1993
DOI: 10.1006/excr.1993.1028
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Indispensable Role of Tissue-Type Plasminogen Activator in Growth Factor-Dependent Tube Formation of Human Microvascular Endothelial Cells in Vitro

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Cited by 93 publications
(51 citation statements)
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“…Human microvascular endothelial cells cultured on the surface of three-dimensional type I collagen gel have a cobblestone-like appearance in the absence of any angiogenic growth factor, however, they form tube-like structures when treated with EGF or TGF-␣ (44,47,53).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Human microvascular endothelial cells cultured on the surface of three-dimensional type I collagen gel have a cobblestone-like appearance in the absence of any angiogenic growth factor, however, they form tube-like structures when treated with EGF or TGF-␣ (44,47,53).…”
Section: Resultsmentioning
confidence: 99%
“…In these models with bovine aortic endothelial cells, cell migration or tubular morphogenesis is potentially stimulated by bFGF (1,37,39,51) or VEGF (5,30). In contrast, cell migration or tubular morphogenesis in human microvascular endothelial cells is highly responsive to TGF-␣/EGF (34,44,47,53) and to VEGF and IL-8 (62). IL-8 is another angiogenic factor that is produced at high levels in monocytes, macrophages (33), and human brain tumors (22,38,60,62).…”
mentioning
confidence: 99%
“…This study did not provide evidence for basal membrane remodeling because treatment of cultures with antibodies directed against either uPA or tPA induced only a 15% (not significant) decrease in the formation of capillary tubes. In this respect, our system differs from that of other human microvascular endothelial cells where tPA activity has been shown to be required for growth factor-induced tube formation (Sato et al, 1993). In our system, whether proteolytic activity is not absolutely required for tube formation, or whether matrix metalloproteases contribute primarily to this activity has not been determined.…”
Section: Role Of Hematopoietic Cytokinesmentioning
confidence: 95%
“…We have reported that EGF/TGFα-induced angiogenesis might be attributable to its signaling through direct interaction with vascular endothelial cells, 14,18) and also to angiogenesis-related factors that are produced from cancer cells and vascular endothelial cells, based on studies using various angiogenesis models. 14,19,20) However, it remains unclear whether EGF-induced angiogenesis in vivo is mediated by direct interaction with endothelial cells. The vascular endothelial cells in a number of tumor types are known to express EGFR, [21][22][23] and suppression of VEGF or EGF expression inhibited the growth of a wide variety of tumor types in a murine tumor model, while angiogenesis inhibitors that target VEGF receptor or EGFR reduced tumor growth, metastasis, and the microvessel density of human pancreatic cancer in nude mice.…”
Section: Direct Inhibition Of Egf Receptor Activation In Vascular Endmentioning
confidence: 99%