Indoxacarb‐loaded fluorescent mesoporous silica nanoparticles for effective control of <i>Plutella xylostella</i> L. with decreased detoxification enzymes activities

Bilal, Muhammad; Xu, Chunli; Cao, Lidong; Zhao, Pengyue; Cao, Chong; Li, Fengmin; Huang, Qiliang

doi:10.1002/ps.5924

Cited by 42 publications

(32 citation statements)

References 73 publications

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“…Insects detoxify the different numerous exogenous and endogenous compounds due to the production of detoxification enzymes, including GSTs, CarE, and Cyt-b5, and their activities are considered to be an effective indicator monitoring the development of insect resistance to pesticides ( Li et al, 2021 ; Bilal et al, 2020 ). The presence of insecticides is reported to stimulate the activities of detoxification enzymes due to the increased production of ROS ( Jiang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Responses of Fungi Maggot (Bradysia impatiens Johannsen) to Allyl Isothiocyanate and High CO2

et al. 2022

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Botanical pesticide is highly recommended for integrated pest management (IPM), due to its merits such as environmental friendliness, safe to non-target organisms, operators, animals, and food consumers. The experiment was conducted to determine the lethal and sub-lethal effects of allyl isothiocyanate (AITC) on eggs, third instar larvae, pupae, and females and males of Bradysia impatiens Johannsen (B. impatiens). Different concentrations of AITC under ambient CO2 by the conical flask sealed fumigation method were used for the experiment. The results showed that there was a significant linear relationship between different concentrations of AITC and the toxicity regression equation of B. impatiens. The sub-lethal concentrations of AITC had significant effects on the larval stage, pupal stage, pupation rate, pupal weight, adult emergence rate, and oviposition. The pupation rate, pupal weight, and adult emergency rate were significantly (p < 0.05) affected by AITC fumigation. The pupation rate was the lowest after fumigation treatment of AITC at LC50 (36.67%), followed by LC25 (41.94%), compared with the CK (81.39%). Female longevity was significantly (p < 0.05) shortened by fumigation at LC25 (1.75 d) and LC50 (1.64 d), compared with that of CK (2.94 d). Male longevity was shorter at LC25 (1.56 d) than at LC50 (1.25 d) and had no significant difference between these two treatments. The fumigation efficiency of AITC was significantly increased under high CO2 condition. Furthermore, detoxification enzyme activities and antioxidant enzyme activities were accumulated under high CO2 condition. The fumigation method in the application of AITC can be useful in areas where B. impatiens is a major concern.

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Section: Discussionmentioning

confidence: 99%

Responses of Fungi Maggot (Bradysia impatiens Johannsen) to Allyl Isothiocyanate and High CO2

et al. 2022

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“…Two types of fluorescent silica nanoparticles (FL-SiO 2 NPs) with 70 nm and 150 nm sizes were prepared following the procedure reported by Bilal [ 32 ]. Cetyltrimethylammonium bromide was dissolved in deionized water under constant magnetic stirring with 1000 rpm, followed by the addition of sodium hydroxide (NaOH) solution.…”

Section: Methodsmentioning

confidence: 99%

“…Methoxyfenozide was loaded into FL-SiO 2 NPs according to the previously described procedures [ 32 , 33 ]. Normally, about 1.2 g of methoxyfenozide was dissolved in 40 mL of methanol (CH 3 OH) to obtain a methoxyfenozide solution with a 30 mg/mL concentration.…”

Section: Methodsmentioning

confidence: 99%

“…Methoxyfenozide loading content of the nanoparticles was measured by high-performance liquid chromatography (HPLC, 1200-DAD (Diode Array Detector), Agilent Santa Clara, CA, USA) using the method reported by Bilal et al (2020) [ 32 ]. Normally, loading content was determined through HPLC analysis after vigorous mixing of the 10 mg of Me@FL-SiO 2 NPs in 10 mL of acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

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Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

