Individual and combined effects of ammonia-N and sulfide on the immune function and intestinal microbiota of Pacific white shrimp Litopenaeus vannamei

Jiang, Li; Feng, Jianxiang; Ying, Rui; Yin, Fangmin; Pei, Surui; Lü, Jianguo; Guo, Jianlin; Li, Zufu

doi:10.1016/j.fsi.2019.06.020

Cited by 38 publications

(11 citation statements)

References 55 publications

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“…Ammonia stress is one of the biggest challenges facing shrimp aquaculture. Ammonia not only has a direct lethal effect on shrimp [7], but also inhibits the shrimp immune system and increases sensitivity to pathogens [60]. Breeding new varieties of ammonia-tolerant shrimp is therefore an important target of the shrimp aquaculture industry.…”

Section: Discussionmentioning

confidence: 99%

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng

Yang

et al. 2020

BMC Genomics

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Background Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. Results In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 17,338 polymorphic markers spanning 44 linkage groups, with a total distance of 6360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41–8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance. Conclusions In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.

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Section: Discussionmentioning

confidence: 99%

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng

Yang

et al. 2020

BMC Genomics

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“…An increase in SOD production was previously reported in M. japonicus and P. vannamei exposed to sulphide stress (Hsu & Chen, 2007). Though, SOD production is expected to increase, a study conducted by Jiang et al (2019) reported a increase in SOD at the beginning of the experiment reduction in SOD production when prolonged sulphide and ammonia stress is given. In the case of PO activity, increase was observed in sulphide exposed treatments under pH and hypoxic stress when compared to normoxic treatments.…”

Section: Discussionmentioning

confidence: 98%

Effect of sulphide in Pacific white shrimpPenaeus vannameiunder varying oxygen and pH levels

Thulasi

Muralidhar

Saraswathy

2020

Aquaculture Research

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Sulphide is one of the known environmental stressors, which potentially binds to cytochrome C oxidase (COX), a key enzyme in the electron transport chain, thereby blocking oxygen transport and ATP production. To ascertain the toxic effects of sulphide on Pacific white shrimp, Penaeus vannamei, two distinct exposure experiments were carried out with varying concentration of sulphide (0–1 mg/L), dissolved oxygen (normoxia and hypoxia) and pH values (8.2, 6 and 5). The activity of enzymes viz COX, superoxide dismutase (SOD), phenoloxidase and lactate accumulation was investigated. Outer membrane integrity and COX monomer separation were also done with the isolated crude mitochondrial preparations. Results indicated a significant reduction (p ≤ .05) in COX enzyme activity in sulphide exposed treatments when compared to control. The reduction was more intense when pH levels were reduced under hypoxia condition. Lactate accumulation as a result of anaerobic metabolism was found to be higher in hypoxic treatments. No significant difference (p ≥ .05) was observed in superoxide dismutase activity between the treatments, whereas phenoloxidase activity significantly decreased at higher concentration sulphide. Separation of mitochondrial proteins resulted in the identification of ~205 kDa of COX monomer, and significant damage was found in outer membrane integrity under hypoxia and pH treatments. From this study, it is evident that at a given concentration, sulphide is toxic to P. vannamei, and in association with hypoxia and low pH, they further intensify sulphide toxicity. Our results indicated that sulphide toxicity should not be considered as a single factor, rather it should be a considered as combination of factors.

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“…Ammonia stress is one of the biggest challenges facing shrimp aquaculture. Ammonia not only has a direct lethal effect on shrimp [7], but also inhibits the shrimp immune system and increases sensitivity to pathogens [56]. Breeding new varieties of ammonia-tolerant shrimp is therefore an important target of the shrimp aquaculture industry.…”

Section: Discussionmentioning

confidence: 99%

QTL and transcriptomic analyses for ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng¹,

Yang²,

Li³

et al. 2019

Preprint

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Background: Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. Results: In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 807,505 polymorphic markers spanning 44 linkage groups, with a total distance of 6,360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41–8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance. Conclusions: In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.

show abstract

Individual and combined effects of ammonia-N and sulfide on the immune function and intestinal microbiota of Pacific white shrimp Litopenaeus vannamei

Cited by 38 publications

References 55 publications

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Effect of sulphide in Pacific white shrimpPenaeus vannameiunder varying oxygen and pH levels

QTL and transcriptomic analyses for ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

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