Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance

Estévez, M.‐Carmen; Belenguer, J.; Gomez-Montes, Silvia; Miralles, Javier; Escuela, Alfonso Medina; Montoya, Ángel; Lechuga, Laura M.

doi:10.1039/c2an36094b

Cited by 42 publications

(25 citation statements)

References 31 publications

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“…Plasmonics is leading to significant advancements and innovations in photonic technologies including biomedicine, energy harvesting and telecommunications . For biomedical applications, plasmon‐based bio nanosensors consisting of metallic structures could overcome limitations of conventional biosensors through their unique ability to manipulate light at nanoscale dimensions.…”

mentioning

confidence: 99%

Plasmonically Enhanced Vibrational Biospectroscopy Using Low‐Cost Infrared Antenna Arrays by Nanostencil Lithography

Aksu

Çetin

Adato

et al. 2013

Advanced Optical Materials

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798 wileyonlinelibrary.com COMMUNICATION www.MaterialsViews.com www.advopticalmat.dePlasmonics [1][2][3][4][5][6][7][8] is leading to signifi cant advancements and innovations in photonic technologies including biomedicine, [ 9,10 ] energy harvesting [ 11,12 ] and telecommunications. [ 13 ] For biomedical applications, plasmon-based bio nanosensors [14][15][16][17] consisting of metallic structures could overcome limitations of conventional biosensors through their unique ability to manipulate light at nanoscale dimensions. Plasmonic biosensors could enable detection of medically important proteins and high throughput screening of their interactions. [ 18 ] One of the powerful and label free optical techniques for determining structural and conformational properties of proteins and protein interactions is Infrared (IR) Spectroscopy. [ 19 ] However, sensitivity of conventional IR spectroscopy is limited. Thus, relatively large quantities of analytes are required to have suffi cient absorption signal. Recently, resonant plasmonic nanoantennas have been used to enhance the absorption signals of surface bound protein monolayers. [20][21][22][23][24][25] The general concept is to electromagnetically couple the vibrational modes of the proteins to the optical modes of the nanoantennas. Plasmonic antennas boost the interaction between the light and the biomolecules through strongly enhanced near fi elds and increase the sensitivity to attomolar levels. [ 26 ] Such near fi eld interactions can be controlled through the geometry and composition of the metallic nanostructures in arrays. [ 27,28 ] Therefore even slight tailoring of the nanoantenna and its arrangement could signifi cantly alter the performance of surface enhanced IR spectroscopy technique. In order to realize precisely tailored plasmonic antennas, highly controllable nanofabrication techniques offering fl exible design capability are needed. Conventional electron and ion beam based lithography tools enable tremendous nanoscale feature fl exibility. However their limitations such as high operational cost and low throughput have motivated development of new nanofabrication techniques. There are a few inexpensive innovative approaches demonstrated recently including nano imprinting, [ 29 ] PEEL (a combination of phase-shifting photolithography, etching, electron-beam deposition and lift-off of the fi lm), [ 30 ] hole mask colloidal lithography, [ 31 ] and nano bridging. [ 32 ] However they either require multiple pattern transfer steps that limits the resolution or they lack the ability of precise arraying and shape control at nanoscale dimensions.Towards this end, we recently presented that nanostencil lithography (NSL) could be utilized for high resolution, high throughput, and low cost nanofabrication of plasmonic nanoantennas with great design fl exibility on conventional as well as fl exible and polymeric substrates. [ 33,34 ] Uniquely, NSL preserves the fl exibility of beam based lithography tools for creating nanoantennas in a variety of shapes and arran...

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confidence: 99%

Plasmonically Enhanced Vibrational Biospectroscopy Using Low‐Cost Infrared Antenna Arrays by Nanostencil Lithography

Aksu

Çetin

Adato

et al. 2013

Advanced Optical Materials

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“…During last years, we have demonstrated the suitability of our home-made (nano)plasmonic devices for clinical and environmental diagnostics, as for: (i) detection of toxic environmental pollutants in water and food [4,5], (ii) determination of highly structured RNA strands [6], (iii) identification of human growth hormone isoforms for doping control [7]; (iv) the recognition of Escherichia coli and Salmonella typhimurium flagellin to prevent intestinal infections [8], or (v) the study of the angiogenic response of carcinoma living cells during tumor growth and proliferation [9], among others. In all cases, plasmonic sensing method has shown excellent robustness with high reproducibility, rendering in a valuable tool for fast diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Nanoplasmonic Biosensors for Label-free Deciphering of Cellular Pathways

Huertas

Lechuga

2014

Latin America Optics and Photonics Conference

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“…The most notable attraction in SPR-based immunosensors is the highly specific detection of small molecules with extraordinarily low detection limits for a wide variety of analytes in complex matrices [7,[13][14][15][16][17][18] Most of the reported SPR developments correspond to competitive inhibition immunoassays. A usual strategy comprises the immobilization of a protein-analyte derivative (the carrier protein is typically serum albumin) and the evaluation of the binding of anti-analyte antibodies in a mixed solution containing the analyte in unknown concentration.…”

Section: Introductionmentioning

confidence: 99%

SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System

Millone

Ramírez

Chain

et al. 2016

Journal of Nanomaterials

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Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins. These substances typically have low molecular weight, making it necessary to perform competitive inhibition immunoassays. In most of the cases, the strategy is to immobilize a protein derivative of the analyte, which usually involves the appearance of nonspecific protein binding which limits the detection range of the assay. In this work we present results of a poly-L-lysine (Au-MUA-PLL) based sensor platform for quantitative determination of 2,4-dinitrophenol as model system for small molecular weight substances detection. The prepared sensor chip was characterized by means of Atomic Force Microscopy, Surface Plasmon Resonance, and Surface Enhanced Raman Spectroscopy. Experiments verified the absence of nonspecific protein adsorption to Au-MUA-PLL surfaces and the improvement of the competitive inhibition assays performance in comparison with single and mixed thiol self-assembled monolayers. The possibility of directly immobilizing 2,4-dinitrophenol to the poly-L-lysine containing platforms leads to an improvement in the detection of the soluble analyte by the competitive inhibition assay avoiding undesirable nonspecific protein adsorption. Therefore, Au-MUA-PLL surfaces constitute a suitable alternative for quantitative detection of small molecules when nonspecific adsorption cannot be avoided.

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Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance

Cited by 42 publications

References 31 publications

Plasmonically Enhanced Vibrational Biospectroscopy Using Low‐Cost Infrared Antenna Arrays by Nanostencil Lithography

Plasmonically Enhanced Vibrational Biospectroscopy Using Low‐Cost Infrared Antenna Arrays by Nanostencil Lithography

Nanoplasmonic Biosensors for Label-free Deciphering of Cellular Pathways

SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System

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