Indoor Air Pollution and Sick Building Syndrome. Monitoring Aerosol Protein as a Measure of Bioaerosols

Poruthoor, Simon K.; Dasgupta, Purnendu Κ.; Zhang, Genfa

doi:10.1021/es9710058

Cited by 14 publications

(8 citation statements)

References 24 publications

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“…c Calculated as the difference between TSP and PM 10 ; d These values represent either 1 / 2 LOD or 1 / 2 propagation errors. for protein (Poruthoor et al 1998;Miguel et al 1999;Menetrez et al 2001), for (1→3)-β-D-glucan (Thorn and Rylander 1998;Wan and Li 1999;Beijer et al 2002;Rylander and Beijer 2003;Lee et al 2006;Miller et al 2007), and for endotoxin (Wan and Li 1999;Park et al 2000Park et al , 2001Rylander and Beijer 2003;Kujundzic et al 2006;Miller et al 2007). However, our endotoxin concentrations were substantially less than the 17-139 EU/m 3 found in 3 homes in New Orleans after Hurricane Katrina (Chew et al 2006).…”

Section: The Characteristics Of Indoor Bioaerosolsmentioning

confidence: 99%

“…Protein, a polymer of amino acids, exists in all biological materials and consequently can be used to reflect overall bioaerosol concentrations (Poruthoor et al 1998;Borodulin et al 2005). Endotoxin, a lipopolysaccharide cell wall component of the outer membrane of gram-negative bacteria (GNB), can be used to assess the GNB content of particulate matter (PM) samples.…”

Section: Introductionmentioning

confidence: 99%

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Size-Resolved Concentrations of Particulate Matter and Bioaerosols Inside versus Outside of Homes

Chen

Hildemann

2009

Aerosol Science and Technology

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Size-resolved airborne particulate matter samples (PM 2.5 , PM 10 , and TSP) collected inside ten northern California homes over four days and one night (9-12 h/sample, spanning a 3.5 week period) were analyzed for protein, endotoxin, and (1→3)-β-D-glucan concentrations. Some simultaneous size-resolved outdoor samples were also collected. The associations of residential characteristics and occupant behavior with the indoor airborne levels were investigated. In addition, the relation between these chemical biomarkers and the more traditional culturing approaches was studied. Most of the indoor mass concentration of airborne particles and protein was in the fine fraction (PM 2.5 ), while the mass of airborne endotoxin and (1→3)-β-D-glucan was mainly in the coarser fractions (PM 10-2.5 and PM TSP-10 ). No strong correlations were seen between short-term (3-6 min) culturable bacteria and fungi counts and the corresponding longer-term (9-12 h) biomarker levels. Daytime indoor levels of the biomarkers tended to be higher than outdoors, especially for the PM 10-2.5 fraction, but only in a few cases were the indoor/outdoor relationships statistically significant. Indoor pets were associated with elevated airborne PM and bioaerosols inside homes. Two other factors, wall-to-wall carpet and older houses, also appear to be associated with some elevations in indoor levels.

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Section: The Characteristics Of Indoor Bioaerosolsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Size-Resolved Concentrations of Particulate Matter and Bioaerosols Inside versus Outside of Homes

Chen

Hildemann

2009

Aerosol Science and Technology

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“…Amino acids and proteins, both important constituents of bioaerosol, can be an index of its occurrence in atmosphere (Poruthoor et al, 1998;Czerwieniec et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Free and combined amino acids in size-segregated atmospheric aerosol samples

Filippo

Pomata

Riccardi

et al. 2014

Atmospheric Environment

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“…Furthermore, according to Bauer et al fungal spores can be regarded as primary components of PM 10 , total organic carbon (OC), and, most importantly, coarse OC even in urban areas . Fungi are indispensable in nature for biodegradation and are beneficial to humans in terms of food production, medicine, and so forth. − However, a number of fungi are major pathogens/allergens for humans, animals, and plants and have significant adverse health effects as well as economic impacts. ,− For instance, fungi are potentially important contributors to indoor air pollution and have been consistently implicated in building-related illnesses and symptoms. − In 2009 the World Health Organization concluded that sufficient epidemiological evidence was available to show that occupants of damp or moldy buildings are at increased risk of respiratory symptoms, respiratory infections, and asthma exacerbation . This was further confirmed by a subsequent review .…”

Section: Introductionmentioning

confidence: 99%

Raman Microspectroscopy-Based Identification of Individual Fungal Spores as Potential Indicators of Indoor Contamination and Moisture-Related Building Damage

Ghosal

Macher

Ahmed

2012

Environ. Sci. Technol.

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We present an application of Raman microspectroscopy (RMS) for the rapid characterization and identification of individual spores from several species of microfungi. The RMS-based methodology requires minimal sample preparation and small sample volumes for analyses. Hence, it is suitable for preserving sample integrity while providing micrometer-scale spatial resolution required for the characterization of individual cells. We present the acquisition of unique Raman spectral signatures from intact fungal spores dispersed on commercially available aluminum foil substrate. The RMS-based method has been used to compile a reference library of Raman spectra from several species of microfungi typically associated with damp indoor environments. The acquired reference spectral library has subsequently been used to identify individual microfungal spores through direct comparison of the spore Raman spectra with the reference spectral signatures in the library. Moreover, the distinct peak structures of Raman spectra provide detailed insight into the overall chemical composition of spores. We anticipate potential application of this methodology in the fields of public health, forensic sciences, and environmental microbiology.

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Indoor Air Pollution and Sick Building Syndrome. Monitoring Aerosol Protein as a Measure of Bioaerosols

Cited by 14 publications

References 24 publications

Size-Resolved Concentrations of Particulate Matter and Bioaerosols Inside versus Outside of Homes

Size-Resolved Concentrations of Particulate Matter and Bioaerosols Inside versus Outside of Homes

Free and combined amino acids in size-segregated atmospheric aerosol samples

Raman Microspectroscopy-Based Identification of Individual Fungal Spores as Potential Indicators of Indoor Contamination and Moisture-Related Building Damage

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