Induced Expression of Insulin-like Growth Factor-1 by Amniotic Membrane-Conditioned Medium in Cultured Human Corneal Epithelial Cells

Lee, Joon H; Ryu, Ik Hee; Kim, Eung Kweon; Lee, Jong Eun; Hong, Soon Won

doi:10.1167/iovs.05-0596

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…In the treatment of chronic corneal wounds, a relatively new approach that has been very successful in some of the most difficult to heal, persistent ulcers is the use of human amniotic membrane sheets that are sutured over the corneal defect 100 . The amniotic membrane is thought to act like an intact, functional basement membrane on which the corneal epithelial cells can migrate and attach, provide growth factors that are present in processed basement membrane, and act as a substrate for elevated proteases in tear film that reduces protease damage to the surface of the corneal ECM 100–102 . The success of this strategy in chronic corneal ulcers suggests that comparable treatments that provide both matrix and growth factors may be useful in other wound types.…”

Section: Implications For Difficult To Heal and Chronic Woundsmentioning

confidence: 99%

Interactions between extracellular matrix and growth factors in wound healing

Schultz

Wysocki

2009

Wound Repair Regeneration

942

725

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Dynamic interactions between growth factors and extracellular matrix (ECM) are integral to wound healing. These interactions take several forms that may be categorized as direct or indirect. The ECM can directly bind to and release certain growth factors (e.g., heparan sulfate binding to fibroblast growth factor-2), which may serve to sequester and protect growth factors from degradation, and/or enhance their activity. Indirect interactions include binding of cells to ECM via integrins, which enables cells to respond to growth factors (e.g., integrin binding is necessary for vascular endothelial growth factor-induced angiogenesis) and can induce growth factor expression (adherence of monocytes to ECM stimulates synthesis of platelet-derived growth factor). Additionally, matrikines, or subcomponents of ECM molecules, can bind to cell surface receptors in the cytokine, chemokine, or growth factor families and stimulate cellular activities (e.g., tenascin-C and laminin bind to epidermal growth factor receptors, which enhances fibroblast migration). Growth factors such as transforming growth factor-b also regulate the ECM by increasing the production of ECM components or enhancing synthesis of matrix degrading enzymes. Thus, the interactions between growth factors and ECM are bidirectional. This review explores these interactions, discusses how they are altered in difficult to heal or chronic wounds, and briefly considers treatment implications.

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Section: Implications For Difficult To Heal and Chronic Woundsmentioning

confidence: 99%

Interactions between extracellular matrix and growth factors in wound healing

Schultz

Wysocki

2009

Wound Repair Regeneration

942

725

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“…In ocular tissue, IGFBP3 has postulated roles in the human cornea in regulating myofibroblast proliferation and differentiation (Izumi et al, 2006); however, the localization and expression of IGFBP3 in the corneal epithelium has not been characterized. By contrast, IGF-1R has been localized to human corneal and conjunctival cells and IGF-1 has been shown to play an important role in corneal epithelial wound healing, stimulating both proliferation of corneal epithelial cells and migration (Lee et al, 2006;Rocha et al, 2001). Clinically, the topical administration of IGF-1 in conjunction with Substance P has been shown to stimulate epithelial repair in patients with persistent corneal epithelial defects (Nagama et al, 2003;Nishida, 2005).…”

Section: Introductionmentioning

confidence: 99%

Insulin-like Growth Factor Binding Protein-3 expression in the human corneal epithelium

Robertson

Hansen

et al. 2007

Experimental Eye Research

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Insulin-like Growth Factor Binding Protein-3 (IGFBP3) is a high affinity binding protein shown to regulate cell growth, differentiation, and apoptosis in a variety of cellular systems. The primary aim of this study was to characterize IGFBP3 expression in the human corneal epithelium and in a corneal epithelial cell line and to establish a potential role for IGFBP3 mediated apoptotic signaling in corneal epithelial cells.Using a telomerase-immortalized human corneal epithelial (hTCEpi) cell line cultured in serum-free media and fresh human eye bank donor tissue, expression and localization of IGFBP3 were established in situ and in vitro by indirect immunofluorescence and western blotting. Real time PCR was used to measure IGFBP3 mRNA levels following Trichostatin-A (TSA) treatment and as a function of confluence. IGFBP3 protein levels were assessed in resting human tears and in conditioned media by western blotting; as was the ability of recombinant human IGFBP3 protein to associate with the cell surface. Apoptotic signaling was assessed in vitro using TSA and recombinant human (rh)IGFBP3. Apoptosis was measured by Viability/Cytotoxicity, Annexin V, and TUNEL assays.IGFBP3 localized to the plasma membrane of human corneal epithelial cells in situ and was upregulated in surface cells in the central cornea. IGFBP3 was secreted in conditioned media of growing cells, with a robust upregulation following confluence (P<0.001) and differentiation. IGFBP3 was undetectable in human tears. Addition of TSA to the culture media resulted in an upregulation of IGFBP3 mRNA (P<0.001) and protein. In addition, TSA treatment led to a significant increase in Annexin V positive cells at 18 and 24 hours (P<0.001) and TUNEL positive cells at 24 and 48 hours (P<0.001). The addition of rhIGFBP3 to the cell culture media appeared to induce occasional membrane blebbing, but cells failed to become positive with Annexin V or TUNEL.Taken together, these results demonstrate that cell membrane-associated IGFBP3 is produced by corneal epithelial cells and associates with the plasma membrane of superficial cells in situ and in cultured cells, but is not present in human tears. The differential localization and effect(s) on apoptosis suggest that the effects of IGFBP3 are likely tissue compartment and receptor specific and may be regulated by glycosylation.

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“…Lee et al and Bischoff et al have identified the presence of GFs/cytokines in the CM obtained from the AM. They found IGF (61) , EGF, bFGF, IL-6, and IL-8 (62) in AM-CM and observed an increased proliferation of human corneal epithelial cells when treated with such a CM. Kim et al demonstrated that the use of CM from human AM epithelial cells exerts positive effects on wound healing in an experimental rabbit model with a corneal alkaline injury (63) .…”

Section: Therapeutic Use Of Gf/cytokines In Ocular Surface Diseasesmentioning

confidence: 99%

Biological modulation of corneal epithelial wound healing

Loureiro

Gomes

2019

Arquivos Brasileiros De Oftalmologia

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The transparency and maintenance of corneal epithelial integrity are essential for its optical properties and, to preserve these characteristics, the epithelium undergoes continuous renewal. This renewal depends on the control of cell proliferation and differentiation mediated by mitogenic factors responsible for increasing mitoses and stimulating cellular migration. Cell-cell communication plays a pivotal role in epithelial healing process, and several cytokines and growth factors are involved in this process. Understanding the cross-talk and paracrine effects of these cytokines and growth factors released can help in the search for new therapeutic strategies to treat ocular surface diseases.

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Induced Expression of Insulin-like Growth Factor-1 by Amniotic Membrane-Conditioned Medium in Cultured Human Corneal Epithelial Cells

Abstract: IGF-1 is induced by AMCM during HCEC proliferation, and this induction may play an important role in the amniotic membrane during HCEC proliferation and migration in several intractable corneal epithelial defects.

Cited by 12 publications

References 25 publications

Interactions between extracellular matrix and growth factors in wound healing

Interactions between extracellular matrix and growth factors in wound healing

Insulin-like Growth Factor Binding Protein-3 expression in the human corneal epithelium

Biological modulation of corneal epithelial wound healing

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