Indoleamine-2,3-dioxygenase-1 is a molecular target for the protective activity of mood stabilizers against mania-like behavior induced by d-amphetamine

Tran, Hai Quyen; Shin, Eun‐Joo; Tran, The-Vinh; Phan, Hien; Sharma, Naveen; Kim, Dae‐Won; Choi, Soo Young; Jeong, Ji Hoon; Jang, Choon‐Gon; Cheong, Jae Hoon; Nabeshima, Toshitaka; Kim, Hyoung‐Chun

doi:10.1016/j.fct.2019.110986

Cited by 11 publications

(11 citation statements)

References 93 publications

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“…The energized mitochondria were then incubated with 10 µM JC-1 for 30 min at 37 • C, and fluorescence was measured using a fluorescence plate reader (Molecular Devices Inc., Sunnyvale, CA, USA). Mitochondrial polarization was measured by taking the emission ratio from 590 nm to 535 nm with excitation at 490 nm [13,[105][106][107][108]…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Rotenone (1 µM) was then added, and the absorbance was measured again at 600 nm for 4 min at intervals of 60 s. One unit of complex I activity is defined as 1 µmoL 2,6-dichloroindophenol reduced per minute, and it was calculated based on the extinction coefficient for 2,6-dichloroindophenol (19.1 mM −1 cm −1 ). The results have been expressed as a percentage of the control group [10,108,111].…”

Section: Measurement Of Complex I Activitymentioning

confidence: 99%

“…One unit of complex II activity is defined as the reduction of 1 µmol 2,6-dichloroindophenol per minute. Calculation of activity was based on the extinction coefficient of 2,6-dichloroindophenol (19.1 mM −1 cm −1 ) [10,108,111].…”

Section: Measurement Of Mitochondrial Complex II Activitymentioning

confidence: 99%

“…Protein oxidation was determined by analyzing the content of protein carbonyl groups using a 2,4-dinitrophenylhydrazine (DNPH)-labeling procedure [113]. DNPH-labeled protein was detected using a microplate reader [10,13,104,108], and the results were represented as nmol of DNPH incorporated/mg protein based on the extinction coefficient for aliphatic hydrazones (21 mM −1 cm −1 ). BCA Protein Assay kit (Thermo Scientific) was used to measure protein concentration.…”

Section: Determination Of Protein Carbonylmentioning

confidence: 99%

“…This reduced form of Rhod-2-AM is a colorless, non-fluorescent dye with a net positive charge, which promotes sequestration into the mitochondria, whereas dye oxidized in the mitochondria and cleaved AM ester are trapped inside the mitochondria. Fluorescence plate reader (Molecular Devices Inc.) was used to measure fluorescence with an excitation wavelength of 549 nm and emission wavelengths of 581 nm[13,[108][109][110].…”

mentioning

confidence: 99%

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Ginsenoside Re Protects against Serotonergic Behaviors Evoked by 2,5-Dimethoxy-4-iodo-amphetamine in Mice via Inhibition of PKCδ-Mediated Mitochondrial Dysfunction

Shin

Jeong

Nguyen

et al. 2021

IJMS

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It has been recognized that serotonin 2A receptor (5-HT2A) agonist 2,5-dimethoxy-4-iodo-amphetamine (DOI) impairs serotonergic homeostasis. However, the mechanism of DOI-induced serotonergic behaviors remains to be explored. Moreover, little is known about therapeutic interventions against serotonin syndrome, although evidence suggests that ginseng might possess modulating effects on the serotonin system. As ginsenoside Re (GRe) is well-known as a novel antioxidant in the nervous system, we investigated whether GRe modulates 5-HT2A receptor agonist DOI-induced serotonin impairments. We proposed that protein kinase Cδ (PKCδ) mediates serotonergic impairments. Treatment with GRe or 5-HT2A receptor antagonist MDL11939 significantly attenuated DOI-induced serotonergic behaviors (i.e., overall serotonergic syndrome behaviors, head twitch response, hyperthermia) by inhibiting mitochondrial translocation of PKCδ, reducing mitochondrial glutathione peroxidase activity, mitochondrial dysfunction, and mitochondrial oxidative stress in wild-type mice. These attenuations were in line with those observed upon PKCδ inhibition (i.e., pharmacologic inhibitor rottlerin or PKCδ knockout mice). Furthermore, GRe was not further implicated in attenuation mediated by PKCδ knockout in mice. Our results suggest that PKCδ is a therapeutic target for GRe against serotonergic behaviors induced by DOI.

