Individual radiosensitivity measured with lymphocytes may be used to predict the risk of fibrosis after radiotherapy for breast cancer

Hoeller, Ulrike; Borgmann, Kerstin; Bonacker, Michael; Kuhlmey, Antje; Bajrovic, Amira; Jung, H.; Alberti, Winfried; Dikomey, Ekkehard

doi:10.1016/j.radonc.2003.10.001

Cited by 104 publications

(62 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, the utility of the assay for predicting RIF was not supported, and has been abandoned due to the invasiveness of the sampling, difficulty of the assay and the length time required to determine the response. Greater success was achieved in correlating damage in lymphocytes with RIF, specifically lethal aberrations after 6 Gy exposure in vitro (117). This study found more fibrosis in patients with higher levels of in vitro induced lethal aberrations.…”

Section: Predictive Biomarkers For Radiation Fibrosismentioning

confidence: 75%

Isoform-Specific Activation of Latent Transforming Growth Factor β (LTGF-β) by Reactive Oxygen Species

et al. 2006

View full text Add to dashboard Cite

Section: Predictive Biomarkers For Radiation Fibrosismentioning

confidence: 75%

Isoform-Specific Activation of Latent Transforming Growth Factor β (LTGF-β) by Reactive Oxygen Species

et al. 2006

View full text Add to dashboard Cite

“…Lee et al (27) reported that radiation-induced micronuclei in lymphocytes of prostate cancer patients can be used to predict gastrointestinal or genitourinary toxicity. Recently, in a retrospective study, the number of lethal chromosomal aberrations in in vitro irradiated peripheral blood lymphocytes correlated well with radiation-induced fibrosis in patients with breast cancer (28).…”

Section: Discussionmentioning

confidence: 99%

CD4 and CD8 T-Lymphocyte Apoptosis Can Predict Radiation-Induced Late Toxicity: A Prospective Study in 399 Patients

Ozşahin

Crompton

Gourgou

et al. 2005

Clinical Cancer Research

193

115

View full text Add to dashboard Cite

Purpose: Predicting late effects in patients treated with radiation therapy by assessing in vitro radiation-induced CD4 and CD8 T-lymphocyte apoptosis can be useful in individualizing treatment. Experimental Design: In a prospective study, 399 curatively irradiated patients were tested using a rapid assay where fresh blood samples were in vitro irradiated with 8 Gy X-rays. Lymphocytes were collected and prepared for flow cytometric analysis. Apoptosis was assessed by associated condensation of DNA. The incidences of late toxicities were compared for CD4 and CD8 T-lymphocyte apoptoses using receiver-operating characteristic curves and cumulative incidence. Results: No association was found between early toxicity and T-lymphocyte apoptosis. Grade 2 and 3 late toxicities were observed in 31% and 7% of patients, respectively. More radiationinducedT-lymphocyte apoptosis was significantly associated with less grade 2 and 3 late toxicity (Gray's test, P < 0.0001). CD8 (area under the curve = 0.83) was more sensitive and specific than CD4. No grade 3 late toxicity was observed for patients with CD4 and CD8 values greater than 15% and 24%, respectively. The 2-year cumulative incidence for grade 2 or 3 late toxicity was 70%, 32%, and 12% for patients with absolute change in CD8 T-lymphocyte apoptosis of V16, 16 to 24, and >24, respectively.Conclusions: Radiation-induced T-lymphocyte apoptosis can significantly predict differences in late toxicity between individuals. It could be used as a rapid screen for hypersensitive patients to radiotherapy. In future dose escalation studies, patients could be selected using the apoptosis assay.

show abstract

“…However, other studies show no correlation between g-H2AX foci and acute [110] or late tissue damage [111]. Scoring of chromosome aberrations in ex vivo-irradiated lymphocytes has also been correlated with acute and late normal tissue damage in radiotherapy patients [112,113]. Chua et al [114] also showed a correlation between dicentrics, acentrics and rings and g-H2AX and late normal tissue damage in ex vivo-irradiated lymphocytes from breast cancer radiotherapy patients.…”

Section: Radiosensitivitymentioning

confidence: 99%

Deoxyribonucleic acid damage-associated biomarkers of ionising radiation: current status and future relevance for radiology and radiotherapy

Manning

Rothkamm²

2013

BJR

View full text Add to dashboard Cite

Diagnostic and therapeutic radiation technology has developed dramatically in recent years, and its use has increased significantly, bringing clinical benefit. The use of diagnostic radiology has become widespread in modern society, particularly in paediatrics where the clinical benefit needs to be balanced with the risk of leukaemia and brain cancer increasing after exposure to low doses of radiation. With improving long-term survival rates of radiotherapy patients and the ever-increasing use of diagnostic and interventional radiology procedures, concern has risen over the long-term risks and side effects from such treatments. Biomarker development in radiology and radiotherapy has progressed significantly in recent years to investigate the effects of such use and optimise treatment. Recent biomarker development has focused on improving the limitations of established techniques by the use of automation, increasing sensitivity and developing novel biomarkers capable of quicker results. The effect of low-dose exposure (0-100 mGy) used in radiology, which is increasingly linked to cancer incidences, is being investigated, as some recent research challenges the linear-no-threshold model. Radiotherapy biomarkers are focused on identifying radiosensitive patients, determining the treatment-associated risk and allowing for a tailored and more successful treatment of cancer patients. For biomarkers in any of these areas to be successfully developed, stringent criteria must be applied in techniques and analysis of data to reduce variation among reports and allow data sets to be accurately compared. Newly developed biomarkers can then be used in combination with the established techniques to better understand and quantify the individual biological response to exposures associated with radiology tests and to personalise treatment plans for patients.

show abstract

Individual radiosensitivity measured with lymphocytes may be used to predict the risk of fibrosis after radiotherapy for breast cancer

Cited by 104 publications

References 40 publications

Isoform-Specific Activation of Latent Transforming Growth Factor β (LTGF-β) by Reactive Oxygen Species

Isoform-Specific Activation of Latent Transforming Growth Factor β (LTGF-β) by Reactive Oxygen Species

CD4 and CD8 T-Lymphocyte Apoptosis Can Predict Radiation-Induced Late Toxicity: A Prospective Study in 399 Patients

Deoxyribonucleic acid damage-associated biomarkers of ionising radiation: current status and future relevance for radiology and radiotherapy

Contact Info

Product

Resources

About