Indomethacin does not alter natural killer cell response to 2.5 h of running

Abstract

The effect of 2.5 h of treadmill running at 75.6 +/- 0.9% maximal O2 uptake (VO2max) on natural killer (NK) cell cytotoxic activity (NKCA) was investigated in 22 experienced marathon runners (VO2max 57.9 +/- 1.1 ml.kg-1.min-1, age 38.7 +/- 1.5 yr). Blood samples were taken before (0715) and immediately after exercise (1000), with three more samples taken during 6 h of recovery (1130, 1300, and 1600). Ten sedentary controls (VO2max 34.7 +/- 1.0 ml.kg-1.min-1, age 45.3 +/- 2.3 yr) sat in the laboratory during testing and had their blood sampled at the same time points. The pattern of change in NKCA over time was significantly different between groups [F(4,27) = 6.53; P = 0.001], with the runner's NKCA dropping 51-61% below preexercise levels throughout 6 h of recovery. Preincubation of blood mononuclear cells in vitro with indomethacin had no effect on the difference in pattern of change in NKCA between groups [F(4,17) = 8.59; P = 0.001] and did not attenuate the postexercise reduction in the runners. When NKCA was adjusted on a per-NK cell basis, group differences and the postexercise decline in NKCA were eliminated [F(4,80) = 0.65; P = 0.63]. Serum cortisol and plasma epinephrine in the runners were elevated relative to control subjects during recovery from exercise, but no significant correlation with changes in NK cells or NKCA was found. These data indicate that NKCA is decreased significantly during recovery from 2.5 h of running due to a numerical redistribution of NK cells.

Carbohydrate Supplementation and Immune Responses after Acute Exhaustive Resistance Exercise

“…Concurrent with several reports Mitchell et al, 1998;Nieman, Simandle, et al, 1995;Simonson & Jackson, 2004), we observed increases in total leukocyte, lymphocytes, and neutrophils immediately after acute resistive exercise. We did not, however, observe the same magnitude of cell responses with resistive exercise as that reported during endurance exercise.…”

“…Acute bouts of heavy endurance or resistance exercise have been shown to influence immune cells in the circulating peripheral blood (Henson et al, 1999(Henson et al, , 1998Kraemer et al, 1996;Nieman, 1997;Nieman, Simandle, et al, 1995;Simonson & Jackson, 2004■). Concurrent with several reports Mitchell et al, 1998;Nieman, Simandle, et al, 1995;Simonson & Jackson, 2004), we observed increases in total leukocyte, lymphocytes, and neutrophils immediately after acute resistive exercise.…”

“…A Newman-Keuls post hoc analysis was used to isolate differences among treatment means. Previous studies of endurance athletes (Henson et al, 1998;Nieman, Simandle, et al, 1995) have reported attenuation of immune responses of up to 25-50% with CHO supplementation. Based on this observation, we assumed that a similar change could be expected in the current study and would be considered meaningful.…”

“…Furthermore, plasma glucose and cortisol levels were equally elevated after resistance exercise with both carbohydrate and placebo treatments. Carbohydrate ingestion has been shown to attenuate cortisol responses with exercise of 75-80% VO 2max and 2.5 hr of running (Henson et al, 1998;Nieman, Simandle, et al, 1995). Haff et al (2000) found that an acute bout of resistance exercise lasting approximately 39 min decreased muscle glycogen content of the vastus lateralis by 26.7% compared with carbohydrate (CHO) supplementation (13.7% decline).…”

“…Given previous findings (Henson et al, 1998;Koch et al, 2001;Nieman, Simandle, et al, 1995), the aim of our investigation was to study changes in leukocyte subsets after an acute session of resistance exercise and to further determine whether the ingestion of CHO would attenuate those immune responses. We hypothesized that ingestion of a CHO beverage would attenuate the cortisol response and prevent subsequent lymphocyte reductions after resistance exercise.…”