Induced pluripotent stem cell‐conditional medium inhibits H9C2 cardiomyocytes apoptosis via autophagy flux and Wnt/β‐catenin pathway

Guo, Xinhong; Gu, Xiaohong; Sohun, Hareshwaree; Rong, Xing; Li, Lei; Chu, Maoping

doi:10.1111/jcmm.14327

Cited by 15 publications

(12 citation statements)

References 62 publications

(71 reference statements)

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“…We measured the Wnt11 in CdM Wnt11 with semi-quantitative Western blotting. The concentration of Wnt11 in CdM Wnt11 was significantly higher than that in CdM GFP , which is consistent with previous reports that Wnt can be secreted from stem cells [45,46]. Therefore, the cardiac injury following ischemia may be, at least partially, due to the loss of non-canonical Wnt signaling.…”

Section: Discussionsupporting

confidence: 90%

WNT11-Conditioned Medium Promotes Angiogenesis through the Activation of Non-Canonical WNT-PKC-JNK Signaling Pathway

Wang

Gong

Zuo

et al. 2020

Genes

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Background: We demonstrated that the transduction of Wnt11 into mesenchymal stem cells (MSCs) (MSCWnt11) promotes these cells differentiation into cardiac phenotypes. In the present study, we investigated the paracrine effects of MSCWnt11 on cardiac function and angiogenesis. Methods and Results: Conditioned medium was collected from MSCWnt11 (CdMWnt11) and their control cells (CdMGFP). CdMWnt11, especially obtained from MSCWnt11 exposed to hypoxia, significantly promoted human umbilical vein endothelial cells (HUVECs) migration and increased capillary-like tube (CLT) formation, which was blocked by Wnt11 neutralizing antibody. Wnt11 protein was significantly higher in CdMWnt11 compared to that in CdMGFP. Directly treating HUVECs with recombinant Wnt11 protein significantly increased CLT formation, which was abrogated by treating cells with the JNK inhibitor SP600125, as well as the PKC inhibitor Calphostin-C. Moreover, the transfection of Wnt11 to HUVECs (HWnt11) significantly increased CLT formation and HUVEC migration, as well as upregulated p-pan-PKC and p-JNK expression. Injection of CdMWnt11 into the peri-infarct region in a rat acute myocardial infarction (AMI) model significantly improved cardiac function, reduced infarct size, and increased myocardial blood flow and blood vessel density in the ischemic area. Conclusion: Wnt11 released from MSCWnt11 increased angiogenesis and improved cardiac function via non-canonical Wnt-PKC-JNK dependent pathways.

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Section: Discussionsupporting

confidence: 90%

WNT11-Conditioned Medium Promotes Angiogenesis through the Activation of Non-Canonical WNT-PKC-JNK Signaling Pathway

Wang

Gong

Zuo

et al. 2020

Genes

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“…Moreover, Leroux et al (48) reported that MScs, via the Wnt4 pathway, improved skeletal muscle fiber regeneration following ischemic injury. Other studies have also demonstrated activation of the Wnt pathway by MScs (55)(56)(57). The present study found that, in the presence of hdPSc-cM, the levels of Wnt/β-catenin pathway-related proteins, including p-GSK-3β and β-catenin, were upregulated.…”

Section: Discussionsupporting

confidence: 72%

The secretome of human dental pulp stem cells protects myoblasts from hypoxia‑induced injury via the Wnt/β‑catenin pathway

