Induced of plastid mutations in soybean plant (Glycine max L. Merrill) with gamma radiation and determination with RAPD

Atak, Çimen; Alikamanoğlu, Sema; Açık, Leyla; Canbolat, Yasemin

doi:10.1016/j.mrfmmm.2004.06.037

Cited by 47 publications

(30 citation statements)

References 14 publications

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“…For radiation-induced mutation JCL accessions, only JCL25 and JCL26 were separated in another group, whereas irradiated JCL27, JCL28 and JCL29 showed no polymorphism at nucleotide or DNA methylation and were clustered along with JCL in the first group. Irradiation by X-rays and γ-rays can induce nucleotide changes in many plants such as mungbean (Sangsiri et al, 2005) and soybean (Atak et al, 2004) and these changes could be detected using DNA markers (Danylchenko and Sorochinsky, 2005). The results of the present study indicated that mutation induced by irradiation caused some changes in DNA sequences in samples JCL25 and JCL26 as well as DNA methylation alterations, but possibly not in irradiated JCL27, JCL28 and JCL29, which were induced mutations at low levels of radiation (300 rad).…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism

Kanchanaketu

Sangduen²,

Toojinda³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Genetic analysis of 56 samples of Jatropha curcas L. collected from Thailand and other countries was performed using the methylation-sensitive amplification polymorphism (MSAP) technique. Nine primer combinations were used to generate MSAP fingerprints. When the data were interpreted as amplified fragment length polymorphism (AFLP) markers, 471 markers were scored. All 56 samples were classified into three major groups: γ-irradiated, non-toxic and toxic accessions. Genetic similarity among the samples was extremely high, ranging from 0.95 to 1.00, which indicated very low genetic diversity in this species. The MSAP fingerprint was further analyzed for DNA methylation polymorphisms. The results revealed differences in the DNA methylation level among the samples. However, the samples collected from saline Genetic diversity analysis of Jatropha curcas areas and some species hybrids showed specific DNA methylation patterns. AFLP data were used, together with methylation-sensitive AFLP (MS-AFLP) data, to construct a phylogenetic tree, resulting in higher efficiency to distinguish the samples. This combined analysis separated samples previously grouped in the AFLP analysis. This analysis also distinguished some hybrids. Principal component analysis was also performed; the results confirmed the separation in the phylogenetic tree. Some polymorphic bands, involving both nucleotide and DNA methylation polymorphism, that differed between toxic and non-toxic samples were identified, cloned and sequenced. BLAST analysis of these fragments revealed differences in DNA methylation in some known genes and nucleotide polymorphism in chloroplast DNA. We conclude that MSAP is a powerful technique for the study of genetic diversity for organisms that have a narrow genetic base.

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Section: Resultsmentioning

confidence: 99%

Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism

Kanchanaketu

Sangduen²,

Toojinda³

et al. 2012

Genet. Mol. Res.

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“…RAPD is an efficient method to detect DNA alteration via using random primers. It has been started to use in earlier studies of genetic variabilities obtained by radiation treatments in Chrysanthemum [36,37], soybean [24], sugarcane, sunflower, groundnut [43], tobacco [23], potato [27], Rhododendron [25]. ISSR method is another molecular marker method widely used in plant biotechnology applications.…”

Section: Mutational Genomic Analysismentioning

confidence: 99%

“…Atak et al [24] used 100-500 Gy radiation doses produced by 137 Cs gamma source for soybean seeds, while Singh and Datta [29] used 60 Co gamma source at different doses ranging between 10 and 100 Gy for Triticum aestivum seeds. Ulukapı et al [21] also used 60 Co gamma source at 80-240 Gy radiation doses to induce genetic variability for Solanum melongena L. Çelik and Atak [23] used 100, 200, 300, and 400 Gy gamma rays by 137 Cs to determine the effective radiation dose for breeding studies of two Turkish tobacco varieties.…”

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confidence: 99%

Applications of Ionizing Radiation in Mutation Breeding

Çelik¹,

Atak²

2017

New Insights on Gamma Rays

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As a predicted result of increasing population worldwide, improvements in the breeding strategies in agriculture are valued as mandatory. The natural resources are limited, and due to the natural disasters like sudden and severe abiotic stress factors, excessive floods, etc., the production capacities are changed per year. In contrast, the yield potential should be significantly increased to cope with this problem. Despite rich genetic diversity, manipulation of the cultivars through alternative techniques such as mutation breeding becomes important. Radiation is proven as an effective method as a unique method to increase the genetic variability of the species. Gamma radiation is the most preferred physical mutagen by plant breeders. Several mutant varieties have been successfully introduced into commercial production by this method. Combinational use of in vitro tissue culture and mutation breeding methods makes a significant contribution to improve new crops. Large populations and the target mutations can be easily screened and identified by new methods. Marker assisted selection and advanced techniques such as microarray, next generation sequencing methods to detect a specific mutant in a large population will help to the plant breeders to use ionizing radiation efficiently in breeding programs.

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“…Induced mutation contributes to genetic variability by increasing DNA polymorphism. Mutagenesis disturbs the normal biological composition, and there are 2 effects of ionizing radiation on the heredity material: gene mutations and chromosome breaks (Atak et al, 2004;Dhakshanamoorthy et al, 2011). Generally, the presence or absence of RAPD bands is used to estimate the diversity of the samples.…”

Section: Evaluation Of Genetic Variation With Rapd Markersmentioning

confidence: 99%

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

Yunus¹,

Aziz²,

Kadir³

et al. 2013

Turk J Biol

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Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of irradiation on in vitro buds of E. elatior showed LD 50 to be 10 Gy, with the survival of explants being sharply reduced at this dosage. All 8 selected gamma irradiated regenerants were differentiated from the untreated control based on the banding patterns obtained using 9 primers, which generated 59 reproducible bands, whereby 35 (55.31%) were found to be polymorphic. Jaccard's coefficient of similarity values ranging from 0.537 to 0.860 were indicative of the level of genetic variation among the mutants studied. For comparison between the potential lines (PL) and the control, a maximum similarity value (0.814) was observed in PL1 mutant, while the minimum value (0.537) was observed in PL7. In summary, a combination of irradiation, regeneration, multiplication, and random amplification of polymorphic DNA (RAPD) analysis for early screening of mutants can speed up the breeding program of E. elatior.

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Induced of plastid mutations in soybean plant (Glycine max L. Merrill) with gamma radiation and determination with RAPD

Cited by 47 publications

References 14 publications

Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism

Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism

Applications of Ionizing Radiation in Mutation Breeding

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

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