a culture-independent approach. This technique depended on partial sequencing of 16S rDNA regions in total genomic DNA from the bacterial consortia and comparing the resulting sequences with those in the GenBank database. To release bacterial cells from leaves, tough methods such as sonication co-released too much leaf chloroplasts whose DNA interfered with the bacterial DNA. A more satisfactory bacterial release with a minimum of chloroplast co-release was done by gently rubbing the leaf surfaces with soft tooth brushes in phosphate buffer. The leaves of all plant species harbored on their surfaces bacterial communities predominated by hydrocarbonoclastic (hydrocarbon-utilizing) bacterial genera. Leaves of 6 representative plants brought about in the laboratory effective removal of volatile hydrocarbons in sealed microcosms. Each individual plant species had a unique bacterial community structure. Collectively, the phyllospheric microflora on the studied plants comprised the genera Flavobacterium, Halomonas, Arthrobacter, Marinobacter, Neisseria, Ralstonia, Ochrobactrum. Exiguobacterium, Planomicrobium, Propionibacterium, Kocuria, Rhodococcus and Stenotrophomonas. This community structure was dramatically different from the structure we determined earlier for the same plants using the culture-dependent approach, although in both cases, hydrocarbonoclastic bacteria were frequent.

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“…In this work, the results obtained by molecular analysis were compared with the results obtained by culture-dependent analysis of a previous study [113]. In contrast to the culture-dependent technique, primers used in the molecular analysis preferentially amplified the 16S rDNA of hydrocarbonoclastic bacteria in the total environmental genomic DNA of all the studied samples.…”

Section: Culture Dependent and Independent Isolation Of Marine Micmentioning

confidence: 99%

Emerging Strategies and Integrated Systems Microbiology Technologies for Biodiscovery of Marine Bioactive Compounds

et al. 2014

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Marine microorganisms continue to be a source of structurally and biologically novel compounds with potential use in the biotechnology industry. The unique physiochemical properties of the marine environment (such as pH, pressure, temperature, osmolarity) and uncommon functional groups (such as isonitrile, dichloroimine, isocyanate, and halogenated functional groups) are frequently found in marine metabolites. These facts have resulted in the production of bioactive substances with different properties than those found in terrestrial habitats. In fact, the marine environment contains a relatively untapped reservoir of bioactivity. Recent advances in genomics, metagenomics, proteomics, combinatorial biosynthesis, synthetic biology, screening methods, expression systems, bioinformatics, and the ever increasing availability of sequenced genomes provides us with more opportunities than ever in the discovery of novel bioactive compounds and biocatalysts. The combination of these advanced techniques with traditional techniques, together with the use of dereplication strategies to eliminate known compounds, provides a powerful tool in the discovery of novel marine bioactive compounds. This review outlines and discusses the emerging strategies for the biodiscovery of these bioactive compounds.

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Indigenous hydrocarbon-utilizing bacterioflora in oil-polluted habitats in Kuwait, two decades after the greatest man-made oil spill

Cited by 53 publications

References 31 publications

Moderately thermophilic, hydrocarbonoclastic bacterial communities in Kuwaiti desert soil: enhanced activity via Ca2+ and dipicolinic acid amendment

Moderately thermophilic, hydrocarbonoclastic bacterial communities in Kuwaiti desert soil: enhanced activity via Ca2+ and dipicolinic acid amendment

Bioremediation of Atmospheric Hydrocarbons via Bacteria Naturally Associated with Leaves of Higher Plants

Emerging Strategies and Integrated Systems Microbiology Technologies for Biodiscovery of Marine Bioactive Compounds

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