“…U-251 MG is an established GBM cell line [ 15 ], part of the NCI-60 human cancer cell lines [ 25 ], with the ability to form spheroids with invasive properties [ 26 ]; it has been used in the screening of pharmaceutical agents to identify, characterize, and select novel compounds with growth inhibition and/or killing of tumor cells. A series of 22 different compounds called BS, BSK, JVM PNR, and ST [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ] whose the chemical structures, details, and activity are explained in Table 1 and Figure S1 , were incubated with the spheroids for 48 h to define the toxicity the effects on their invasiveness. These compounds have anti-invasive properties, as they interfere with pathways of cell invasion ( Figure 2 ).…”