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Section: Western Blot Analysismentioning

confidence: 99%

Section: Measurement Of Complex I Activitymentioning

confidence: 99%

Section: Measurement Of Mitochondrial Complex II Activitymentioning

confidence: 99%

Section: Determination Of Protein Carbonylmentioning

confidence: 99%

mentioning

confidence: 99%

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Ginsenoside Re Protects against Serotonergic Behaviors Evoked by 2,5-Dimethoxy-4-iodo-amphetamine in Mice via Inhibition of PKCδ-Mediated Mitochondrial Dysfunction

Shin

Jeong

Nguyen

et al. 2021

IJMS

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Ginsenoside Rb3 Promotes Opa1‐Mediated Regenerative Neurogenesis via Activating the Ido1 Pathway in Ischemic Stroke

Wang,

Qin,

Gao

et al. 2024

Phytotherapy Research

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The activation of neural stem cells (NSCs) residing in the subventricular zone (SVZ) and dentate gyrus (DG) has been shown to promote the restoration of damaged brain tissues. Ginsenoside Rb3 (Rb3) is a bioactive substance known for its pharmacological properties in treating neurological disorders. This study investigated the effects of Rb3 on neural regeneration following ischaemic stroke (IS) and the underlying mechanisms involved. Male C57BL/6 mice were utilized and were subjected to middle cerebral artery occlusion/reperfusion (MCAO/R). Post‐ischemia, Rb3 was administered through intraperitoneal (i.p.) injection for either 7 or 28 days. The promotion of Rb3 on regenerative neurogenesis was detected by immunofluorescence staining. NSCs were pretreated with different concentrations of Rb3 for 24 h before oxygen–glucose deprivation/reoxygenation (OGD/R) exposure. Afterward, immunofluorescence staining and flow cytometry were used to detect the migration and proliferation of Rb3 in OGD/R‐induced NSCs. Furthermore, Adeno‐associated virus (AAV) transduction experiments, siRNA transfection experiments, gene knockout experiments, targeted metabolomics analysis, molecular dynamics simulation, cellular thermal shift assay (CETSA), and drug affinity responsive target stability (DARTS) assays were used to explore the promotion and mechanism of Rb3 on regenerative neurogenesis following IS. Rb3 promoted Opa1‐mediated NSCs migration and proliferation. Knockdown of Opa1 blunted the above‐promoting effects of Rb3 in both the brains of ischemia–reperfusion (I/R)‐treated mice and OGD/R‐treated NSCs. Mechanistically, targeted metabolomics, molecular dynamics, molecular docking, CETAS, and DARTS experiments showed that Rb3 promoted Opa1‐mediated neural regeneration required the activation of Ido1 and that Ido1 served as a direct target of Rb3 to repair I/R injury. Moreover, studies in siRNA‐mediated knockdown and KO mice revealed that inhibition of Ido1 attenuated the enhancing effect of Rb3 on mitochondrial fusion. Our study provides novel evidence that Rb3 promotes neurogenesis through an Ido1/Opa1‐mediated pathway involving the interaction between Rb3 and Ido1, leading to improved long‐term neurological function. These results indicate that Rb3 or other mitochondrial fusion promoters could be a potential neurorestorative strategy for regenerative neurogenesis following IS.

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The dopamine transporter inhibition using GBR 12909 as a novel pharmacological tool to assess bipolar disorder-like neurobehavioral phenotypes in zebrafish

Canzian,

Borba,

Resmim

et al. 2025

Behavioural Brain Research

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Indoleamine-2,3-dioxygenase-1 is a molecular target for the protective activity of mood stabilizers against mania-like behavior induced by d-amphetamine

Cited by 11 publications

References 93 publications

Ginsenoside Re Protects against Serotonergic Behaviors Evoked by 2,5-Dimethoxy-4-iodo-amphetamine in Mice via Inhibition of PKCδ-Mediated Mitochondrial Dysfunction

Ginsenoside Re Protects against Serotonergic Behaviors Evoked by 2,5-Dimethoxy-4-iodo-amphetamine in Mice via Inhibition of PKCδ-Mediated Mitochondrial Dysfunction

Ginsenoside Rb3 Promotes Opa1‐Mediated Regenerative Neurogenesis via Activating the Ido1 Pathway in Ischemic Stroke

The dopamine transporter inhibition using GBR 12909 as a novel pharmacological tool to assess bipolar disorder-like neurobehavioral phenotypes in zebrafish

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