Individualization of the Cutoff Value for Serum Squamous-Cell Carcinoma Antigen Using a Sensitive Enzyme Immunoassay

Takeshima, Nobuhiro; Nakamura, Kaoru; Takeda, Osamu; Morioka, Hiroshi; Tamura, H.; Takasugi, Nobuyoshi; Katô, Hiroshi

doi:10.1159/000217651

Cited by 24 publications

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“…The expression level of SCCA protein in cell lysate of the transfected cells was confirmed by IMx system (DaiNabot, Tokyo) (Takeshima et al, 1990). …”

Section: Expression Of the Scca Cdna In The Transfected Cellsmentioning

confidence: 99%

Squamous cell carcinoma antigen suppresses radiation-induced cell death

et al. 2001

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Summary Previous study has demonstrated that squamous cell carcinoma antigen (SCCA) 1 attenuates apoptosis induced by TNFα, NK cell or anticancer drug. In this study, we have examined the effect of SCCA2, which is highly homologous to SCCA1, but has different target specificity, against radiation-induced apoptosis, together with that of SCCA1. We demonstrated that cell death induced by radiation treatment was remarkably suppressed not only in SCCA1 cDNA-transfected cells, but also in SCCA2 cDNA-transfected cells. In these transfectants, caspase 3 activity and the expression of activated caspase 9 after radiation treatment were suppressed. Furthermore, the expression level of phosphorylated p38 mitogen-activated protein kinase (p38 MAPK) was suppressed compared to that of the control cells. The expression level of upstream stimulator of p38 MAPK, phosphorylated MKK3/MKK6, was also suppressed in the radiation-treated cells. Thus, both SCCA1 and SCCA2 may contribute to survival of the squamous cells from radiation-induced apoptosis by regulating p38 MAPK pathway.

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“…The expression level of SCCA protein in cell lysate of the transfected cells was confirmed by IMx system (DaiNabot, Tokyo) (Takeshima et al, 1990). …”

Section: Expression Of the Scca Cdna In The Transfected Cellsmentioning

confidence: 99%

Squamous cell carcinoma antigen suppresses radiation-induced cell death

et al. 2001

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“…Western blotting was performed using the enhanced chemiluminescence (ECL) detection system (Amersham, Arlington Heights, IL, USA) with a monoclonal antibody (mAb-13) to SCC Ag-1 (Suminami et al, 1991). The expression level of the SCC Ag-1 protein was also analysed by a sensitive immunoassay method (IMx, Dainabot, Tokyo, Japan), as described previously (Takeshima et al, 1990).…”

Section: Expression Of the Scc Ag-1 Cdna In Transduced Cellsmentioning

confidence: 99%

Inhibition of apoptosis in human tumour cells by the tumour-associated serpin, SCC antigen-1

et al. 2000

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SummaryThe squamous cell carcinoma antigen (SCC Ag) is a tumour-associated protein and a member of the serine protease inhibitor (serpin) family. The SCC Ag has been used as a serologic tumour marker for SCC progression, and its elevated serum levels are a risk factor for disease relapse. However, the biologic significance of this intracytoplasmic protein in cancer cells remains unknown. In this report, we demonstrated that apoptosis induced by 7-ethyl-10-hydroxycamptothecin, tumour necrosis factor-α (TNF-α) or interleukin (IL)-2-activated natural killer (NK) cells was significantly inhibited in tumour cells transduced with the SCC Ag-1 cDNA, as compared to control cells in vitro. Also, inhibition of the SCC Ag-1 expression in tumour cells by transfection of antisense SCC Ag-1 cDNA was accompanied by significantly increased sensitivity of these cells to apoptosis induced by etoposide or TNF-α. The mechanism of protection of tumour cells from apoptosis involved inhibition of caspase-3 activity and/or upstream proteases. In vivo, tumour cells overexpressing the SCC Ag-1 formed significantly larger tumours in nude mice than the SCC Ag-1-negative controls. Thus, overexpression of the SCC Ag-1, a member of the serpin family, in human cancer cells contributed to their survival by mediating protection from drug-, cytokine-or effector cell-induced apoptosis.

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“…These cells were mildly sonicated in ice-cold PBS and centrifuged at 15,000 rpm for 10 min at 4°C. The supernatants were analyzed by the IMx system (DaiNabot, Tokyo, Japan) to detect the expression of SCCA [24] and saved for further study.…”

Section: Cell Culture and Gene Transfectionmentioning

confidence: 99%

Specific Detection and Quantitation of <i>SCC Antigen 1</i> and <i>SCC Antigen 2</i> mRNAs by Fluorescence-Based Asymmetric Semi-Nested Reverse Transcription PCR

Murakami¹,

Suminami²,

Sakaguchi³

et al. 2000

Tumor Biology

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Squamous cell carcinoma antigen (SCCA) is expressed in normal squamous epithelia and malignant squamous cell tissues. The serum level of SCCA has been used to evaluate treatment efficacy, clinical course of disease, and recurrence. SCCA is produced by at least two genes (SCCA1 and SCCA2); both of them have been located on chromosome 18q21.3. It has been difficult to examine the expression levels of SCCA1 and SCCA2 mRNAs separately because of their high homology at nucleotide level. In the present study, asymmetric semi-nested reverse transcription PCR, based on the principle of fluorescence energy transfer, enabled to quantitate the copy numbers of both SCCA1 and SCCA2 mRNAs. Using this method, the expression levels of these mRNAs were evaluated in normal and malignant squamous tissues. The copy number of SCCA2 mRNA was higher in malignant tissues than in normal tissues, while those of SCCA1 mRNA did not significantly differ between normal and malignant tissues. These data indicate that specific quantitation of the expression level of SCCA2 mRNA may be useful for the diagnosis and management of patients with squamous cell carcinoma.

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Individualization of the Cutoff Value for Serum Squamous-Cell Carcinoma Antigen Using a Sensitive Enzyme Immunoassay

Cited by 24 publications

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Squamous cell carcinoma antigen suppresses radiation-induced cell death

Squamous cell carcinoma antigen suppresses radiation-induced cell death

Inhibition of apoptosis in human tumour cells by the tumour-associated serpin, SCC antigen-1

Specific Detection and Quantitation of <i>SCC Antigen 1</i> and <i>SCC Antigen 2</i> mRNAs by Fluorescence-Based Asymmetric Semi-Nested Reverse Transcription PCR

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