Individual Osmotic Fragility Distribution: A New Parameter for Determination of the Osmotic Properties of Human Red Blood Cells

Abstract

The aim of our experiments was to characterise and to validate the osmotic fragility test when applied to human blood samples with no significant alterations of osmotic fragility but with a differentiating shape of the haemolysis curve. All experiments were carried out on human erythrocytes taken from the Regional Centre of Blood Donation and Blood Therapy in Wrocław. The washed erythrocytes were exposed to near-infrared radiation (NIR) or ozonated, and the osmotic fragility test was applied. The osmotic fragility, calculated from the experimental haemolysis curve for the control and cells irradiated for 15 min, is the same within the empirical error. Calculation of the first derivative of the haemolysis curve allowed us to visualise the changes in osmotic fragility distribution after exposure to NIR. By contrast, significant changes both to the osmotic fragility value and the distribution of osmotic properties were observed after an erythrocytes ozonation procedure. Description of cell osmotic properties requires at least two parameters—the value of osmotic fragility and the slope of the haemolysis curve in the region where absorbance sharply increases due to cell haemolysis.

Integrated Microfluidic System for Gene Silencing and Cell Migration

“…In less-salted solution, membrane expanded and became loose due to the change of osmotic pressure. [12] The results of improved delivery efficiency in less salted solution supported our hypothesis. Though using low PBS concentration solution can improve delivery efficiency, it resulted in more deaths.…”

Effect of alcohol consumption on haemorheology and osmotic fragility of subjects in Nnewi, South-eastern, Nigeria

“…The osmotic fragility test is used to gauge the level of haemolysis in a collected sample of a patient's blood and this is compared to a control sample. 20,21 There was a significant increase in osmotic fragility (MCF) in alcohol consumers. This is in consonance with the findings that alcohol and smoking causes membrane deformity.…”

Phytocomponents from Anacardium occidentale, Psidium guajava, and Terminalia catappa altered membrane osmotic stability of sickle erythrocytes

“…When erythrocytes are suspended in hypotonic sodium chloride (NaCl) solution of diminishing osmolality, the uptake of water by the erythrocyte is both instantaneous and quantitative. In practical terms, the quantity of hemoglobin released into the hypotonic solutions following hemolysis is a measure of erythrocyte osmotic fragility index [25][26][27]. Accordingly, osmotic fragility index defines the capacity of the erythrocytes to withstand hemolysis when subjected to osmotic stress [27].…”

“…In practical terms, the quantity of hemoglobin released into the hypotonic solutions following hemolysis is a measure of erythrocyte osmotic fragility index [25][26][27]. Accordingly, osmotic fragility index defines the capacity of the erythrocytes to withstand hemolysis when subjected to osmotic stress [27]. Osmotic fragility test, in which the population of hemolyzed erythrocytes is measured as a function of variability in osmolality of erythrocyte suspension, is primarily diagnostic of hereditary disorders of erythrocyte membrane cytoskeletal structures such as hereditary spherocytosis and other vast groups of the hemolytic anemias [27][28][29][30][31][32].…”

“…Accordingly, osmotic fragility index defines the capacity of the erythrocytes to withstand hemolysis when subjected to osmotic stress [27]. Osmotic fragility test, in which the population of hemolyzed erythrocytes is measured as a function of variability in osmolality of erythrocyte suspension, is primarily diagnostic of hereditary disorders of erythrocyte membrane cytoskeletal structures such as hereditary spherocytosis and other vast groups of the hemolytic anemias [27][28][29][30][31][32]. The extrinsic and intrinsic factors that influence erythrocyte osmotic fragility index are summarized elsewhere [27,30].…”