Indole-3-acetic Acid Synthesis in Tumorous and Nontumorous Species of <i>Nicotiana</i>

Liu, S T; Katz, Courtney; Knight, C.A.

doi:10.1104/pp.61.5.743

Cited by 16 publications

(7 citation statements)

References 12 publications

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“…[20] we did not detect any tryptamine, either in normal or in transformed tissue culture of tobacco, but its occurrence has been reported, for example, in primary tumors of potato tuber discs [34] . Similarly to Liu et al .…”

Section: Iaa Level In a Tumefaciens-transformed Tissue Culturescontrasting

confidence: 70%

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Auxin synthesis in Agrobacterium tumefaciens and A. tumefaciens-transformed plant tissue

Kutáček

Rovenská

1991

Plant Growth Regul

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Section: Iaa Level In a Tumefaciens-transformed Tissue Culturescontrasting

confidence: 70%

“…The indolepyruvate pathway was confirmed in the sunflower primary tumor [3] and in Nicotiana glauca and Nicotiana langsdorfii as well as in the genetic tumors occurring after their hybridization [20] .…”

Section: Iaa Level In a Tumefaciens-transformed Tissue Culturesmentioning

confidence: 99%

Auxin synthesis in Agrobacterium tumefaciens and A. tumefaciens-transformed plant tissue

Kutáček

Rovenská

1991

Plant Growth Regul

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“…Another way in which high levels of tryptamine might be diverted from IAA synthesis in pea roots is the formation of Nv-acetyltryptamine, detected here in large amounts after feeds of [ (Phelps and Sequeira, 1967), and to IAAld, IEt, and IAA in both tomato and barley (Schneider et al, 1970;Gibson et al, 1972). Furthermore, the conversion of [ 14 C]Trp to tryptamine was noted in Nicotiana callus (Liu et al, 1978) and terminal buds (Phelps and Sequeira, 1967), and also in tomato and barley shoots (Schneider et al, 1970). However, none of these studies used the definitive techniques of GC/MS.…”

Section: Discussionmentioning

confidence: 99%

Auxin Biosynthesis in Pea: Characterization of the Tryptamine Pathway

et al. 2009

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One pathway leading to the bioactive auxin, indole-3-acetic acid (IAA), is known as the tryptamine pathway, which is suggested to proceed in the sequence: tryptophan (Trp), tryptamine, N-hydroxytryptamine, indole-3-acetaldoxime, indole-3-acetaldehyde (IAAld), IAA. Recently, this pathway has been characterized by the YUCCA genes in Arabidopsis (Arabidopsis thaliana) and their homologs in other species. YUCCA is thought to be responsible for the conversion of tryptamine to N-hydroxytryptamine. Here we complement the genetic findings with a compound-based approach in pea (Pisum sativum), detecting potential precursors by gas chromatography/tandem-mass spectrometry. In addition, we have synthesized deuterated forms of many of the intermediates involved, and have used them to quantify the endogenous compounds, and to investigate their metabolic fates. Trp, tryptamine, IAAld, indole-3-ethanol, and IAA were detected as endogenous constituents, whereas indole-3-acetaldoxime and one of its products, indole-3-acetonitrile, were not detected. Metabolism experiments indicated that the tryptamine pathway to IAA in pea roots proceeds in the sequence: Trp, tryptamine, IAAld, IAA, with indole-3-ethanol as a side-branch product of IAAld. N-hydroxytryptamine was not detected, but we cannot exclude that it is an intermediate between tryptamine and IAAld, nor can we rule out the possibility of a Trp-independent pathway operating in pea roots.

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“…All possible pathways of the plant's own IAA biosynthesis, with either tryptamin, indole-3-pyruvic acid or IAOX as primary intermediate, would operate via IAALD which in vivo and in vitro may be reduced to IEOH by IAALD reductase (Bower et al 1978). IEOH has also been identified as a product of TRP metabolism in genetic tumors of tobacco (Liu et al 1978).…”

Section: Rf-value 8 12mentioning

confidence: 99%

Biosynthesis and conjugation of indole‐3‐acetic acid in tobacco crown gall

Rausch¹,

Kahl²,

Hilgenberg³

1986

Physiologia Plantarum

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The in vivo metabolism of [3H]‐tryptophan (TRP), [14C]‐indole‐3‐acetaldoxime (IAOX), and [14C]‐indole‐3‐acetic acid (IAA) has been studied in non‐transformed and Agrobacterium tumefaciens T 37 transformed cells of Nicotiana tabacum L. cv. White Burley. Metabolites were analyzed by thin‐layer chromatography (TLC) and high pressure liquid chromatography (HPLC). TRP was converted to IAOX and indole‐3‐ethanol (IEOH) in both cell types, while indole‐3‐acetamide (IAAM) occurred exclusively in transformed cells. The formation of IEOH was higher in transformed cells. IAOX was metabolized to IEOH and IAA in both tissues, whereas only in transformed cells some conversion to IAAM occurred. Furthermore, the conversion of the aldoxime to IAA was higher in transformed cells. Labelled IAA became rapidly conjugated to indole‐3‐acetylaspartic acid (IAAsp) in both cell types, the transformed cells showing a 100% increased uptake of IAA. In vitro incubations of [14C]‐IAOX with desalted extracts from transformed cells showed no significant enzymatic conversion to IAAM, whereas in the presence of flavin adenine dinucleotide (FAD) some chemical conversion was observed. The present results suggest that in an established line of transformed cells the integration of the T‐DNA leads to a permanent transcription of its own auxin genes. Furthermore, the in vivo studies suggest that the host cell's capacity for IAA biosynthesis and conjugation may be altered quantitatively. The potential role of IAOX in both transformed and non‐transformed cells is discussed.

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Indole-3-acetic Acid Synthesis in Tumorous and Nontumorous Species of Nicotiana

Abstract: The synthesis of indole-3-acetic acid (IAA)

Cited by 16 publications

References 12 publications

Auxin synthesis in Agrobacterium tumefaciens and A. tumefaciens-transformed plant tissue

Auxin synthesis in Agrobacterium tumefaciens and A. tumefaciens-transformed plant tissue

Auxin Biosynthesis in Pea: Characterization of the Tryptamine Pathway

Biosynthesis and conjugation of indole‐3‐acetic acid in tobacco crown gall

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