Indirect chemiluminescence detection of amino acids separated by capillary electrophoresis

Batch-Type Detection Cell Using a Peroxyoxalate Chemiluminescence System for Capillary Electrophoresis

“…The following items are considered to be the cause: 1, injected sample; 2, buffer solution migrated into the cell; 3, decomposition due to electrolytic oxidation at an anionic electrode. 20 Repeated injection tests were carried out with 5 and 10 min run times for one injection. The relative CL intesity of Dns-Trp was plotted as a function of the injection number (Fig.…”

On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary

“…[1][2][3][4] The most commonly used detection mode in CE is UV absorption. More sensitive detection in CE can be achieved with laser-induced fluorescence, 5,6 electrochemical, 7,8 and chemiluminescence (CL) [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] approaches.…”

“…One is the batch-type [12][13][14][15][16][17]26,27 and the other is the flow-type. [18][19][20][21][22][23][24][25] The batch-type simply immerses the outlet end of a separation capillary into an outlet reservoir containing certain CL reagents and electrolyte buffer. The batch-type provides high sensitivity but is problematic in reproducibility and separation efficiency.…”

“…29 The coupling of CE-CL offers significantly high sensitivity and selectivity. [30][31][32] To date, a number of CL systems involving luminol, [10][11][12][13][14][15][16][17][18][19][20][21]28 peroxyoxalate, 9,10 permanganate, 24,27 ruthenium(II) complex, 25,26 acridinium esters 23 and firefly luciferase 15 have been utilized for the measurement of dyestuffs, proteins, amino acids, peptides, liposomes, alkaloids, phenols, ATP and metal ions with CE. Two main types of CL detection have been designed for CE.…”

Flow Injection Chemiluminescence Determination of Amino Acids by Oxidation with N-Bromosuccinimide

“…3 Bobbitt and Nieman developed electrochemiluminescence methods for the determination of amino acids 4,5 based on Ru(bpy)3 3+ . Some studies involved enzymes, [6][7][8] which made the experiment more expensive and required more care for maintaining the activity of the enzyme; Other CL methods for their determinations were all based on indirect CL reactions by amino acids, or by using a CL reagent as a label, [9][10][11][12][13] which would cause much complexity in the operation process, and would reduce the detection sensitivity.…”