Indirect Carcinoma-stromal Interaction Enhances Invasion of Both Fibroblast and Tumor Cells on a Microfluidic Co-culture Device

Abstract

Fibroblasts get significant involvement in cancer development and progression. In this work, a microfluidic non-contact co-culture platform has been presented to investigate interactions between tumor cells and normal fibroblasts. Human lung fibroblast (HLF) and lung carcinoma cell line (A549) were introduced in neighbouring, separated, regions under well-controlled laminar flows. Communication could be established through soluble factors. Invasion capabilities of both HLF and A549 were mutually enhanced remarkably. Moreover, HLF activation was recognized by more expression of α-smooth muscle actin (α-SMA), implying tumor cells might facilitate the transdiffernentiation of normal fibroblasts in a paracrine fashion to obtain a more supportive microenvironment. This in situ analysis of cells communication experiencing paracrine pathway gives us new insight into cellular events and anti-tumor therapy.

L-theanine mechanism and application in the treatment of breast cancer

“…The tumor microenvironment also plays an important role in the development of breast cancer [9]. Tumor-associated fibroblasts, immune cells, and other stromal cells can secrete growth factors and cytokines that promote tumor growth and invasion [10]. Similarly, angiogenesis, the formation of new blood vessels to provide nutrients and oxygen to the tumor, is also an important factor in tumor development.…”