Indium

Geckler, Robert P.; Marchi, Louis E.

doi:10.1021/ed021p407

Cited by 2 publications

(3 citation statements)

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“…Functionally, this impairment has been said to reduce both the number of spleen cells expressing surface h l molecules and the serum level of hl [7, 8, 181. The few h l chains which are produced in SJL mice appear to be normal [7].…”

Section: Discussionmentioning

confidence: 96%

“…The murine h light chain system is particularly interesting since it utilizes only a very limited number of V and J genes (reviewed in [20]) and, therefore, it represents a useful tool for studying the B cell repertoire and the role of somatic mutations in the generation of diversity. Another peculiarity of this system is that some inbred strains, most notably the SJL, have a level of h l chain expression which, compared with most other mouse strains, is severely impaired (the rill0 phenotype) [7]. This defect is linked to the structural hl gene [8].…”

Section: Discussionmentioning

confidence: 99%

“…The hl light chain accounts for the majority (80%) of h Ig chains in BALB/c mice [ l , 61. Several inbred strains of mice, including the SJL strain, have been found to have a greatly reduced h l expression ( r h p phenotype) compared with other strains [7]. Genetic analyses have shown that the expression of the h l Ig chain can be attributed to a locus rhl which is either identical or closely linked to the structural gene [8].…”

Section: Introductionmentioning

confidence: 99%

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The regulatory locus rλ1 affects the level of λl light chain synthesis in lipopolysaccharide‐activated lymphocytes but not the frequency of λl ‐positive B cell precursors

Sanchez¹,

Primi²,

Levi-Strauss³

et al. 1985

Eur J Immunol

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Several strains of mice, most notably the SJL strain, have a greatly reduced level of circulating lambda 1 immunoglobulins (r lambda 1 lo phenotype) compared with other mice. The locus responsible for this phenotype has been shown to be closely linked to the structural C lambda 1 gene. Functionally this locus has been said to reduce the number of lymphocytes expressing surface lambda 1 molecules. In order to gain a better understanding of this phenomenon we compared the functional properties of activated B cells secreting lambda 1 immunoglobulins in the splenocytes of both BALB/c and SJL mice. Our results indicate that regulatory T cells, as well as regulatory ontogenetic processes, are not responsible for the r lambda 1 lo phenotype. In addition, limiting dilution analyses revealed that the number of lipopolysaccharide-sensitive precursors of lambda 1-secreting B cells was similar in the splenocytes of the two strains of mice tested. The quantity of lambda 1 molecules produced by a B cell clone, however, was found to be lower in SJL than in BALB/c mice. As the level of lambda 1 mRNA is greatly reduced in lipopolysaccharide blasts of SJL mice, as compared to the mRNA detected in BALB/c blasts, we conclude that the impairment responsible for the r lambda 1lo phenotype is probably transcriptional. We tentatively propose that sequences 5' to the C lambda 1 region are defective in their capacity to enhance the lambda 1 transcripts in SJL mice.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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