Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor‐α

Dinh, Nhung Thi Hong; Lee, Jae‐Wook; Lee, Jaemin; Kim, Sang Soo; Go, Gyeongyun; Bae, Seoyoon; Jun, Ye In; Yoon, Yae Jin; Roh, Tae-Young; Gho, Yong Song

doi:10.1080/20013078.2020.1766821

Cited by 14 publications

(14 citation statements)

References 41 publications

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“…The existence of EVs in the environmental media is receiving great attention. EVs were found in indoor dust. , For other types of environmental matrix, the concentration of EVs (∼10 5 –10 6 vesicles mL –1 ) comparable to that of bacterial cells was reported in open ocean sea water . EVs formed the components of wastewater effluent as well, with the levels at 2 × 10 6 mL –1 .…”

Section: Discussionmentioning

confidence: 80%

“…One study showed that indoor dust EVs from a dormitory building, mainly from Pseudomonas, can enhance melanoma lung metastasis in a dose-dependent manner in mice. 17 Further, pathogen-derived EVs were abundantly found in body fluids and tissue biopsies of human patients in vivo studies. 40−42 In our study, a total of 57 potential pathogens were identified as potential hosts of indoor EVs.…”

Section: Discussionmentioning

confidence: 99%

“…Extraction of EVs from the dust samples was processed according to a previous study. 17 Briefly, indoor dust was incubated in phosphate-buffered saline (PBS) in a ratio of 1: 25 (weight: volume) under a rotation speed of 180 rpm for 12 h at 4 °C. A gauze of 75 μm thickness was employed to filter insoluble matters.…”

Section: Methodsmentioning

confidence: 99%

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Widespread of Potential Pathogen-Derived Extracellular Vesicles Carrying Antibiotic Resistance Genes in Indoor Dust

Qin

Guo

Huang

et al. 2022

Environ. Sci. Technol.

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Extracellular vesicles (EVs) are newly recognized as important vectors for carrying and spreading antibiotic resistance genes (ARGs). However, the ARGs harbored by EVs in ambient environments and the transfer potential are still unclear. In this study, the prevalence of ARGs and mobile genetic elements (MGEs) in EVs and their microbial origins were studied in indoor dust from restaurants, kindergarten, dormitories, and vehicles. The amount of EVs ranged from 3.40 × 107 to 1.09 × 1011 particles/g dust. The length of EV-associated DNA fragments was between 21 bp and 9.7 kb. Metagenomic sequencing showed that a total of 241 antibiotic ARG subtypes encoding resistance to 16 common classes were detected in the EVs from all four fields. Multidrug, quinolone, and macrolide resistance genes were the dominant types. 15 ARG subtypes were exclusively carried and even enriched in EVs compared to the indoor microbiome. Moreover, several ARGs showed co-occurrence with MGEs. The EVs showed distinct taxonomic composition with their original dust microbiota. 30.23% of EV-associated DNA was predicted to originate from potential pathogens. Our results indicated the widespread of EVs carrying ARGs and virulence genes in daily life indoor dust, provided new insights into the status of extracellular DNA, and raised risk concerns on their gene transfer potential.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Widespread of Potential Pathogen-Derived Extracellular Vesicles Carrying Antibiotic Resistance Genes in Indoor Dust

Qin

Guo

Huang

et al. 2022

Environ. Sci. Technol.

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“…Being a non-invasive and label-free technique with relatively high accuracy, DLS is often used along with flow cytometry as a complement for vesicle sizing or as validation for particles sizes acquired with NTA [ 69 ]. For instance, such complementary approaches were used to study human adipose-derived stem cell EVs on delaying cartilage degeneration, as well as examining the promotion of cancer lung metastasis upon treatment with indoor dust EVs [ 65 , 95 ].…”

Section: Particle Counting Sizing and Morphology Characterizationmentioning

confidence: 99%

Recent advances in optical label-free characterization of extracellular vesicles

et al. 2022

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Extracellular vesicles (EVs) are complex biological nanoparticles endogenously secreted by all eukaryotic cells. EVs carry a specific molecular cargo of proteins, lipids, and nucleic acids derived from cells of origin and play a significant role in the physiology and pathology of cells, organs, and organisms. Upon release, they may be found in different body fluids that can be easily accessed via noninvasive methodologies. Due to the unique information encoded in their molecular cargo, they may reflect the state of the parent cell and therefore EVs are recognized as a rich source of biomarkers for early diagnostics involving liquid biopsy. However, body fluids contain a mixture of EVs released by different types of healthy and diseased cells, making the detection of the EVs of interest very challenging. Recent research efforts have been focused on the detection and characterization of diagnostically relevant subpopulations of EVs, with emphasis on label-free methods that simplify sample preparation and are free of interfering signals. Therefore, in this paper, we review the recent progress of the label-free optical methods employed for the detection, counting, and morphological and chemical characterization of EVs. We will first briefly discuss the biology and functions of EVs, and then introduce different optical label-free techniques for rapid, precise, and nondestructive characterization of EVs such as nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy, surface plasmon resonance spectroscopy, Raman spectroscopy, and SERS spectroscopy. In the end, we will discuss their applications in the detection of neurodegenerative diseases and cancer and provide an outlook on the future impact and challenges of these technologies to the field of liquid biopsy via EVs.

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“…In the field studies, EVs were isolated from seawater using density gradient centrifugation, and these EVs carry varied types of cargoes in marine ecosystems (Biller et al., 2017 , 2014 ). EVs were also found in indoor dust and showed potential promoting effects on lung metastasis (Dinh et al., 2020 ). In our previous study, we found the prevalence of ARGs and mobile genetic elements (MGEs) in indoor dust EVs and their microbial origins were studied in indoor dust by metagenomic sequencing (Qin et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Diverse functional genes harboured in extracellular vesicles from environmental and human microbiota

Zhu

Huang

Avellán-Llaguno

et al. 2022

J of Extracellular Vesicle

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Exchange of mobile functional genes within microbiota benefits the microbial community. However, the status of the mobile gene pool in environment is still largely unclear, impeding the understanding on the process of gene transfer in natural microbial communities. The release of extracellular vesicles (EVs) by diverse organisms has been proposed to be a vital way in the complex networks of interactions between microbes and their habitats. In this study, we hypothesized that microbial EVs encapsulating functional DNA are widely distributed in the environmental matrix. The prevalence, source and DNA cargoes of EVs in three types of typical microbial habitats were studied. High abundance of EVs comparable to the bacterial concentration was found in human faeces, wastewater and soil. Metagenomic analysis showed the diverse and differential taxonomy of EVs‐associated DNA compared to source microbiome. An array of efficient EVs producing species was identified. A wide variety of mobile genes including glycoside hydrolase family 25 were enriched. Antibiotic resistance genes co‐localizing with mobile genetic elements were abundant in the EVs. This study provides novel insights into the prevalent EVs as a reservoir for the mobile functional genes in the natural environment.

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Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor‐α

Cited by 14 publications

References 41 publications

Widespread of Potential Pathogen-Derived Extracellular Vesicles Carrying Antibiotic Resistance Genes in Indoor Dust

Widespread of Potential Pathogen-Derived Extracellular Vesicles Carrying Antibiotic Resistance Genes in Indoor Dust

Recent advances in optical label-free characterization of extracellular vesicles

Diverse functional genes harboured in extracellular vesicles from environmental and human microbiota

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