Indocyanine Green-based Glow Nanoparticles Probe for Cancer Imaging

Abstract

Indocyanine green (ICG) is one of the FDA-approved near infra-red fluorescent (NIRF) probes for cancer imaging and image-guided surgery in the clinical setting. However, the limitations of ICG include poor photostability, high concentration toxicity, short circulation time, and poor cancer cell specificity. To overcome these hurdles, we engineered a nanoconstruct composed of poly (vinyl pyrrolidone) (PVP)-indocyanine green that is cloaked self-assembled with tannic acid (termed as indocyanine green-based glow nanoparticles probe, ICG-Glow NPs) for the cancer cell/tissue-specific targeting. The self-assembled ICG-Glow NPs were confirmed by spherical nanoparticles formation (DLS and TEM) and spectral analyses. The NIRF imaging characteristic of ICG-Glow NPs was established by superior fluorescence counts on filter paper and chicken tissue. The ICG-Glow NPs exhibited excellent hemo and cellular compatibility with human red blood cells, kidney normal, pancreatic normal, and other cancer cell lines. An enhanced cancer-specific NIRF binding and imaging capability of ICG-Glow NPs was confirmed using different human cancer cell lines and human tumor tissues. Additionally, tumor-specific binding/accumulation of ICG-Glow NPs was confirmed in MDA-MB-231 xenograft mouse model. Collectively, these findings suggest that ICG-Glow NPs have great potential as a novel and safe NIRF imaging probe for cancer cell/tumor imaging. This can lead to a quicker cancer diagnosis facilitating precise disease detection and management.

Near-Infrared Light-Responsive Multifunctional Photothermal/Photodynamic Titanium Diboride Nanocomposites for the Treatment of Antibiotic-Resistant Bacterial Infections

“…In addition, we investigated the in vitro cytotoxicity of free ICG (Figure S10). The results showed that the ICG concentration released by the composite was not cytotoxic, and the cell viability rate was higher than 95%, which is consistent with other previous studies . At the same time, hemolysis test (Figure b) showed that TiB 2 -QCS-ICG at different concentrations showed excellent blood compatibility after incubation with blood cells, and even at higher concentrations, the hemolysis rate was still significantly lower than the standard 5% specified in the Pharmacopoeia of the People’s Republic of China (*** p < 0.001).…”

Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing

“…As previously described, chicken eggs were purchased from a local organic chicken farm and incubated at 37.8 • C, a pCO2 of 5%, and humidity calibrated to 63% [32][33][34]. First, an approximately 5 mm × 5 mm hole was cut into the eggshell using sterile scissors, followed by a second larger hole (1 cm × 1 cm) in the longitudinal side of the eggshell.…”

“…Due to its ability to fluorescence when stimulated with light in the infrared wavelength range, ICG is known for its use in liver function analysis [29], lymph node imaging [30], and intraoperative tumor tissue visualization [31]. Based on its composition, it interacts with macromolecular serum proteins such as albumin, known to be enriched in most cancers [32]. In clinical use, chemotherapy is applied to patients intra-venously, as we also perform in our setting.…”

Fluorescence imaging-guided surgery: current status and future directions

“…With a short half-life of 150–180 seconds and exclusive hepatic clearance, ICG allows for continuous administration. 88…”