1976
DOI: 10.1093/nar/3.1.261
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Induced formation of covalent bonds between nucleoprotein components{middle dot} V{middle dot} UV or bisulfite induced polynucleotide-protein crosslinkage in bacteriophage MS2

Abstract: UV (lambda = 254 nm) irradiation of bacteriophage MS2 or its treatment with bisulfite induce covalent crosslinkage of the RNA to the coat protein. epilsonN-(2-oxopyrimidyl-4)-lysine was found in the phage hydrolysates after either type of treatment. An equimolar mixture of 0-methylhydroxylamine and bisulfite causes complete disappearance of the cross-links. This led to the conclusion that one of the factors responsible for the UV-induced polynucleotide-protein crosslinkage and the main factor in treatment with… Show more

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Cited by 21 publications
(13 citation statements)
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“…The field of promotion of nucleic acid-protein adduct formation by nucleophilic catalysis has been reviewed by Shapiro (1976). More recently Budowsky et al (1976) have given details on the cross-linking of proteins to nucleic acids in the RNA phage MS-2 via bisulfite and cytosine hydrate mediated processes. The photochemical aspects of this work were discussed in Section 2.3.…”
Section: Cross-linking By Means Of Transamination Reactionsmentioning
confidence: 99%
“…The field of promotion of nucleic acid-protein adduct formation by nucleophilic catalysis has been reviewed by Shapiro (1976). More recently Budowsky et al (1976) have given details on the cross-linking of proteins to nucleic acids in the RNA phage MS-2 via bisulfite and cytosine hydrate mediated processes. The photochemical aspects of this work were discussed in Section 2.3.…”
Section: Cross-linking By Means Of Transamination Reactionsmentioning
confidence: 99%
“…Budowsky and co‐workers have indicated that cytosine hydrates, formed by UVC irradiation of the RNA bacteriophage MS2, are responsible for the observed cross‐linking of the coat protein to the genomic RNA of this virus. These hydrates evidently react thermally with lysines in the coat protein of this phage, resulting in RNA‐protein cross‐linking.…”
Section: Discussionmentioning
confidence: 99%
“…Electronic excitation increases the reactivity of bases converting them into strong nucleophilic, electrophilic or free-radical reagents [20] which can efficiently attack, due to the rigid quaternary structure of nucleoprotein, only aminoacid residues in close proximity [7,8]. Some of these reactions may result in the formation of covalent bonds between polynucleotide and protein, e.g.…”
Section: Discussionmentioning
confidence: 99%
“…This method is adequate for fixation of polynucleotideprotein contacts in intact nucleoproteins [7,8] including ribosomes [9-121. 30-S subunits were reconstituted from a mixture of core subparticles and split proteins, containing in each case a single "'Ilabelled protein component.…”
mentioning
confidence: 99%