Zhang

Han

et al. 2020

Int J Mol Med

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Human dental pulp stem cells (hdPScs) present several advantages, including their ability to be non-invasively harvested without ethical concern. The secretome of hdPScs can promote the functional recovery of various tissue injuries. However, the protective effects on hypoxia-induced skeletal muscle injury remain to be explored. The present study demonstrated that c2c12 myoblast coculture with hdPScs attenuated cocl 2-induced hypoxic injury compared with c2c12 alone. The hdPSc secretome increased cell viability and differentiation and decreased G2/M cell cycle arrest under hypoxic conditions. These results were further verified using hDPSC-conditioned medium (hDPSC-CM). The present data revealed that the protective effects of hdPSc-cM depend on the concentration ratio of the cM. In terms of the underlying molecular mechanism, hdPSc-cM activated the Wnt/β-catenin pathway, which increased the protein levels of Wnt1, phosphorylated-glycogen synthase kinase-3β and β-catenin and the mRNA levels of Wnt target genes. By contrast, an inhibitor (XAV939) of Wnt/β-catenin diminished the protective effects of hdPSc-cM. Taken together, the findings of the present study demonstrated that the hDPSC secretome alleviated the hypoxia-induced myoblast injury potentially through regulating the Wnt/β-catenin pathway. These findings may provide new insight into a therapeutic alternative using the hdPSc secretome in skeletal muscle hypoxia-related diseases.

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“…Substituted 3‐formyl‐2‐quinoline and 3‐formyl‐2‐quinolones, used in our study as starting materials, have been reported for their antimicrobial and antifungal activities. [ 54 ] The literature reports reveal that the quinoline derivatives displayed good antibacterial activity against both Gram‐negative and Gram‐positive bacterial strains [ 55 ] and have immense potential to control methicillin‐resistant Staphylococcus aureus infection. [ 56 ]…”

Section: Resultsmentioning

confidence: 99%

Potential antibacterial and antifungal activities of novel sulfamidophosphonate derivatives bearing the quinoline or quinolone moiety

Bazine

Bendjedid²,

Boukhari

2020

Archiv der Pharmazie

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A series of new α‐sulfamidophosphonate/sulfonamidophosphonate ( 4a – n ) and cyclosulfamidophosphonate ( 5a – d ) derivatives containing the quinoline or quinolone moiety was designed and synthesized via Kabachnik–Fields reaction in the presence of ionic liquid under ultrasound irradiation. This efficient methodology provides new 1,2,5‐thiadiazolidine‐1,1‐dioxide derivatives 5a – d in one step and optimal conditions. The molecular structures of the novel compounds 4a – n and 5a – d were confirmed using various spectroscopic methods. All these compounds were evaluated for their in vitro antibacterial activity against Gram‐negative ( Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853) and Gram‐positive ( Staphylococcus aureus ATCC 27923) bacteria, in addition to three clinical strains ( E. coli 1, P. aeruginosa 1, and S. aureus 1). Most of the tested compounds showed more potent inhibitory activities against both Gram‐positive and ‐negative bacteria compared with the sulfamethoxazole reference. The following compounds, 4n , 4f , 4g , 4m , 4l , 4d , and 4e , are the most active sulfamidophosphonate derivatives. Furthermore, these molecules gave interesting zones of inhibition varying between 28 and 49 mm, against all tested bacterial strains, with a low minimum inhibitory concentration (MIC) value ranging from 0.125 to 8 μg/ml. All the synthesized derivatives were also evaluated for their in vitro antifungal activity against Fusarium oxyporum f. sp. lycopersici and Alternaria sp. The results revealed that all the synthesized compounds exhibited excellent antifungal inhibition and the compounds 4f , 4g , 4m , and 4i were the most potent derivatives with MIC values ranging from 0.25 to 1 µg/ml against the two tested fungal strains. The strongest inhibition of bacteria and fungi strains was detected by the effect of quinolone and sulfamide moieties.

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Induced pluripotent stem cell‐conditional medium inhibits H9C2 cardiomyocytes apoptosis via autophagy flux and Wnt/β‐catenin pathway

Cited by 15 publications

References 62 publications

WNT11-Conditioned Medium Promotes Angiogenesis through the Activation of Non-Canonical WNT-PKC-JNK Signaling Pathway

WNT11-Conditioned Medium Promotes Angiogenesis through the Activation of Non-Canonical WNT-PKC-JNK Signaling Pathway

The secretome of human dental pulp stem cells protects myoblasts from hypoxia‑induced injury via the Wnt/β‑catenin pathway

Potential antibacterial and antifungal activities of novel sulfamidophosphonate derivatives bearing the quinoline or quinolone moiety

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