Indole affects the formation of multicellular aggregate structures in &lt;i&gt;Pantoea agglomerans&lt;/i&gt; YS19

Yu, Xin; Jiang, Jing; Chen, Liang; Zhang, Xiao; Wang, Jieru; Shen, Dejian; Feng, Yongjun

doi:10.2323/jgam.62.31

Cited by 21 publications

(18 citation statements)

References 34 publications

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“…The stresses from heavy metals or pH changes are often faced by endophytes (Zheng et al, 2017). In our previous work, the symplasmata of YS19 have been tested to enhance the stress-resistant ability to these chemicals (CuSO 4 and acid) in comparison with the planktonic cells (Yu et al, 2016). So, CuSO 4 and acid are selected for the stress-resistant experiments.…”

Section: Treatment Of Seeds*mentioning

confidence: 99%

Extracellular DNA enhances the formation and stability of symplasmata in <i>Pantoea agglomerans</i> YS19

Zheng

Xia

Liu

et al. 2019

J. Gen. Appl. Microbiol.

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Extracellular DNA (eDNA) is an important polymeric substance that plays essential roles in cell aggregation and nutrient provision for the sessile bacteria. eDNA in bacterial biofilms was extensively studied. Here we found that eDNA also exists in symplasmata, a bacterial cell aggregate, which is different to a biofilm, in the rice enophyte Pantoea agglomerans YS19. We found that exogenous eDNA enhanced the formation and stability of symplasmata significantly, and that, exogenous eDNA also improved the stress resistance and colonization ability of the bacterium on host rice. These results strongly indicate novel roles of the eDNA in Pantoea agglomerans YS19, showing its special relation to the stress-resistance and endophyte-host association of the strain.

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Section: Treatment Of Seeds*mentioning

confidence: 99%

Extracellular DNA enhances the formation and stability of symplasmata in <i>Pantoea agglomerans</i> YS19

Zheng

Xia

Liu

et al. 2019

J. Gen. Appl. Microbiol.

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“…The ecological role and evolutionary beginnings of symplasmata formation in Pantoea bacteria remain unclear. There have been reports that these structures may confer tolerance to stresses such as acids, heavy metals and UV 21 . For plant-colonizing P. agglomerans strains, symplasmata have been directly observed on the surfaces of roots and leaves, as well as within plant tissues 21 22 23 24 25 26 27 28 .…”

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confidence: 99%

Symplasmata are a clonal, conditional, and reversible type of bacterial multicellularity

Tecon

Leveau

2016

Sci Rep

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Microorganisms are capable of remarkable social behaviours, such as forming transient multicellular assemblages with properties and adaptive abilities exceeding those of individual cells. Here, we report on the formation and structure of genets known as symplasmata produced by Pantoea eucalypti bacteria. Each symplasmatum develops clonally and stochastically from a single bacterium into a membrane-delimited, capsule-embedded cluster of progeny cells and with a frequency that depends on temperature, pH, and nutrient availability. Transposon mutagenesis identified several gene products required for symplasmata formation, including master regulator LrhA, replication inhibitor CspD, polysaccharide transporter RfbX3, and autoinducer synthase PhzI. We also show that bacteria inside symplasmata are shaped irregularly with punctuated cell-to-cell contacts, metabolically responsive to environmental stimuli, dispersal-ready, and transcriptionally reprogrammed to anticipate multiple alternative futures in terms of carbon source availability. The structured and conditionable nature of symplasmata offers exciting prospects towards a mechanistic understanding of multicellular behaviours and their ecological significance.

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“…The fact of a positive effect of D-Tyr on symplasmata formation while a negative effect on biofilm formation suggests that the two structures most likely have different formation mechanism and regulation pathway, that is, they are different kinds of cell aggregates and symplasmata are independent from biofilms. Interestingly, another very recent study from us also showed that a signal molecule indole promotes symplasmata yet inhibits biofilm formation in YS19 [9].…”

Section: Discussionmentioning

confidence: 86%

“…Kinetics of cell growth, symplasmata, and biofilm formation Determination of cell growth was carried out by monitoring bacterial dry weight of YS19 cells at different growth times as described [2,9]. D-Tyr (final concentration 3, 6, 15, and 30 mM) was added to the experimental groups.…”

Section: D-amino Acid Solution Preparationmentioning

confidence: 99%

D-tyrosine affects aggregation behavior ofPantoea agglomerans

Yang

Jiang

et al. 2016

J. Basic Microbiol.

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D-amino acids have been proved to disassemble biofilms by disassociating the matrix. Pantoea agglomerans is characterized by the formation of another kind of multicellular structure called symplasmata, which also remains the ability to form biofilms. In this study, a rice diazotrophic endophyte P. agglomerans YS19 was selected as a model strain to explore the effects of D-amino acids on these two kinds of cell aggregate structures. It was discovered that D-tyrosine disassociates biofilm, yet promotes symplasmata formation. D-tyrosine showed no influence on bacterial growth yet promoted the bacterial motility and inhibited the expression of cellular MalE and OmpF proteins, which enriched our knowledge of the biological effect of D-amino acids and expanded the research ideas of symplasmata formation.

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Indole affects the formation of multicellular aggregate structures in <i>Pantoea agglomerans</i> YS19

Cited by 21 publications

References 34 publications

Extracellular DNA enhances the formation and stability of symplasmata in <i>Pantoea agglomerans</i> YS19

Extracellular DNA enhances the formation and stability of symplasmata in <i>Pantoea agglomerans</i> YS19

Symplasmata are a clonal, conditional, and reversible type of bacterial multicellularity

D-tyrosine affects aggregation behavior ofPantoea agglomerans

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