Induced pluripotent stem cell-derived vascular smooth muscle cells: methods and application

Dash, Biraja C.; Jiang, Zhengxin; Suh, Carol Y.; Qyang, Yibing

doi:10.1042/bj20141078

Cited by 54 publications

(42 citation statements)

References 82 publications

(108 reference statements)

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“…The WBS-hiPSC-SMC phenotype was rescued with the use of rapamycin, which inhibits smooth muscle proliferation through the mammalian target of rapamycin (mTOR) pathway (80). This result supports the testing of rapamycin in treatment of individuals with WBS and represents an example of the use of hiPSCs for modeling of diseases associated with smooth muscle proliferation and for the discovery of treatment modalities to be tried in the clinic (81). In attempts to model disease in a dish, hiPSC-SMCs were derived from patients with supravalvular aortic stenosis (82); hiPSC-differentiated cardiomyocytes were derived from patients with the LEOPARD syndrome (83); and hiPSC-ECs were derived from patients with the moyamoya disease (idiopathic cerebrovascular disease) (84).…”

Section: Models and Mechanismssupporting

confidence: 72%

Vascular diseases await translation of blood vessels engineered from stem cells

2015

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The discovery of human induced pluripotent stem cells (hiPSCs) might pave the way toward a long-sought solution for obtaining sufficient numbers of autologous cells for tissue engineering. Several methods exist for generating endothelial cells or perivascular cells from hiPSCs in vitro for use in the building of vascular tissue. We discuss current developments in the generation of vascular progenitor cells from hiPSCs and the assessment of their functional capacity in vivo, opportunities and challenges for the clinical translation of engineered vascular tissue, and modeling of vascular diseases using hiPSC-derived vascular progenitor cells.

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Section: Models and Mechanismssupporting

confidence: 72%

Vascular diseases await translation of blood vessels engineered from stem cells

2015

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“…It is known that resident vascular stem cells participate in the repair of vessel damages using its ability to undergo differentiation into cell types such as endothelial cells and SMCs . Moreover, the development or repair of vascular damages is affected by whether the stem cells differentiate into endothelial cells or SMCs . It has been suggested that stem cell differentiation into endothelial cells may diminish the thickness of neointima layers .…”

Section: Discussionmentioning

confidence: 99%

DJ‐1 Regulates Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle‐like Cells in Response to Sphingosylphosphorylcholine

et al. 2017

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Although multiple factors contribute to the differentiation of human mesenchymal stem cells (hMSCs) into various types of cells, the differentiation of hMSCs into smooth muscle cells (SMCs), one of central events in vascular remodeling, remains to be clarified. ROS participate in the differentiation of hMSCs into several cell types and were regulated by redox-sensitive molecules including a multifunctional protein DJ-1. Here, we investigated the correlation between altered proteins, especially those related to ROS, and SMC differentiation in sphingosylphosphorylcholine (SPC)-stimulated hMSCs. Treatment with SPC resulted in an increased expression of SMC markers, namely α-smooth muscle actin (SMA) and calponin, and an increased production of ROS in hMSCs. A proteomic analysis of SPC-stimulated hMSCs revealed a distinctive alteration of the ratio between the oxidized and reduced forms of DJ-1 in hMSCs in response to SPC. The increased abundance of oxidized DJ-1 in SPC-stimulated hMSCs was validated by immunoblot analysis. The SPC-induced increase in the expression of α-SMA was stronger in DJ-1-knockdown hMSCs than in control cells. Moreover, the expression of α-SMA, and the calponin and generation of ROS in response to SPC were weaker in normal hMSCs than in DJ-1-overexpressing hMSCs. Exogenous H O mimicked the responses induced by SPC treatment. These results indicate that the ROS-related DJ-1 pathway regulates the differentiation of hMSCs into SMCs in response to SPC.

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“…Specifics of the exact SC differentiation protocols is beyond the scope of this review and can be found elsewhere [21]. …”

Section: Advances In Stem Cell-derived Vascular Modelsmentioning

confidence: 99%

Stem cell-derived vasculature: A potent and multidimensional technology for basic research, disease modeling, and tissue engineering

Lowenthal

Gerecht

2016

Biochemical and Biophysical Research Communications

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Proper blood vessel networks are necessary for constructing and re-constructing tissues, promoting wound healing, and delivering metabolic necessities throughout the body. Conversely, an understanding of vascular dysfunction has provided insight into the pathogenesis and progression of diseases both common and rare. Recent advances in stem cell-based regenerative medicine – including advances in stem cell technologies and related progress in bioscaffold design and complex tissue engineering – have allowed rapid advances in the field of vascular biology, leading in turn to more advanced modeling of vascular pathophysiology and improved engineering of vascularized tissue constructs. In this review we examine recent advances in the field of stem cell-derived vasculature, providing an overview of stem cell technologies as a source for vascular cell types and then focusing on their use in three primary areas: studies of vascular development and angiogenesis, improved disease modeling, and the engineering of vascularized constructs for tissue-level modeling and cell-based therapies.

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Induced pluripotent stem cell-derived vascular smooth muscle cells: methods and application

Cited by 54 publications

References 82 publications

Vascular diseases await translation of blood vessels engineered from stem cells

Vascular diseases await translation of blood vessels engineered from stem cells

DJ‐1 Regulates Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle‐like Cells in Response to Sphingosylphosphorylcholine

Stem cell-derived vasculature: A potent and multidimensional technology for basic research, disease modeling, and tissue engineering

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