Inducción de la degradación de lignina de paja de trigo en aromáticos por Aspergillus spp. y Penicillium chrysogenum

Ligninolytic activity of the Penicillium chrysogenum and Pleurotus ostreatus fungi involved in the biotransformation of synthetic multi-walled carbon nanotubes modify its toxicity

“…Relevantly, our findings indicate that both fungi species were capable to grow efficiently on medium supplemented with MWCNTs, demonstrating that this nanomaterial was no toxic at least to the concentration used (10 μg/mL of MWCNTs) and an efficient fungal growth and ligninolytic enzymes excretion was observed. The results confirm that lignin played a key role in the induction of ligninolytic enzymes such as those reported in basidiomycetes during the lignin depolymerization process ( Baltierra-Trejo et al, 2016 ; Cañas & Camarero, 2010 ; Nousiainen et al, 2014 ).…”

“…Assimilation of compounds such as lignin has been widely documented and correlated with the synthesis of enzymes such as LiP, MnP, or laccase ( Baltierra-Trejo et al, 2016 ; Chen et al, 2011 ; Ting et al, 2011 ). Thus, we determined the MWCNTs biodegradation capacity of P. chrysogenum and P. ostreatus fungi in cultures media supplemented with carbon nanomaterials, thereby inducing ligninolytic enzymes via the addition of lignin as a carbon source with or without glucose supplementation.…”

“…The initial inoculum was prepared by culturing each fungus in a potato dextrose agar medium (PDA; BD-Bioxon) for an incubation period of 7 d at 22 C. Subsequently, discs of the agar plate corresponding to approximately 1 cm 2 for each fungus were inoculated in a 100-mL liquid medium with the following composition (g/L): NH 4 NO 3 5.0, CuSO 4 0.01, MgSO 4 1.5, KH 2 PO 4 1.5, K 2 HPO 4 1.5, NaCl 0.9, yeast extract 1.0, bromothymol blue 10 ppm, and 1 mL/L of trace elements solution prepared as follows (g/L): H 3 BO 3 2.86, ZnSO 4 -7H 2 O 0.22, MnCl 2 -7H 2 O 1.81, and KMnO 4 0.09. The carbon source used corresponded to 0.5 g/L glucose and/or 2 g/L of residual lignin from semi-purified wheat straw based on Baltierra-Trejo et al (2016) and 10 mg/mL of MWCNTs at a pH of 5.5. The fungi cultures were incubated on a rotary shaker at 150 rpm for 28 d at 30 C.…”

Análisis Del Rendimiento Escolar Modalidad Virtual Y Presencial en La Unidad De Aprendizaje De Física Básica De La Universidad Autónoma De Campeche

“…El pH del medio de cultivo se ajustó a 5,5. Este se incubó durante 20 días a 28 ° C con agitación de 150 rpm a un pH de 5,5 ± 0,2, al final se filtró el extracto crudo de hongos (Baltierra et al, 2016) y se ajustó el pH a 6,5 ± 0,2 antes de añadirlo para la bioestimulación del suelo contaminado por el ARA. La humedad del suelo se ajustó al 80% de la capacidad de campo.…”

Conditioning and use of prickly pear peels for the production of lignocellulosic enzymes by Aspergillus niger sp. on solid-state cultures

“…produces hydrolytic enzymes, it can also produce oxidoreductases that degrade complex plant materials (Calle et al, 2007). Other wild strains of Aspergillus produce aromatic compounds, when cultivated in a liquid medium using residual lignin from wheat straw, with laccase titers close to 3 U g −1 of residual lignin for A. fumigatus after one week of culture and 2.4 U g −1 of residual lignin for A. tubigensis after three weeks (Baltierra-Trejo et al, 2016). Aromatic compounds or phenols are generally considered laccase inducers (Bertrand et al, 2013).…”