Indole-3-carbinol attenuates the deleterious gestational effects of bisphenol A exposure on the prostate gland of male F1 rats

Genistein reduces the noxious effects of in utero bisphenol A exposure on the rat prostate gland at weaning and in adulthood

“…Also, AR expression in the VP was higher on PND21 in the BPA 25-treated group (G2) compared to the untreated group. This result is in agreement with other rodent studies which indicates that prenatal or neonatal exposure of male rat and mice to low doses of estradiol and other estrogenic chemicals like BPA and offspring can lead to androgen sensitivity and consequently to an increased expression of AR in the prostate in pubertal and young adults rodents (Nagel et al, 1997;Gupta, 2000;Brandt et al, 2014). Furthermore, in a previous study we observed that estrogen supplementation even in a lowandrogen environment (after castration) increased AR expression in Mongolian gerbil ventral prostate through paracrine signaling mechanisms involving ER-a (Scarano et al, 2009).…”

“…In the GEN-treated groups, the conjunctive fibers appeared more dispersed but occurred in a higher proportion than that observed in the untreated group. It clear that the change in the pattern of collagen budding with reduction in relative proportion of stroma compartment caused by gestational BPA exposure resulted in changes in the distribution and aggregation of extracellular matrix elements (Brandt et al, 2014). Previous reports have shown that exposure to estradiol may increase the synthesis of collagen in the stromal cells and make collagen bundles thicker in rodent prostate (Tilley et al, 1989;Prins et al, 2007;Scarano et al, 2005Scarano et al, , 2008.…”

“…In fact, prenatal and neonatal BPA exposure can adversely affect the critical windows of tissue architecture and homeostasis, resulting in significant adverse effects late-in-life (Golub et al, 2010;Rochester, 2013). Also, an early in life exposure to BPA can interfere with the development of the prostate and induce metaplasia, hyperplasia, and inflammation in adulthood (Gupta, 2000;Ramos et al, 2001;Timms et al, 2005;Ogura et al, 2007;Brandt et al, 2014).…”

“…BPA was dissolved in DMSO and solubilized in canola oil (1% final DMSO volume) and two solutions were prepared at final concentrations of 5 mg/mLor 50 mg/mL of BPA. The first solution (5 mg/mL) was used in dams exposed to BPA at 25 mg/kg body weight; and the other solution (50 mg/mL) was used in dams exposed to BPA at250 mg/kg body weight (Brandt et al, 2014). During GD10 to 21, dams received basal diet or basal diet containing GEN (99% purity and gently donated by DSM Nutritional Products e Basel, Switzerland) at concentration of 250 mg/kg chow and drinking water ad libitum.…”

“…However, some studies have shown that environmentally relevant doses of BPA (<50 mg/kg/day) during pregnancy can result in morphological and functional prostate changes and in increased risk for prostate cancer development in both adult male rats and mice (Ramos et al, 2001;Ho et al, 2006;Ogura et al, 2007;Prins et al, 2008;Brandt et al, 2014). In addition, some studies have demonstrated that BPA is easily transferred across the placenta, indicating a potential risk for rat fetuses (Takahashi and Oishi, 2000;Doerge et al, 2011).…”