Individual aerosol exposure unit for small rodents using photometry

Gebhard, J.; Scheuch, G.; Gehr, Peter; Geiser, Marianne; Heyder, J.; Hof, Vinzenz Im

doi:10.1016/0021-8502(88)90112-7

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…At present, the fate of the remaining 94O, of particles is not known precisely. (i) Current experiments in our laboratory show that a large proportion of microbeads is trapped in the plastic cannula used, even when the dead space is kept to a minimum, as recommended by Gebhart et al (1988). Humidification due to the exhalation might be the cause of this.…”

Section: Biological Aspectsmentioning

confidence: 92%

Assessment of particle retention and clearance in the intrapulmonary conducting airways of hamster lungs with the fractionator¹

Geiser

Cruz‐Orive

Hof

et al. 1990

Journal of Microscopy

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SUMMARY A modified version of the fractionator was used to estimate the total number of polystyrene microspheres retained in the airways of hamster lungs at two different time points after inhalation. A systematic three‐stage subsampling procedure with known sampling fractions was adopted. First, each lung was cut into slices, from which primary disectors were sampled systematically with a known sampling fraction. From each primary disector, smaller sub‐disectors were subsampled, and the corresponding sampling fraction was estimated by point counting. Finally, a few particles were counted at the microscopic level in the sub‐disectors, and the final estimate of total particle number (which is unbiased irrespective of any tissue deformations) was easily computed as a product of the counted number times the reciprocal of the successive sampling fractions. The error variance of each estimate was assessed from the data using a new estimator. An average of 6% of the deposited particles were retained on the epithelial surface of the intrapulmonary conducting airways shortly after the inhalation, from which at least one‐third was already phagocytosed by macrophages. After 24 h, an average of 87% of the particles retained shortly after the inhalation had been cleared. The proportion of particles ingested by macrophages had increased to at least 87%, in three out of four animals studied.

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Section: Biological Aspectsmentioning

confidence: 92%

Assessment of particle retention and clearance in the intrapulmonary conducting airways of hamster lungs with the fractionator¹

Geiser

Cruz‐Orive

Hof

et al. 1990

Journal of Microscopy

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show abstract

Histological and Stereological Analysis of Particle Retention in the Conducting Airways of Hamster Lungs

Geiser¹,

Hof²,

Gehr³

et al. 1990

Journal of Aerosol Medicine

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Individual aerosol exposure unit for small rodents using photometry

Cited by 2 publications

References 3 publications

Assessment of particle retention and clearance in the intrapulmonary conducting airways of hamster lungs with the fractionator¹

Assessment of particle retention and clearance in the intrapulmonary conducting airways of hamster lungs with the fractionator¹

Histological and Stereological Analysis of Particle Retention in the Conducting Airways of Hamster Lungs

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Individual aerosol exposure unit for small rodents using photometry

Cited by 2 publications

References 3 publications

Assessment of particle retention and clearance in the intrapulmonary conducting airways of hamster lungs with the fractionator1

Assessment of particle retention and clearance in the intrapulmonary conducting airways of hamster lungs with the fractionator1

Histological and Stereological Analysis of Particle Retention in the Conducting Airways of Hamster Lungs

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Assessment of particle retention and clearance in the intrapulmonary conducting airways of hamster lungs with the fractionator¹

Assessment of particle retention and clearance in the intrapulmonary conducting airways of hamster lungs with the fractionator¹