Induced pluripotent Stem Cells: Where we are currently?

Rančić, Nemanja; Raščanin, Sanja; Miljković, Milijana; Jovanovic, Mirjana

doi:10.5937/halo26-27861

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…Furthermore, genetic aberrations after reprogramming might bear tumorigenic potential and thus provoke carcinogenesis in the transplanted iPSC-derived tissues (174). Additionally, iPSC-derived tissues need to be manufactured according to SOPs and GMP guidelines which need lots of effort to implement those prerequisites into standard clinical care (175).…”

Section: Conclusion/outlookmentioning

confidence: 99%

Human Pluripotent Stem Cells Go Diabetic: A Glimpse on Monogenic Variants

et al. 2021

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Diabetes, as one of the major diseases in industrial countries, affects over 350 million people worldwide. Type 1 (T1D) and type 2 diabetes (T2D) are the most common forms with both types having invariable genetic influence. It is accepted that a subset of all diabetes patients, generally estimated to account for 1–2% of all diabetic cases, is attributed to mutations in single genes. As only a subset of these genes has been identified and fully characterized, there is a dramatic need to understand the pathophysiological impact of genetic determinants on β-cell function and pancreatic development but also on cell replacement therapies. Pluripotent stem cells differentiated along the pancreatic lineage provide a valuable research platform to study such genes. This review summarizes current perspectives in applying this platform to study monogenic diabetes variants.

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Section: Conclusion/outlookmentioning

confidence: 99%

Human Pluripotent Stem Cells Go Diabetic: A Glimpse on Monogenic Variants

et al. 2021

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“…The discovery of Induced Pluripotent Stem Cells (iPSCs) opened the possibilities for reprogramming adult somatic cells back to a pluripotent state in vitro by inducing a forced expression of specific transcription factors so-called Yamanaka factors (i.e.,Oct3/4,Sox2,Klf4, or using the similar set of four transcription factors so-called Thompsons's modification of Yamnaka factors (i.e.,Oct4,Sox2,Nanog,and Lin28) (TAKAHASHI, YAMANAKA, 2006;GURDON, 2006;TAKAHASHI et al, 2007;YU et al, 2007;YAMANAKA, 2008;LOWRY et al, 2008;LANZA, АTALA, 2014;RANČIĆ et al, 2020). These type of stem cells have common features with Embryonic Stem Cells (ESCs) in terms of surface antigen expression, telomerase activity, gene expression, proliferation, and morphology, also could be differentiated into the cells of all three germ layers (LAKO et al, 2010;RAŠČANIN et al, 2019;RANČIĆ et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…But it is very important to note that there is a risk of tumorogenesis. More research is needed to fully understand the reprogramming system and how iPSCs can be controlled to produce a sufficient number of cells with high quality and safety requirements for use in stem cells treatments (WAKUI et al, 2017;VOLAREVIC et al, 2018;RAŠČANIN et al, 2019;ZAKRZEWSKI, 2019;RANČIĆ et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Questionnaire for evaluating information, knowledge, and attitudes on donation, storage, and application of induced pluripotent stem cells

Rascanin

Jovanovic

Stevanović³

et al. 2021

Genetika

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The discovery of Induced Pluripotent Stem Cells (iPSCs) opened the possibilities for reprogramming adult somatic cells back to a pluripotent state in vitro by inducing a forced expression of specific transcription factors. Thus, iPSCs might have potential application in regenerative medicine, transplantation, avoidance of tissue rejection, disease modeling, and drug testing. Because of apparent ethical issues connected with donation and derivation of biomaterials, iPSCs are considered as a research alternative to ethically highly disputed Embryonic Stem Cells (ESCs). Objective: The aim of this paper was to describe the development of a questionnaire for evaluating information, knowledge, and attitudes on donation, storage, and application of iPSCs (i.e., the QIPSC). We performed a prospective qualitative study based on the development, validation and reliability testing of the QIPSC. The study included 122 respondents and the final version of the QIPSC with 34 items. The reliability analysis for part of information and knowledge of respondents according to iPSCs was then performed with the questions included in this two-component model and obtained a Cronbach's alpha value of 0.783 and 0.870, respectively. It has been shown that the range of correct answers to questions in part of knowledge of respondents according to iPSCs was from 17.2-63.1%. The results of our study show that the QIPSC was a unique, reliable, and valid questionnaire for assessing the level of information, knowledge, and attitudes on donation, storage, and application of iPSCs.

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Evaluation of knowledge on induced pluripotent stem cells among the general population and medical professionals

Rascanin,

Jovanovic,

Rancic

2023

Med pregl

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Introduction. Induced pluripotent stem cells are ethically much more acceptable than embryonic stem cells. The aim of this paper is to evaluate the knowledge on these cells among the general population and medical professionals, because this may influence further research. Material and Methods. We conducted a survey to assess knowledge on induced pluripotent stem cells among the general population and medical workers. The inclusion criteria were age over 18 years and the ability to read and write in Serbian. The survey was conducted via email and printed materials using a validated questionnaire for evaluation of awareness, knowledge, and attitudes towards donation, storage, and application of induced pluripotent stem cells. The collected data were entered into an Excel database, and complete statistical analysis was performed using the Statistical Package for the Social Sciences version 26.0. Results. The rate of correct answers among health workers ranged from 17.5% to 67.1%, and among the general population from 16.4% to 49.4%. The average number of correct answers per respondent in the population of health workers was 6, while in the general population it was 4, which is statistically significantly lower. Conclusion. The research results showed that healthcare workers have a higher level of knowledge on induced pluripotent stem cells than members of the general population, but the level of knowledge can be influenced by the level of education, availability of information, socioeconomic status, ideology, and conservative attitudes.

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Induced pluripotent Stem Cells: Where we are currently?

Cited by 4 publications

References 42 publications

Human Pluripotent Stem Cells Go Diabetic: A Glimpse on Monogenic Variants

Human Pluripotent Stem Cells Go Diabetic: A Glimpse on Monogenic Variants

Questionnaire for evaluating information, knowledge, and attitudes on donation, storage, and application of induced pluripotent stem cells

Evaluation of knowledge on induced pluripotent stem cells among the general population and medical professionals

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