Induced mutation in ELONGATED HYPOCOTYL5 abolishes anthocyanin accumulation in the hypocotyl of pepper

Chen, Rong; Chao, Yang; Hu, Gao; Shi, Chunmei; Zhang, Zhiying; Lu, Guangyu; Shen, Xinyan; Tang, Yaping; Li, Feng; Lu, Yongen; Ouyang, Bo

doi:10.1007/s00122-022-04192-y

Cited by 11 publications

(6 citation statements)

References 63 publications

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“…HY5 acts downstream of the photoreceptor phytochrome B, and plays critical roles in plant growth, stress response, and several hormonal pathways in a light-dependent manner [ 12 , 13 , 24 ]. Therefore, the CaHY5 [ 25 ] protein was assumed to be an important candidate to further investigate the mechanism of action of red light in regulating pepper resistance to PCI. An alignment of deduced amino acid sequences of CaHY5 and its homolog in Arabidopsis (HY5) indicated that pepper CaHY5 has a high sequence similarity to AtHY5 ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Red light induces salicylic acid accumulation by activating CaHY5 to enhance pepper resistance against Phytophthora capsici

Yang,

Li,

Guang

et al. 2023

Horticulture Research

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Pepper (Capsicum annuum L.) is frequently challenged by various pathogens, among which Phytophthora capsici is the most devastating to pepper production. Red light signal acts as a positive induction of plant resistance against multiple pathogens. However, little is known about how the red light signal affects pepper resistance to P. capsici infection (PCI). Here, we report that red light regulates SA accumulation by activating CaHY5, a basic leucine zipper (bZIP) transcription factor, thereby decreasing pepper susceptibility to PCI. Exogenous SA treatment reduced pepper susceptibility to PCI, while silencing of CaPHYB (a red light photoreceptor) increased its susceptibility. PCI significantly induced CaHY5 expression, and silencing of CaHY5 reduced SA accumulation, accompanied by decreases in the expression levels of CaPAL3, CaPAL7, CaPR1, and CaPR1L, which finally resulted in higher susceptibility of pepper to PCI. Moreover, CaHY5 was found to activate the expression of CaPAL3 and CaPAL7, which are essential for SA biosynthesis, by directly binding to their promoters. Further analysis revealed that exogenous SA treatment could restore the resistance of CaHY5silenced pepper plants to PCI. Collectively, this study reveals a critical mechanism through which red light induces SA accumulation by regulating CaHY5mediated CaPAL3 and CaPAL7 expression, leading to enhanced resistance to PCI. Moreover, red light-induced CaHY5 regulates pepper resistance to PCI, which may have implications for PCI control in protected vegetable production.

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Section: Resultsmentioning

confidence: 99%

Red light induces salicylic acid accumulation by activating CaHY5 to enhance pepper resistance against Phytophthora capsici

Yang,

Li,

Guang

et al. 2023

Horticulture Research

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“…The MYBA gene locus, which in uences the accumulation of anthocyanins in various pepper tissues, has been genetically mapped to chromosome 10 ( Borovsky et al 2004). Further investigations have substantiated the speci c genes associated with purple fruit and purple hypocotyls in pepper (Chen et al 2022;Byun et al 2022). Cotyledon color can be utilized as a more convenient and e cient morphological marker during the seedling stage in the exploitation of pepper heterosis, compared to molecular markerassisted breeding.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, researchers identi ed a crucial gene HY5 which mutated causing the production of a pepper green hypocotyl mutant. CaHY5 regulated the accumulation of anthocyanin in pepper hypocotyls by binding to the promoters of downstream genes partcipated in anthocyanin biosynthesis and modi cation (Chen et al 2022). Leaf color can also serve as a morphological marker during the seedling stage.…”

