Indirect organogenesis of Sechium edule (Jacq.) Swartz

Soto-Contreras, Anell; Núñez-Pastrana, Rosalía; Rodríguez-Deméneghi, Marco Vinicio; Aguilar‐Rivera, Noé; Galindo-Tovar, María Elena; Ramírez-Mosqueda, Marco A.

doi:10.1007/s11627-022-10304-6

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…The concentration did not show differences in callus fresh weight, diameter, height, volume, or root formation. Similar results were obtained by Soto-Contreras et al [39], who evaluated the effect of 0.5, 1.0, and 1.5 mg L −1 concentrations of 2,4-D on the induction of callus formation from different vitroplant explants of S. edule. Their results show that with the highest concentration, the nodal segments recorded 100% formation of white calli and a fresh weight of 1.74 g, while the calli of the leaf segments were greenish and had a fresh weight of 0.35 g.…”

Section: Experimental Phase 2: Callus Formation Protocolsupporting

confidence: 87%

“…A significant number of research works have focused on the in vitro establishment, conservation, and regeneration of S. edule [22,[32][33][34][35][36] in addition to clonal propagation, rooting, and acclimatization protocols [22,37,38]. However, unlike S. edule, other species of the genus, such as S. compositum, have not been studied to define explant-based callus formation protocols [39]. Callus formation is the basis of massive in vitro propagation through indirect organogenesis or indirect somatic embryogenesis [40,41]; however, no such protocol has been developed for wild species.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

de la Luz,

Román,

Jorge

et al. 2024

Horticulturae

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the shoot propagation, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL−1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds and shoots and the shoot height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L−1 of BA and 0.1 mg L−1 of TDZ, as well as the MS base culture medium. The validation of the results of the first phase (0.1 mg L−1 of BA) was compared with the MS in an independent experiment against the control (n = 50 repetitions), obtaining a height of 52 mm, 1.36 shoots, and 9.22 buds, suggesting that this concentration is adequate for the purpose, surpassing the MS control (MS culture medium alone). Of the total volume of roots obtained with packed bud structure in the previous experimental sample, it was reduced to 14% (n = 50). The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L−1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. Callus induction in S. compositum was better when using the stem in a medium with 2.0 mg L−1 of 2,4-D with a value of 97.8% around the explant. The addition of 500 mg L−1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callus formation of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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Section: Experimental Phase 2: Callus Formation Protocolsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

de la Luz,

Román,

Jorge

et al. 2024

Horticulturae

View full text Add to dashboard Cite

show abstract

“…The concentration did not show differences in callus fresh weight, diameter, height, volume, and root formation. Similar results were obtained by Soto-Contreras et al [39] who evaluated the effect of 0.5, 1.0, and 1.5 mg L -1 concentrations at of 2,4-Dichlorophenoxyacetic acid on the induction of callus formation from different vitroplant explants of S. edule. Their results show that, with the highest concentration, the nodal segments recorded 100% formation of white calli and a fresh weight of 1.74 g, while the calli of the leaf segments were greenish and had a fresh weight of 0.35 g.…”

Section: Experimental Phase 2: Callogenesis Inductionsupporting

confidence: 86%

“…A significant number of research works have focused on the in vitro establishment, conservation, and regeneration of S. edule [22,[32][33][34][35][36] in addition to clonal propagation, rooting, and acclimatization protocols [22,37,38]. However, unlike the case of other species, explant-based callogenesis protocols have not been studied [39]. Callogenesis is the basis for massive in vitro propagation through indirect organogenesis or indirect somatic embryogenesis [40,41]; however, no such protocol has been developed for wild species.…”

Section: Introductionmentioning

confidence: 99%

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Luz,

Román,

Mar

et al. 2023

Preprint

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the multiplication of seedlings, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL-1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds, shoots, and seedling height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L-1 of BA and 0.1 mg L-1 of TDZ, as well as the MS base culture medium. With 0.1 mg L-1 of BA, 52-mm height, 1.36 shoots, and 9.22 buds were obtained, surpassing the MS control (MS culture medium alone); likewise, twice more roots (80%) and a 14% reduction in the bud structure were packed in 50 repetitions. The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L-1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. The results demonstrated that the induction of callogenesis in S. compositum has a better start from the stem in a medium with 2.0 mg L-1 of 2,4-D, whose value was 4.89 —equivalent to 97.8% callus formation around the explant. The addition of 500 mg L-1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callogenesis of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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