Indigenous irrigants as potent antimicrobials in endodontic treatment: An in vitro  study

Satti, Parvathi; Kakarla, Pranitha; Avula, Sai Sankar Jogendra; Muppa, Radhika; Rompicharla, Sri Vishnu Kiran; Biswas, Swati

doi:10.4103/jisppd.jisppd_112_19

Cited by 11 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, agar well-diffusion method was used in the present study as it depends on the diffusion of the tested material to such an extent that growth of the added microorganism is prevented entirely in a zone around the hole containing the test material. 39 Data from the present study exhibited wide variations in their effectiveness against two tested micro-organisms i.e. S.mutans and L.plantarum.…”

Section: Discussionmentioning

confidence: 66%

Anti microbial effect of prebiotic containing tooth paste against streptococcus mutans and lactobacillus: An invitro evaluation

R¹,

Peethambar

Konde³

et al. 2022

IJMR

View full text Add to dashboard Cite

: To evaluate the antimicrobial efficacy of three commercially available toothpastes which are non-fluoridated, fluoridated and the one containing prebiotic ingredient on s and .: Antimicrobial activities of prebiotic, fluoridated, non-fluoridated toothpastes were assessed at different concentrations (100ug, 200ug, 300ug and 400ug). Since prebiotic toothpaste had other proven antimicrobial agents, 100% inulin extract from chicory (prebiotic) was assessed for its antimicrobial efficacy at varying concentrations. Antimicrobial property was evaluated by determining the zone of inhibition using agar well diffusion method on Luria Bertani (LB) broth media for and MRS broth media forL.plantarum. 100ul of inoculums of S.mutans and L.plantarum was poured on to the agar plates respectively. Five wells measuring 0.5cm was made, dentifrice at varying concentration i.e; 100ug, 200ug, 300ug and 400ug was loaded into respective wells. 50ul of DMSO was loaded in the well at the centre as control and incubated at 37°C for 24hrs. : All the three tested toothpastes had significant antimicrobial activity against S.mutans and L.plantarum. The highest antimicrobial activity was exhibited by toothpaste containing prebiotic ingredient with mean zone of inhibition of (18.5+/-0.5) and (20.0+/-1) at 400ug against S.mutans and L.plantarum. There was no significant difference in antimicrobial activity of fluoridated and non-fluoridated toothpastes against S.mutans at 400ug (P=1), but there was a significant difference for L.plantarum at 400ug (P<0.001). Antimicrobial activity increased at higher concentrations for all the three tested toothpastes and for 100% Inulin extract. : All the three tested toothpaste were effective against S.mutans and L.plantarum at varying concentrations, but toothpaste containing prebiotic ingredient was most effective in inhibition of both the micro-organisms.

show abstract

Section: Discussionmentioning

confidence: 66%

Anti microbial effect of prebiotic containing tooth paste against streptococcus mutans and lactobacillus: An invitro evaluation

R¹,

Peethambar

Konde³

et al. 2022

IJMR

View full text Add to dashboard Cite

show abstract

“…citrifolia juice (MCJ) to have antimicrobial efficacy equivalent to that of 1% NaOCl and also Triphala in DMSO comparable to that of 2.5% and 5% NaOCl. [ 32 33 34 35 36 ] Although in studies by Chaitanya et al . [ 37 ] 3% NaOCl scored better than 6% MCJ and Saxena et al .…”

Section: Resultsmentioning

confidence: 99%

“…[ 36 39 ] Satti et al . [ 32 ] demonstrated higher antimicrobial efficacy of aqueous extract Triphala as compared 3% NaOCl whereas a study by Saxena et al . [ 38 ] revealed 2.5% NaOCl to be more effective than ethanolic extract of Triphala .…”

Section: Discussionmentioning

confidence: 96%

Azadirachta indica A. juss, Morinda citrifolia L. and Triphala as herbal endodontic irrigants: A scoping review

Agnihotri

Jhamb

Shrama

et al. 2020

AYU

View full text Add to dashboard Cite

Background: The success of the root canal treatment depends on the complete elimination of the microflora, biofilms and smear layer from the pulp space. A wide variety of chemical endodontic irrigants are available to achieve disinfection and thorough debridement besides mechanical means. However, detrimental properties such as allergic potential, cytotoxicity, antimicrobial resistance and safety concerns have intrigued researchers over the years to look for safer options. Aim: The review is aimed at providing comprehensive information of the studies evaluating the efficacy of Azadirachta indica A. juss ( A. indica ), Morinda citrifolia L. ( M. citrifolia ) and Triphala (fruits of Emblica officinalis Gaertn., Terminalia chebula (Gaertn.) Roxb. and Terminalia belerica Retz.) as herbal endodontic irrigants. Materials and methods: The literature review was conducted using indexed databases (PubMed, Google Scholar, Cochrane) electronically for publications in peer-reviewed journals for relevant articles evaluating the efficacy of A. indica, Triphala, M. citrifolia as endodontic irrigant from the year 1985-March 2020. Results: A total of 58 studies were identified for full text reviewing after duplicate removal and screening title and abstracts. A total of 32 studies were included and processed for data extractions. Conclusion: Various in-vitro/in-vivo studies utilizing these herbal irrigants have documented promising results and hold the potential to replace chemical endodontic irrigants in routine practice but more preclinical and clinical trials are needed to substantiate these results before they can conclusively be recommended as intracanal irrigating solutions.

show abstract

“…The text results are shown in Table 1 . The methyl-thiazol-tetrazolium (MTT) assay, a tetrazolium salt-based colorimetric test, was the test most commonly used to assess cytotoxicity (55.1 % of the papers) [ [15] , [16] , [17] , 23 ], [ [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] ], [ [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] , [67] , [68] ]. This assay measures cell viability based on the reduction of tetrazolium salt to water-insoluble formazan crystals by dehydrogenases of mitochondria of living cells [ 69 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cytotoxicity tests are conducted using primary cell cultures [ 22 , 24 , 38 , 43 , 44 , 47 , 49 , 54 , 60 , 65 , 67 , [71] , [72] , [73] , 75 , [77] , [78] , [79] , [81] , [82] , [83] , 86 , 100 , 105 , 106 , 108 ], permanent cell lines [ 15 , 17 ], [ 23 , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] ], [ [39] , [40] , [41] ], [ 45 , 46 , 48 ], [ [51] , [52] , [53] ], [ [55] , [56] , [57] , [58] , [59] ], [ [61] , [62] , [63] , [64] ], [ 68 , 70 , 74 , 85 , 88 , 89 ], [ [93] , …”

Section: Introductionunclassified

Research methods assessing sodium hypochlorite cytotoxicity: A scoping review

Coaguila-Llerena,

Raphael da Silva,

Faria

2024

Heliyon

View full text Add to dashboard Cite

Indigenous irrigants as potent antimicrobials in endodontic treatment: An in vitro study

Cited by 11 publications

References 17 publications

Anti microbial effect of prebiotic containing tooth paste against streptococcus mutans and lactobacillus: An invitro evaluation

Anti microbial effect of prebiotic containing tooth paste against streptococcus mutans and lactobacillus: An invitro evaluation

Azadirachta indica A. juss, Morinda citrifolia L. and Triphala as herbal endodontic irrigants: A scoping review

Research methods assessing sodium hypochlorite cytotoxicity: A scoping review

Contact Info

Product

Resources

About