Induced oxidative stress management in wounds through phenolic acids engineered fibrous protein: An in vitro assessment using polymorphonuclear (PMN) cells

Catechol-functionalized hydrogels: biomimetic design, adhesion mechanism, and biomedical applications

“…28,29 The challenges of developing tough and robust underwater bonding are analogous to the adhesion problems solved by marine mussels, which are naturally equipped with reliable strategies to achieve interfacial adhesion in dynamic and turbulent environments. 27,[30][31][32] 3,4dihydroxy-L-phenyalanine (DOPA), a catecholic amino acid present in mussel adhesive proteins, has been identified as being able to penetrate water boundary layers, and subsequently interacts with local metal ions to form adhesive bonds with substrates. [33][34][35] As a result, the catechol moieties of DOPA have been exploited as a popular inspiration for the design of biomimetic wet adhesives.…”

Gap closure of different shape wounds: in vitro and in vivo experimental models in the presence of engineered protein adhesive hydrogel

“…Between the experimental groups, significant reduction ( P < 0.05) was observed between control and gallic acid alone, and the reduction was significant at P < 0.01 between control and engineered protein. In our previous research studies, we proved that the addition of GEG to fibroblast cells enhances the in vitro cell migration in the scratch wound model (Thiruselvi et al, ). Also, our assessment with the polymorphonuclear cells suggested that the engineered protein was involved in the management of oxidative stress (Thiruselvi et al, ).…”

“…In our previous research studies, we proved that the addition of GEG to fibroblast cells enhances the in vitro cell migration in the scratch wound model (Thiruselvi et al, ). Also, our assessment with the polymorphonuclear cells suggested that the engineered protein was involved in the management of oxidative stress (Thiruselvi et al, ). Further, addition of antioxidants (reduced glutathione) to the fibroblast cells influences the contraction of gel area (Deveci et al, ).…”

“…In our earlier study, we employed an engineered protein in the form of gallic acid engineered gelatin (GEG) as an effective wound healing agent (Thiruselvi et al, , ), which acts as an extracellular matrix to promote cell proliferation and migration. In addition, the antioxidant profile in controlling the cellular reactive oxygen species (ROS) of GEG is also studied in detail.…”

“…Bioadhesive hydrogel was prepared according to the procedure summarized (Thiruselvi et al, ), which consists of two steps, namely, Step 1, involving the preparation of GEG, and Step 2, involving the transformation of GEG to hydrogel, which is designated as [GEG]O.…”