Indomethacin and Ibuprofen Effect on IL-1ra Production by Mononuclear Cells of Preterm Newborns and Adults

Bessler, Hanna; Ziyada, S; Bergman, Michael; Punsky, Igor; Sirota, Lea

doi:10.1159/000063090

Cited by 5 publications

(3 citation statements)

References 40 publications

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“…Inflammatory reaction is an essential component of the body defence mechanism. Bessler et al (2002) have reported that 20 µg/ml of IB added to LPS-stimulated PBMC from healthy adults had no effect on IL-1ra secretion. However, at concentrations of 100 and 200 µg/ml, production of this cytokine was significantly suppressed by 11 % and 54 %, respectively.…”

Section: Discussionmentioning

confidence: 97%

The Effect of Ibuprofen on Cytokine Production by Mononuclear Cells from Schizophrenic Patients

Bessler,

Cohen-Terica,

Djaldetti

et al. 2017

Fol. Biol.

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The existence of a restrained inflammatory state in schizophrenic individuals posed the question whether anti-inflammatory drugs may exert antipsychotic effects. Therefore, the effect of ibuprofen (IB) on cytokine production by human peripheral blood mononuclear cells (PBMC) from schizophrenic patients was examined and compared to that of healthy subjects. PBMC from 25 schizophrenic patients and 24 healthy volunteers were incubated for 24 h with lipopolysaccharide (LPS) in the absence or presence of various concentrations of IB. The levels of IL-1β, IL-6, TNF-α, IL-10 and IL-1ra in the supernatants were tested applying ELISA kits. The secretion of TNF-α by cells from schizophrenic patients was significantly lower compared with controls. IB caused stimulation of TNF-α and IL-6 production by cells of the two groups and enhanced IL-1β secretion by cells from schizophrenic patients. IB inhibited IL-1ra and IL-10 generation by cells from the two groups. Without IB, IL-1ra secretion was negatively correlated with the disease severity, while 200 μg/ml of IB positively correlated with the PANSS total score. IL-10 production was positively correlated with the PANSS positive subscale score both in the absence or presence of IB. The findings suggest that the effect of IB on the production of inflammatory cytokines may benefit the health of schizophrenic patients.

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Section: Discussionmentioning

confidence: 97%

The Effect of Ibuprofen on Cytokine Production by Mononuclear Cells from Schizophrenic Patients

Bessler,

Cohen-Terica,

Djaldetti

et al. 2017

Fol. Biol.

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“…However, it has been shown that the capacity of mononuclear cells from preterm newborns to produce IL-1ra and IL-1ß is lower than that of adult cells [22][23][24][25][26][27][28], a phenomenon that could be attributed to the immaturity of the preterms' immune system. It is therefore suggested that the lack of an association between IL-1ra gene polymorphism and the risk of sepsis in preterm neonates may be related to the lower capacity of the neonatal cells to produce IL1ra and IL-1ß, cytokines important in controlling the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Association between IL-1ra Gene Polymorphism and Premature Delivery

Bessler

Osovsky²,

Sirota³

2004

Neonatology

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IL-1 receptor antagonist (IL-1ra) gene polymorphism was examined in 95 Israeli preterm newborns and compared to that of adult volunteers. The genotype was determined using PCR amplification of the variable region of intron 2 of the IL-1ra gene. The IL-1raA1 allele was found to be predominant in the two groups. However, a significant higher frequency of IL-1raA2 allele was found in preterm newborns. The difference was mainly due to higher proportion of homozygous for IL-1raA2 in the preterm neonates (19%) as compared with adults (7%). No such association could be demonstrated between IL-1raA2 allele and severe sepsis in preterm newborns. The frequency of IL-1raA2 allele among preterms with a septic episode did not differ significantly from that found in newborns without sepsis. The results suggest an association between the IL-1ra genotype and the incidence of premature delivery.

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“…Due to low volumes of cord blood available in the present work, PBMCs from adults were used to obtain sufficient amounts of mononuclear cells. Since CBMCs are immature and may therefore respond differently to exposure than PBMCs from adults (Bessler et al, 2002;Bessler et al, 2007;Goldberg et al, 2008), the possibility of different effects of exposure to the 12 investigated substances for the CBMCs and PBMCs cannot be excluded.…”

Section: In Vitro Assaymentioning

confidence: 99%

In vitro cytokine release from human peripheral blood mononuclear cells in the assessment of the immunotoxic potential of chemicals

Stølevik

Nygaard

Granum

et al. 2011

Toxicology in Vitro

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Alternative methods to the use of animals in testing of chemicals are needed. We investigated if the immunotoxic potential of 12 dietary toxicants could be predicted from effects on cytokine release from human peripheral blood mononuclear cells (PBMC) after in vitro exposure. Nine cytokines were selected to reflect different types of immune responses. The toxicants were classified as immunotoxic or non-immunotoxic substances according to the published in vivo data. Isolated human PBMC were exposed for 20 h to three concentrations of each of the 12 substances in the presence of human liver S9 fraction. After further incubation of PBMC in fresh medium containing the mitogen phytohemagglutinin (PHA, 10 μg/ml) for 48 h, release of the nine selected cytokines into the supernatant as well as cell proliferation were measured by Luminex technology™ and the BrdU incorporation assay, respectively. All 12 substances investigated affected the release of one or more cytokines, and each of the substances showed different cytokine release patterns. Within the limitations of the study design, the present study suggests that the effect of the substances on mitogen-induced cytokine release from PBMC cannot predict their immunotoxic potential, but may be useful in mechanistic studies.

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Indomethacin and Ibuprofen Effect on IL-1ra Production by Mononuclear Cells of Preterm Newborns and Adults

Cited by 5 publications

References 40 publications

The Effect of Ibuprofen on Cytokine Production by Mononuclear Cells from Schizophrenic Patients

The Effect of Ibuprofen on Cytokine Production by Mononuclear Cells from Schizophrenic Patients

Association between IL-1ra Gene Polymorphism and Premature Delivery

In vitro cytokine release from human peripheral blood mononuclear cells in the assessment of the immunotoxic potential of chemicals

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