Individual Variation of the Genetic Response to Bisphenol A in Human Foreskin Fibroblast Cells Derived from Cryptorchidism and Hypospadias Patients

Qin, Xian; Sone, Hirohito; Kojima, Yoshiyuki; Mizuno, Kentaro; Ueoka, Katsuhiko; Muroya, Koji; Miyado, Mami; Hisada, Aya; Zaha, Hiroko; Fukuda, T.; Yoshinaga, Jun; Yonemoto, Junzo; Kohri, Kenjiro; Hayashi, Yutaro; Fukami, Maki; Ogata, Tsutomu

doi:10.1371/journal.pone.0052756

Cited by 12 publications

(4 citation statements)

References 32 publications

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“…We carried out sequential passages to evaluate the growth of wild type and recombinant cells. We also assessed the PD value, which represents the number of cell divisions, calculated using the following formula; PD = log 2 (a/b), where “a” is the number of cells counted at each passage and “b” is the number of cells seeded at the start of each passage [18,19]. To obtain the value of “a”, cells were seeded at a concentration of 5×10 4 cells/well (the value of “b”) in a 6-well plate.…”

Section: Methodsmentioning

confidence: 99%

Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon)

et al. 2019

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The Bonin flying fox ( Pteropus pselaphon ) is one of the most critically endangered species of animals. The number of this species is estimated to be around 150; being classified at the top rank in the list by International Union of Animal Conservation. Our group previously showed that expression of CDK4, CYCLIN D1, and telomerase reverse transcriptase (TERT) efficiently induce immortalization of human, bovine, swine, monkey, and buffalo-derived cells. In this manuscript, we successfully established the primary cells from Bonin flying fox. We introduced CDK4, CYCLIN D1, and TERT into the primary cells. The established cells showed efficient expression of introduced genes at the protein level. Furthermore, the established cells were free from senescence, indicating it reached to immortalization. Moreover, we showed that interspecies somatic cell nuclear transfer of Bonin flying fox derived cell into bovine embryo allowed the development of the embryo to 8 cell stages. Our established cell has the potential to contribute to species conservation.

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Section: Methodsmentioning

confidence: 99%

Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon)

et al. 2019

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“…[67][68][69][70][71][72][73][74][75] However, there have only been a few studies on the associations between chemical exposure and expression of target regulatory genes involved in cryptorchidism. 76,77 Because genes and polymorphisms are involved in the effects of EEDs, metabolism might influence the risk of cryptorchidism. Further studies are needed to assess the effects of environmental chemicals on the incidence of cryptorchidism.…”

Section: Discussionmentioning

confidence: 99%

Effects of the prenatal environment on cryptorchidism: A narrative review

Mitsui

2021

Int J of Urology

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Cryptorchidism, the absence of testes from the scrotum, is the most common genital disorder in boys and a risk factor for reduced fertility and testicular cancer. The mechanism responsible for cryptorchidism involves two discrete stages: a transabdominal and an inguinoscrotal phase. These phases of testicular descent are regulated by the prenatal sex hormone environment, including levels of testosterone, insulin-like factor 3, and calcitonin gene-related peptide. Environmental endocrine disruptors, which are unfavorable environmental factors, may also affect testicular descent through prenatal sex hormones. This review examined the effects of environmental factors, particularly environmental endocrine disruptors, such as phthalates, organochlorine pesticides, diethylstilbestrol, bisphenol A, dioxins/dioxin-like compounds, and perfluoroalkyl substances, and parental lifestyles on the risk of cryptorchidism. Although some studies have shown that environmental endocrine disruptors can affect testicular descent by changing the hormonal environment during the prenatal period, no significant association has been established between exposure to environmental endocrine disruptors and the incidence of cryptorchidism. Therefore, the role played by environmental endocrine disruptor exposure (if any) in the pathogenesis of cryptorchidism remains unknown. Further studies are needed to examine these issues.

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“…Various changes in skin development and marker allocation have been shown to occur as a consequence [2, 12]. Foreskin derived fibroblasts from children with hypospadias react differently to endocrine stimuli, possibly based on genetic variation in these subjects [13]. Furthermore, a subcutaneous muscle layer, termed the “dartos fascia” has been shown to be disorganized in patients with hypospadias [14].…”

Section: Discussionmentioning

confidence: 99%

Langerhans cells in hypospadias: an analysis of Langerin (CD207) and HLA-DR on epidermal sheets and full thickness skin sections

et al. 2019

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BackgroundHypospadias are among the most common genital malformations. Langerhans Cells (LCs) play a pivotal role in HIV and HPV infection. The migration of LC precursors to skin coincides with the embryonic period of hypospadias development and genetic alterations leading to the formation of hypospadias impact the development of ectodermally derived tissues. We hypothesized that this might be associated with a difference in frequency or morphology of epidermal and dermal LCs in hypospadias patients.MethodsA total of 43 patients from two centers were prospectively included into this study after parental consent and ethics approval. Epidermal and dermal sheets were prepared from skin samples of 26 patients with hypospadias, 13 patients without penile malformations and 4 patients with penile malformations other than hypospadias. Immunofluorescence staining of sheets was performed with anti-HLA-DR-FITC and anti-CD207/Langerin-A594 antibodies. Skin sections from 11 patients without penile malformation and 11 patients with hypospadias were stained for Langerin. Frequencies as well as morphology and distribution of epidermal and dermal LCs on sheets and sections were microscopically evaluated. Cell counts were compared by unpaired t-tests.ResultsThere was no difference in frequency of epidermal LCs, Neither on sheets (873 ± 61 vs. 940 ± 84LCs/mm2, p = 0.522) nor on sections (32 ± 3 vs. 30 ± 2LCs/mm2, p = 0.697). Likewise, the frequency of dermal LCs (5,9 ± 0,9 vs. 7.5 ± 1.3LCs/mm2, p = 0.329) was comparable between patients with hypospadias and without penile malformation. No differences became apparent in subgroup analyses, comparing distal to proximal hypospadias (p = 0.949), younger and older boys (p = 0.818) or considering topical dihydrotestosterone treatment prior to surgery (p = 0.08). The morphology of the LCs was not different comparing hypospadias patients with boys without penile malformations.ConclusionsLCs are present in similar frequencies and with a comparable morphology and distribution in patients with hypospadias as compared to children without penile malformations. This suggests that patients with hypospadias are not different from patients with normal penile development considering this particular compartment of their skin immunity.

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Individual Variation of the Genetic Response to Bisphenol A in Human Foreskin Fibroblast Cells Derived from Cryptorchidism and Hypospadias Patients

Cited by 12 publications

References 32 publications

Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon)

Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon)

Effects of the prenatal environment on cryptorchidism: A narrative review

Langerhans cells in hypospadias: an analysis of Langerin (CD207) and HLA-DR on epidermal sheets and full thickness skin sections

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