Bilal

Sial

Cao

et al. 2022

IJMS

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The diamond back moth, Plutella xylostella, causes severe damage at all crop stages, beside its rising resistance to all insecticides. The objective of this study was to look for a new control strategy such as application of insecticide-loaded carbon dot-embedded fluorescent mesoporous silica nanoparticles (FL-SiO2 NPs). Two different-sized methoxyfenozide-loaded nanoparticles (Me@FL-SiO2 NPs-70 nm, Me@FL-SiO2 NPs-150 nm) were prepared, with loading content 15% and 16%. Methoxyfenozide was released constantly from Me@FL-SiO2 NPs only at specific optimum pH 7.5. The release of methoxyfenozide from Me@FL-SiO2 NPs was not observed other than this optimum pH, and therefore, we checked and controlled a single release condition to look out for the different particle sizes of insecticide-loaded NPs. This pH-responsive release pattern can find potential application in sustainable plant protection. Moreover, the lethal concentration of the LC50 value was 24 mg/L for methoxyfenozide (TC), 14 mg/L for Me@FL-SiO2 NPs-70 nm, and 15 mg/L for Me@FL-SiO2 NPs-150 nm after 72 h exposure, respectively. After calculating the LC50, the results predicted that Me@FL-SiO2 NPs-70 nm and Me@FL-SiO2 NPs-150 nm exhibited better insecticidal activity against P. xylostella than methoxyfenozide under the same concentrations of active ingredient applied. Moreover, the activities of detoxification enzymes of P. xylostella were suppressed by treatment with insecticide-loaded NPs, which showed that NPs could also be involved in reduction of enzymes. Furthermore, the entering of FL-SiO2 NPs into the midgut of P. xylostella was confirmed by confocal laser scanning microscope (CLSM). For comparison, P. xylostella under treatment with water as control was also observed under CLSM. The control exhibited no fluorescent signal, while the larvae treated with FL-SiO2 NPs showed strong fluorescence under a laser excitation wavelength of 448 nm. The reduced enzyme activities as well as higher cuticular penetration in insects indicate that the nano-based delivery system of insecticide could be potentially applied in insecticide resistance management.

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“…The available literature on the entomotoxic effects of SiO2NPs focuses on insect pests of stored grains, such as Sitophilus oryzae (Goswami et al 2010;Debnath et al 2011), Callosobruchus maculatus (Rouhani et al 2012;Arumugam et al 2016), and Corcyra cephalonica (Vani and Brindhaa 2013). More recently, entomotoxic effects have also been tested on insect pests in field settings, including the lepidopterans Spodoptera littoralis (El-Samahy et al 2015;Ayoub et al 2017), Spodoptera litura, Achaea janata (Rani et al 2014), Mythimna separata (Mousa et al 2014) and Plutella xylostella (Shoaib et al 2018;Bilal et al 2020), as well as the aphids Lipaphis pseudobrassicae (Debnath et al 2010), Aphis gossypii (Pavitra et al 2018, and the mealybug Phenacoccus solenopsis (Pavitra et al 2018). These insects are external feeders and internal leaf feeders such as leafminers have not been well studied of SiO2NP effects.…”

Section: Introductionmentioning

confidence: 99%

Silica nanoparticles control a generalist pest, American serpentine leaf miner <em>Liriomyza trifolii</em>

Thabet¹,

Galal²,

Tuda³

et al. 2020

Preprint

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Insects quickly develop their resistance to conventional synthetic chemical insecticides. Silica nanoparticles (SiO2NPs) is a new promising approach not only in decreasing insect pest numbers but also for its safety regarding agricultural organisms such as plants and beneficial natural enemies. Here, we investigate the safety of SiO2NPs on the faba bean Vicia faba (Fabaceae) as a model plant for toxicity studies and its control efficiency against the American serpentine leafminer Liriomyza trifolii (Diptera: Agromyzidae) as a major devastating insect pest against a wide range of crops. The concentrations of 50, 100, 200 and 400 mg/L of SiO2NPs (spherical shape, 15.3 nm) as well as distilled water as the control were used to investigate the effect of the nanoparticles. Early stages of development of V. faba were evaluated. The same concentrations of SiO2NPs were applied by spraying on two weeks old bean seedlings to evaluate their toxic effect on the feeding, survival and body mass of L. trifolii. A qRT-PCR analysis was conducted to assess oxidative stress on the gene expression level of the major antioxidative enzymes, catalase and superoxide dismutase, in L. trifolii. SiO2NPs inhibited root development only at a high concentration and did not affect the germination percentage, germination time, shoot length and vigor index compared to the control. The survival rate of larvae was lower than the control at the highest concentration and the larval feeding velocity and pupal weight decreased at high concentrations. Gene expression of both enzymes at the pupal stage were not affected by SiO2NPs at any concentrations. This study suggests that care must be taken to utilize SiO2NPs at a suitable concentration in controlling pests, avoiding negative effects on plant growth.

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Indoxacarb‐loaded fluorescent mesoporous silica nanoparticles for effective control of Plutella xylostella L. with decreased detoxification enzymes activities

Cited by 42 publications

References 73 publications

Responses of Fungi Maggot (Bradysia impatiens Johannsen) to Allyl Isothiocyanate and High CO2

Responses of Fungi Maggot (Bradysia impatiens Johannsen) to Allyl Isothiocyanate and High CO2

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

Silica nanoparticles control a generalist pest, American serpentine leaf miner <em>Liriomyza trifolii</em>

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