Indirect organogenesis and somatic embryogenesis for regeneration of Rauvolfia serpentina L. from root explants

Abstract

Highest frequencies (75%) of friable embryogenic nodular calli were recorded from the root explants in the presence of MS + 4.0 mg/l BAP and 4.0 mg/l NAA. In combinations of BAP and NAA 45.4% callus produced direct plantlets formation and 36.3% only from aerial part of shoots. MS medium with 1.0 mg/l BAP and 0.5 mg/l Kn produced compact callus where 13.3% produced direct plantlets formation and 45.7% produced only shoot formation. Well-developed globular somatic embryos were found to form when the callus were cultured more than 6 - 8 weeks on MS with 1.0 mg/l BAP and 0.5 mg/l Kn. After acclimatization the well rooted plantlets were transferred to pot and grown successfully in soil.

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

“…The results are in accordance with Hesami and Dhaneshvar [ 47 ], who found that 1.5 mg/L BAP along with 0.15 mg/L IBA showed 100% shoot regeneration frequency. Contrary to our observation, BAP with NAA was proved to be an efficient combination for indirect shoot regeneration in Digitalis ferruginea [ 37 ], Rauvolfia serpentina [ 48 ] and Aspilia africana [ 49 ].…”