Indigenous traditional medicine: in vitro anti-giardial activity of plants used in the treatment of diarrhea

Brandelli, Clara Lia Costa; Giordani, Raquel Brandt; Carli, Geraldo Attílio De; Tasca, Tiana

doi:10.1007/s00436-009-1330-3

Cited by 48 publications

(30 citation statements)

References 30 publications

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“…Its aerial parts present many pharmacological properties, including anti-giardial (Brandelli et al, 2009), antimicrobial (Holetz et al, 2002;Coelho de Souza et al, 2004), and toxicity in fish hepatopancreas (Fiuza et al, 2009). So far, no reports have been found of antitumor activity or lethality in brine shrimp bioassay.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine

et al. 2012

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The brine shrimp (Artemia salina Leach) lethality bioassay offers an advantage in standardization and quality control of botanical products. This test is well correlated with antitumor activity (cytotoxicity) and can be used to monitor the activity of bioactive natural products. This paper reports the bioactivity of ethanol extracts from seven medicinal plants from the Northeast of Brazil (Acmella uliginosa, Ageratum conyzoides, Eugenia uniflora, Plectranthus neochilus, Moringa oleifera, Justicia pectoralis and Equisetum sp.) against Artemia salina. Biological activity was evaluated for extracts at 1, 10, 100, and 1000 µg/mL in triplicate, and the mean lethal concentration values (LC 50 ) were obtained by probit analysis. The species Acmella uliginosa showed the highest bioactivity, and its flower extract was more active than its leaf extract. Keywords

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Section: Discussionmentioning

confidence: 99%

Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine

et al. 2012

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“…After centrifugation at 600 rpm for 10min at 4 °C cysts concentrated at the 0.85-0.4M sucrose interface were collected and washed again. Purified cysts were resuspended in distilled water and stored at 4 °C for a maximum of 3 ysprior to use (11,29).…”

Section: Methodsmentioning

confidence: 99%

“…Initial experiments demonstrated that survival of most of the primary cultures could be favorably affected by the addition of bile and by increasing the serum component to 20% (26). Therefore the excystation medium used consisted of filter-sterilized TYI-S-33 culture medium, with bovine bile added (11,27), supplemented with 20% heat inactivated fetal calf serum. Screw capped borosilicate glass culture tubes containing 8 ml of the medium, supplemented with penicillin (500 iu/ml) and streptomycin (500 ug/ml), and were incubated at 37 °C in a slant.…”

Section: Excystation Of G Lamblia Cysts and Culture Methodsmentioning

confidence: 99%

“…Children are more frequently affected than adults, although all ages are may display symptoms ranging from mild diarrhea, Flatulence, anorexia, crampy abdominal pains, and epigastric tenderness to steaorrhea and full blow malabsorption syndrome and extra intestinal symptoms, such as fever, maculopapular rash, pulmonary infiltrates, lympha denopathy, polyarthritis and urticarial (2-3). For the treatment of Giardiasis Metronidazole as the first choice and other drugs; Tinidazole, Albandazole, Furazolidone are also used (8)(9)(10)(11). Undesirable side effects and failures in treatment and drug resistance are frequently reported (5)(6)(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

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Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro

Gholami¹,

Azadbakht²,

Hezarjaribi³

et al. 2014

Res Mol Med (RMM)

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Background: Giardiasis is a one of the most prevalent intestinal parasitic diseases in human, treatment of this disease through medicinal plants is very important since parasite resistance to chemical drugs exists. Thus, in this study, the in vitro anti-giardial activity of chloroformic extract of Tanacetum parthenium and Artemisia annua on cyst and trophozoite of Giardia lamblia were separately investigated. Materials and Methods:In this experimental study chloroformic extracts of Artemisia annua and Tanacetum parthenium on cyst and trophozoite of G. lamblia in vitro were prepared in 1, 10, 50 and 100 mg∕ml concentrations for 5, 10, 30, 60 and 180 min. Purified cysts were used for encystations and culture in TYI-S-33 medium. Then, 2 ml of each solution was placed in test tubes, to which 10,000 washed cysts and trophozoites were added. The contents of the tubes were gently mixed and incubated. The percentages of dead parasites were determined by counting 500 cysts. Non treated parasites were considered a control group in each experiment and the viability of the parasites checked with Eeosin staining and statistical analysis were done. Results:The results showed that chloroformic extracts of A. annua at 100 mg/ml concentration affected on Gardia cyst 86% and 100% trophozoite after 1 hour. T. parthenium at 50mg/ml concentration killed cysts (83%) and trophozite (100%) after 1 hour, respectively. T. parthenium chloroformic extract had a better effect on cyst and trophozoite of Giardia at 50 mg/ml after 1 hour exposure than A. annua extract. Conclusion: According to this study, A. annua and T. parthenium chlorofomic extracts could be considered as a more effective anti-giardial agent. Chloroformic extract of T. parthenium was also shown the anti-giardial activity compared with A. annua and control groups at all exposure times. Therefore, in the future research using these plants are recommended against Giardia in low concentration in the in vivo, also to find fractions of the pharmacological effects of these plants. .

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“…Its leaves have been used in folk medicine for the treatment of diarrhea (Brandelli et al, 2009), inflammation (Schapoval et al, 1994), hyperglycemia, hyperlipidemia, hypertriglycemia (Arai et al, 1999;Matsumura et al, 2000) and hypertension (Consolini et al, 1999). In addition, they have been used as an antimalarial and spasmolytic agent (Wazlawik et al, 1997;Morioka et al, 2000) as well as an inhibitor of DNA polymerase, maltase, sucrase, and α-glucosidase (Lee et al, 2000;Matsumura et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Influence of foliar nutrients on phenol levels in leaves of Eugenia uniflora

Santos¹,

Fortes²,

Ferri³

et al. 2011

Rev. bras. farmacogn.

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Eugenia unifl ora L., Myrtaceae, leaves contain high amounts of phenolic compounds which are responsible for several pharmacological activities. In order to evaluate the phenolics seasonal variation leaves were analysed on a monthly basis during the period of two years for the contents of hydrolysable tannins, total phenols, fl avonoids, and nutrients (N, P, K, S, Ca, Mg, Mn, Zn, Cu, and Fe). Results were correlated with climate conditions (rainfall, humidity, and mean temperature) by Principal Component and Cluster Analysis which allowed four groups to be distinguished with respect to the age of the leaves and the content of some metals. Young leaves were characterised by high levels of Zn and nitrogen whereas old leaves contained high levels of Fe and calcium, and both groups had moderate amounts of phenolics. Adult leaves were divided in two groups and results revealed that while one group had the highest levels of all phenols and lowest amounts of Mn and Cu, the other showed opposite quantities. The Canonical Correlation Analysis confi rmed a highly signifi cant negative correlation between phenol contents and Mn and Cu. These facts suggested that fl avonoids and tannins production depends of the amounts of foliar nutrients, Cu and Mn in particular, which are cofactors of enzymes involved in phenol degradation and lignin biosynthesis. This knowledge can improve this specie cultivation in order to enhance the phenolic compounds concentration.

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Indigenous traditional medicine: in vitro anti-giardial activity of plants used in the treatment of diarrhea

Cited by 48 publications

References 30 publications

Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine

Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine

Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro

Influence of foliar nutrients on phenol levels in leaves of Eugenia uniflora

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