Induced myelomonocytic differentiation in leukemia cells is accompanied by noncanonical transcription factor expression

Abstract

HighlightsWe surveyed common myeloid leukemia cell lines undergoing differentiation therapy.Lineage progression in induced leukemia cell lines differs from nonmalignant cells.Aberrant transcription factor expression occurs in acute myeloid leukemia cells.A given differentiation therapy elicits different effects in specific cell lines.Although potent tools, cell lines must be employed with circumspection.

An Effective Model of the Retinoic Acid Induced HL-60 Differentiation Program

“…We assembled the connectivity of the signal integration and phenotypic programs driven by Trigger and cRaf-pS621 from literature (Table 1 ). We estimated the parameters for the signal initiation, and phenotype modules from steady-state and dynamic measurements of transcription factor and phenotypic marker expression following the addition of ATRA 28 – 31 . However, the bulk of the model parameters were taken from literature 32 and were not estimated in this study (see materials and methods).…”

Jak-Stat Signaling Pathway Related Gene Expressions and Blood Biochemical indicators in Acute Promyelocytic Leukemia

“…Một nghiên cứu gần đây đã chỉ ra rằng, biểu hiện gen KL giảm ảnh hưởng đến sự tăng nồng độ của 1,25-Dihydroxyvitamin D3 (D3) trong máu, dẫn đến hiện tượng các khớp xương bị vôi hóa sớm, bệnh lão hóa tim mạch và hệ miễn dịch bất hoạt [3]. Tế bào ung thư APL khi tiếp xúc với D3 có biểu hiện hoạt hóa tăng lên dẫn đến sự tăng sinh nhiều hơn so với tế bào không được xử lý [24]. Chính vì vậy, biểu hiện giảm gen KL liên quan một phần đến sự tăng sinh của tế bào APL Trong nghiên cứu này, chúng tôi đã chỉ ra rằng bệnh nhân APL có biểu hiện các gen STAT tăng rõ rệt so với người khỏe mạnh và kết quả này có ý nghĩa thống kê, trong khi biểu hiện gen IκB-α không thay đổi trong tế bào APL.…”

An Effective Model of the Retinoic Acid Induced HL-60 Differentiation Program

“…Gene expression was normalized to expression in the absence of ATRA. Experimental data in panels A and B were reproduced from Jensen et al 31 . Model simulations were conducted using the ten best parameter sets collected during model identification.…”

“…While the makeup of the assembled the connectivity of the signal integration and phenotypic programs driven by Trigger and cRaf-pS621 from literature (Table 1). We estimated the parameters for the signal initiation, and phenotype modules from steady-state and dynamic measurements of transcription factor and phenotypic marker expression following the addition of ATRA [28][29][30][31] . However, the bulk of the model parameters were taken from literature 32 and were not estimated in this study (see materials and methods).…”

“…Solid lines (or bars) denote the mean model performance, while the shaded region (or error bars) denotes the 95% confidence estimate of the polynomial model. Experimental data in panel B was reproduced from Jensen et al31 .…”