Section: Introductionmentioning

confidence: 99%

Fine mapping of CaMYB113 regulating anthocyanin accumulation in pepper cotyledons

liu,

Yang,

Zhang

et al. 2023

Preprint

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Cotyledon color can serve as a convenient and efficient morphological marker in F1 hybrid seeds screening, however, few studies on mutants affecting the color of cotyledon have been reported. A genetic population was generated by crossing 21C1344 with purple cotyledon and 21C912 with green cotyledon and the localization of CaMYB113 on chromosome 10 was determined. The results of virus-induced gene silencing (VIGS) demonstrated a substantial decrease in anthocyanin accumulation in cotyledons upon silencing of CaMYB113. The overexpression of the CaMYB113 gene not only resulted in purple pigmentation in both callus and leaves, but also changed the expression levels of downstream genes involved in anthocyanin synthesis. CaMYB113 could bind to crucial genes related to anthocyanin biosynthesis demonstrated by the yeast one-hybrid assay, thereby regulating anthocyanin production and accumulation in capsicum cotyledons. The gene we identified could be converted into a reliable selection marker for achieving stable genetic transformation, while also providing valuable insights for maintaining purity in pepper hybrid seeds.

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“…The HY5 protein directly binds to either G-box or ACE-box in the promoters of anthocyanin biosynthetic genes such as CHS and DFR, then activates their expression, positively regulating anthocyanin accumulation [30]. CaHY5 can bind to the promoter of CaF3H, CaF3 5 H, CaDFR, CaANS and CaGST, which are well related to anthocyanin biosynthesis or transport, and thereby promote anthocyanin accumulation in pepper hypocotyl [31]. However, residual anthocyanins have still been present in hy5 mutants of Arabidopsis, which indicates that there is an anthocyanin induction pathway that is independent of HY5 in plants [30,32].…”

Section: Introductionmentioning

confidence: 99%

Omics Analysis Unveils the Pathway Involved in the Anthocyanin Biosynthesis in Tomato Seedling and Fruits

Liu

Zhang

et al. 2023

IJMS

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The purple tomato variety ‘Indigo Rose’ (InR) is favored due to its bright appearance, abundant anthocyanins and outstanding antioxidant capacity. SlHY5 is associated with anthocyanin biosynthesis in ‘Indigo Rose’ plants. However, residual anthocyanins still present in Slhy5 seedlings and fruit peel indicated there was an anthocyanin induction pathway that is independent of HY5 in plants. The molecular mechanism of anthocyanins formation in ‘Indigo Rose’ and Slhy5 mutants is unclear. In this study, we performed omics analysis to clarify the regulatory network underlying anthocyanin biosynthesis in seedling and fruit peel of ‘Indigo Rose’ and Slhy5 mutant. Results showed that the total amount of anthocyanins in both seedling and fruit of InR was significantly higher than those in the Slhy5 mutant, and most genes associated with anthocyanin biosynthesis exhibited higher expression levels in InR, suggesting that SlHY5 play pivotal roles in flavonoid biosynthesis both in tomato seedlings and fruit. Yeast two-hybrid (Y2H) results revealed that SlBBX24 physically interacts with SlAN2-like and SlAN2, while SlWRKY44 could interact with SlAN11 protein. Unexpectedly, both SlPIF1 and SlPIF3 were found to interact with SlBBX24, SlAN1 and SlJAF13 by yeast two-hybrid assay. Suppression of SlBBX24 by virus-induced gene silencing (VIGS) retarded the purple coloration of the fruit peel, indicating an important role of SlBBX24 in the regulation of anthocyanin accumulation. These results deepen the understanding of purple color formation in tomato seedlings and fruits in an HY5-dependent or independent manner via excavating the genes involved in anthocyanin biosynthesis based on omics analysis.

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Induced mutation in ELONGATED HYPOCOTYL5 abolishes anthocyanin accumulation in the hypocotyl of pepper

Cited by 11 publications

References 63 publications

Red light induces salicylic acid accumulation by activating CaHY5 to enhance pepper resistance against Phytophthora capsici

Red light induces salicylic acid accumulation by activating CaHY5 to enhance pepper resistance against Phytophthora capsici

Fine mapping of CaMYB113 regulating anthocyanin accumulation in pepper cotyledons

Omics Analysis Unveils the Pathway Involved in the Anthocyanin Biosynthesis in Tomato Seedling and Fruits

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