Indirect organogenesis and in vitro bulb formation of Pancratium maritimum

Yasemin, Sara; Köksal, Nezihe; Büyükalaca, Saadet

doi:10.1007/s11240-023-02545-z

Cited by 2 publications

(4 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lower sucrose concentrations led to sluggish growth, while concentrations exceeding 4% resulted in cell death, as evidenced by the blackening of the callus. Different sucrose, BA, and 2,4D concentrations in MS media affected the growing bulbs in in vitro conditions for P. maritimum [95].…”

Section: Stage 3: Bulb Growthmentioning

confidence: 99%

“…The induction of in vitro bulblets is primarily influenced by the temperatures and storage durations of the mother plant. Yasemin et al [95] emphasized the significance of explant types regarding factors such as callus color, callus hardness, callus fragility, callus formation rate, embryogenic callus rate, and callus growth rate of Pancratium maritimum.…”

Section: Explant Choicementioning

confidence: 99%

“…Seeds of P. maritimum were used as explants in some studies, and surface sterilization was successful [95,124,126,130]. However, surface sterilization of the flower bulbs is not quite easy.…”

Section: Stage 1: Establishment Of Aseptic Culturementioning

confidence: 99%

“…However, in the literature, we have several successful examples. Yasemin et al [95] transferred the P. maritimum plantlets into sand, sand:peat (1:1), and peat, and the survival rates were more than 90%. Rafiq et al [131] transferred the rooted plantlets into media containing perlite and vermiculite (1:1).…”

Section: Stage 5: Ex Vitro Acclimatization and Growthmentioning

confidence: 99%

See 3 more Smart Citations

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

Yasemin,

Beruto

2024

Horticulturae

Self Cite

View full text Add to dashboard Cite

This comprehensive review scrutinizes tissue culture and micropropagation methodologies in geophytes, focusing on bulbous plants. The examination encompasses key stages, including somatic embryogenesis, bulb growth, dormancy breaking, and planting. Studies underscore the pivotal role of plant growth regulators (PGRs) in plant regeneration and bulb growth. Bioreactor systems for healthy plant regeneration, rooting methods, acclimatization strategies, and considerations for ex vitro survival are elucidated. The review also delves into somaclonal variation dynamics and acknowledges the burgeoning field of gene editing, particularly Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) studies, as a promising avenue for enhancing valuable compound content in geophytes. In addition to addressing challenges in flower bulb micropropagation, this review briefly highlights emerging opportunities, including the potential integration of artificial intelligence (AI) to optimize culture conditions, predict growth parameters, and enhance efficiency in bulb production. The conclusion emphasizes the necessity of a multifaceted approach integrating biochemistry, physiology, and molecular biology to address existing challenges and improve tissue culture protocols for diverse geophyte species. This review article also intends to highlight how tissue culture techniques could contribute to the development and valorization of flower bulbs in today’s scenario of the ornamental industry.

show abstract

Section: Stage 3: Bulb Growthmentioning

confidence: 99%

Section: Explant Choicementioning

confidence: 99%

Section: Stage 1: Establishment Of Aseptic Culturementioning

confidence: 99%

Section: Stage 5: Ex Vitro Acclimatization and Growthmentioning

confidence: 99%

See 2 more Smart Citations

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

Yasemin,

Beruto

2024

Horticulturae

Self Cite

View full text Add to dashboard Cite

show abstract

Integrated GC-MS and UPLC-ESI-QTOF-MS based untargeted metabolomics analysis of in vitro raised tissues of Digitalis purpurea L

Bansal,

Mujib,

Mamgain

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Digitalis purpurea L. is one of the important plant species of Nilgiris, Kashmir and Darjeeling regions of India, belonging to the family Plantaginaceae, with well-known pharmacological applications. In the present investigation, an in vitro culture technique of indirect shoot organogenesis of D. purpurea is being explored; the biochemical attributes, the antioxidant activities and the metabolomic analyses were made by utilizing untargeted Gas Chromatography-Mass Spectrometry (GC-MS) and Ultra Performance Liquid Chromatography coupled with electronspray ionization/quadrupole-time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS) approaches. Initially, the leaf explants were used for callus induction and proliferation and maximum callusing frequency (94.44%) and fresh biomass (4.9 g) were obtained on MS, fortified with 8.8 µM BAP (6-benzyl amino purine) + 0.9 µM 2,4-D (2,4-dichlorophenoxyacetic acid), subsequently shoot formation (indirect organogenesis) was noted on the same MS medium with a shoot induction frequency of 83.33%. Later on, the biochemical and antioxidant potential of in vivo-, in vitro grown leaf and leaf derived callus were assessed. Significantly higher total phenol, flavonoid, DPPH (2,2-diphenyl-1-picrylhydrazyl), POD (peroxidase) and SOD (superoxide dismutase) activities were noticed in in vitro grown callus and leaf tissues compared with field grown leaf. The GC-MS analysis of each methanolic extract (in vivo-, in vitro derived leaf and leaf derived callus) displayed the presence of more than 75 bioactive compounds viz loliolide, stigmasterin, alpha-tocopherol, squalene, palmitic acid, linoleic acid, beta-amyrin, campesterol etc. possessing immense therapeutic importance. The UPLC-MS based metabolite fingerprinting of each methanolic extracts were conducted in both positive and negative ionization mode. The obtained results revealed variation in phytochemical composition in field - and laboratory grown tissues, indicating the impact of in vitro culture conditions on plant tissues. The detected phytocompounds belongs to various classes such as flavonoids, steroids, terpenoids, carbohydrates, tannins, lignans etc. The medicinally important metabolites identified were 20, 22-dihydrodigoxigenin, digoxigenin monodigitoxoside, apigenin, luteolin, kaempferide, rosmarinic acid, nepitrin and others. The results of the present study suggest that in vitro culture of D. purpurea could successfully be utilized for the novel drug discovery by producing such important phytocompounds of commercial interest in shorter duration without harming the plants’ natural population.

show abstract

Indirect organogenesis and in vitro bulb formation of Pancratium maritimum

Cited by 2 publications

References 36 publications

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

Integrated GC-MS and UPLC-ESI-QTOF-MS based untargeted metabolomics analysis of in vitro raised tissues of Digitalis purpurea L

Contact Info

Product

Resources

About